Reagent for qualitatively detecting paraquat and method for detecting paraquat in urine

A qualitative detection and detection method technology, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, so as to achieve the effects of high sensitivity, stability, high sensitivity and high accuracy.

Inactive Publication Date: 2010-11-10
赵永秀
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the deficiencies in the above technical aspects, the present invention provides a reagent for qualitat...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0013] For patients with paraquat poisoning through blood test, the concentration of paraquat in their blood is 5.6 micrograms / ml.

[0014] Take 10ml of urine from the patient above, put it in a pure plastic cup, first add 2.0g of reagent A, after fully mixing and dissolving, add 1.0g of reagent B, the urine immediately turns dark blue, indicating the concentration of paraquat in urine higher.

[0015] The above urine is accurately measured by chemical method, and the concentration of paraquat in the urine is: 50 micrograms / ml.

example 2

[0017] For patients with paraquat poisoning by blood test, the concentration of paraquat in their blood is 1.5 micrograms / ml.

[0018] Take 10ml of the patient's urine above, put it in a pure plastic cup, first add 2.0g of reagent A, after fully mixing and dissolving, add 1.0g of reagent B, the urine immediately turns light blue, indicating that there is paraquat in the urine The concentration is lower.

[0019] The above urine was accurately measured by chemical method, and the concentration of paraquat in the urine was 12 micrograms / ml.

example 3

[0021] For patients with paraquat poisoning by blood test, the concentration of paraquat in their blood is: 0.4 micrograms / ml.

[0022] Take 10ml of the patient's urine above, put it into a pure plastic cup, first add 2.0g of reagent A, after fully mixing and dissolving, add 1.0g of reagent B, the urine immediately turns slightly blue, indicating that there is paraquat in the urine The concentration is very low.

[0023] The above urine was accurately measured by chemical method, and the concentration of paraquat in the urine was 3 micrograms / ml.

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Abstract

The invention relates to a reagent for qualitatively detecting paraquat and a method for detecting the paraquat in urine, which are used for quickly detecting the most common pesticide poisoning, namely paraquat poisoning. The reagent comprises a reagent A and a reagent B, wherein the reagent A is sodium bicarbonate; and the reagent B is alkali sodium dithionite or sodium dithionite. The method for detecting the paraquat comprises the following steps of: taking 10ml of urine of a patient, putting the urine in a clean plastic cup, adding 2.0g of the reagent A into the cup, and after the reagent A is completely and uniformly dissolved, adding 1.0g of the reagent B into the cup, wherein if the urine contains paraquat ions, the urine immediately turns blue, and the darker the blue color is, the higher the concentration