32 results about "PARAQUAT POISONING" patented technology

The objective of the invention is to provide a Chinese herbal compound used for treating paraquat poisoning. According to the invention, Chinese traditional treatment theories of invigoration of blood, removal of stasis, elimination of necrotic tissue, engendering of flesh, nourishment to the lung, promotion of blood circulation, protection of the liver and the kidney and the like are used as support, and by nourishing and invigorating blood, human immunity is enhanced, self-repairing capacity and transporting and digesting functions of visceral organs are improved and formation of pulmonary fibrosis is inhibited. The Chinese herbal compound comprises gecko, Chinese caterpillar fungus, dysosma pleiantha, tendril-leaved fritillary bulb, Artemisia capillaris, rhizome anemones altaicae, cassia twig, white peony root, vegetable sponge of luffa, Eclipta alba, dark plum, ginseng, donkey-hide gelatin and furnace soil.

The invention provides a new use of sulfonatocarlix(5)arene in preparing a medicine for treating paraquat poisoning. The sulfonatocarlix(5)arene reduces probability of contacts between paraguat and NAD(P)H in vivo by inclusion coordination of the sulfonatocarlix(5)arene and the paraguat, and reduces oxidizing ability of the paraguat, thus inhibiting generation of a free radical (HO<.>) which is poisonous to cells in vivo. Additionally, the lower edge of the sulfonatocarlix(5)arene is rich in phenolic hydroxyl structures which can provide active hydrogen to inactivate the HO<.> free radical. Experiments on mice prove that the sulfonatocarlix(5)arene is nontoxic or low toxic and greatly reduces toxicity of the paraguat. After a mouse is poisoned by orally taking the paraguat (dose: 106.99mg / kg), good curative effect can be achieved by orally taking two-fold sulfonatocarlix(5)arene for once, the total lethality is decreased from 90% to 30%, and the lowest lethality is even decreased to 0%. After a mouse is poisoned, good effect can be achieved by orally taking the sulfonatocarlix(5)arene in two hours after poisoning.

The invention discloses a method for rapidly detecting paraquat in a biological matrix sample, which uses a real-time direct analysis ion source and a mass spectrometer for detection, does not treat the detected biological matrix sample, and is an in-situ detection method. Pretreatment of traditional means is omitted, and rapid and real-time detection of biological matrix samples poisoned by paraquat is achieved.

A preparation for treating oral mucositis caused by paraquat. The preparation particularly includes following components: 250ml of a 0.9% sodium chloride solution, 0.16g of 2% lidocaine, 0.005g of dexamethasone and 0.08g of gentamicin. The preparation can reduce oral mucositis damage of a paraquat poisoning patient, can alleviating pain, can resist infection and can inhibit immunity. The preparation is used in a manner of mouth wash. A patient uses the preparation in the mouth-washing manner per 2 hours in daytime so that stimulation during local drug delivery is avoided and pain is alleviated. By means of frequent mouth washing, oral cavity can be moistened and large residue blocks and necrotic tissues can be removed. The preparation can reduce secretion and dental plaque, promotes a self-cleaning function of the oral cavity. A mouth washing liquid is low in cost and has an important clinical application value.

The invention provides a toxicant qualitative and semi-quantitative detection kit and a toxicant qualitative and semi-quantitative method. A 5ml detection liquid centrifugal tube, a 5ml blank centrifugal tube, a reaction colourimetric card and a qualitative detection strip are arranged in the kit; the 5ml detection liquid centrifugal tube contains 3ml detection liquid; and the reaction colourimetric card is that detection liquid is added when in different paraquat concentrations. The toxicant qualitative method comprises the steps of: (1), adding a sample to be detected into the detection liquid, wherein the mass ratio of the sample to be detected to the detection liquid is 1:4 to 4:1; (2), if the colour of the mixed solution in the step (1) is changed, representing that the sample to be detected contains paraquat; and (3), when immersing the immersing end of the qualitative detection strip in the sample to be detected, instantly changing the colour in a reaction tank, and representingthat the sample to be detected contains paraquat. According to the toxicant qualitative and semi-quantitative detection kit and the toxicant qualitative and semi-quantitative method in the invention,by utilization of the principle that sodium hydrosulfite and paraquat perform colour changing reaction in an alkaline condition, the qualitative detection strip and the qualitative and semi-quantitative detection kit are prepared; a novel diagnosis method can be provided for clinically excluding paraquat poisoning; and the kit effectively serves in clinic, and has very high social value.

The invention discloses an application of glycosyl modified polyphenol compounds as drugs for relieving paraquat poisoning and treating pulmonary fibrosis. The glycosyl modified polyphenol compounds have the structure shown in the description. The glycosyl modified polyphenol compounds have a good oxidation resistance function and a lung protection function and have a notable protection function on lung injury caused by paraquat, and a mechanism is related with reduction of formation of oxygen radicals, relieving of oxidative stress and inflammatory reaction, increase of content of GSH in blood serum and SOD in lung tissue and reduction of content of MDA and HYP in lung tissue. The glycosyl modified polyphenol compounds can effectively inhibit bleomycin-induced pulmonary fibrosis of mice,and the mechanism is related with reduction of expression of TGF-beta 1, IL-6, alpha-SMA, P-smad2, type I collagen and type III collagen in lung tissue, increase of content of GSH and SOD in blood serum and reduction of the content of HYP in lung tissue.

The invention provides a new use of sulfonatocarlix(5)arene in preparing a medicine for treating paraquat poisoning. The sulfonatocarlix(5)arene reduces probability of contacts between paraguat and NAD(P)H in vivo by inclusion coordination of the sulfonatocarlix(5)arene and the paraguat, and reduces oxidizing ability of the paraguat, thus inhibiting generation of a free radical (HO<.>) which is poisonous to cells in vivo. Additionally, the lower edge of the sulfonatocarlix(5)arene is rich in phenolic hydroxyl structures which can provide active hydrogen to inactivate the HO<.> free radical. Experiments on mice prove that the sulfonatocarlix(5)arene is nontoxic or low toxic and greatly reduces toxicity of the paraguat. After a mouse is poisoned by orally taking the paraguat (dose: 106.99mg / kg), good curative effect can be achieved by orally taking two-fold sulfonatocarlix(5)arene for once, the total lethality is decreased from 90% to 30%, and the lowest lethality is even decreased to 0%. After a mouse is poisoned, good effect can be achieved by orally taking the sulfonatocarlix(5)arene in two hours after poisoning.

The patent of invention relates to a method for detecting the paraquat in urine, which are used for quickly detecting the most common pesticide poisoning, namely paraquat poisoning. The reagent comprises a reagent A and a reagent B, wherein the reagent A is sodium bicarbonate; and the reagent B is alkali sodium dithionite or sodium dithionite. The method for detecting the paraquat comprises the following steps of: taking 10ml of urine of a patient, putting the urine in a clean plastic cup, adding 2.0g of the reagent A into the cup, and after the reagent A is completely and uniformly dissolved, adding 1.0g of the reagent B into the cup, wherein if the urine contains paraquat ions, the urine immediately turns blue, and the darker the blue color is, the higher the concentration of the paraquat in the urine is. The reagent has the advantages of high accuracy, no false positive and false negative, high sensitivity, specificity and uniqueness.

The invention provides a human lung cancer paraquat-resistant cell strain. A human lung cancer cell strain A549 is used as an induction object; an in-vitro induction method of periodic exposure and progressive increase of paraquat concentration is adopted; and a paraquat-resistant cell strain A549 / PQ is built. The cell strain is collected in CGMCC (China General Microbiological Culture Collection Center) on June 16, 2015; and the collection number is CGMCC NO.10892. The cell strain has the typical paraquat-resistant characteristic; by using the biological characteristic, a study model can be provided for further studying a paraquat toxicity reversion path, extracting a paraquat poisoning evaluation marker, exploring a novel anti-oxidization path and the like; and high scientific research and production and application values are realized.

A human lung cancer cell strain A549 is adopted as an inducing object, an in-vitro inducing method for performing periodic exposure and gradually increasing the concentration of paraquat is adopted, and a paraquat-resistant cell strain A549 / PQ is established. The cell strain is preserved in China General Microbiological Culture Collection Center at June 16th 2015, and the preservation number is CGMCC NO.10892. The cell strain has the typical paraquat-resistant characteristic, and the biological characteristic provides a research model for further performing research on reversing of toxicity of paraquat, extracting an evaluation marker of paraquat poisoning and exploring a new anti-oxidant way, and has high scientific research value and production and application value.

The invention provides a human lung cancer paraquat-resistant cell strain. A human lung cancer cell strain A549 is used as an induction object; an in-vitro induction method of periodic exposure and progressive increase of paraquat concentration is adopted; and a paraquat-resistant cell strain A549 / PQ is built. The cell strain is collected in CGMCC (China General Microbiological Culture Collection Center) on June 16, 2015; and the collection number is CGMCC NO.10892. The cell strain has the typical paraquat-resistant characteristic; by using the biological characteristic, a study model can be provided for further studying a paraquat toxicity reversion path, extracting a paraquat poisoning evaluation marker, exploring a novel anti-oxidization path and the like; and high scientific research and production and application values are realized.

The invention relates to a kit for rapid determination of paraquat concentration in blood. The kit mainly comprises a reagent I for removing proteins, a reagent II for removing nucleic acid and preventing interference, a kit solvent, a paraquat standard and a paraquat calibrator. The invention also relates to an application method of the kit. The kit can rapidly determine the paraquat concentration in the blood of a patient, and provides rapid, simple, accurate, reliable and effective reference for clinical diagnosis of paraquat intoxication.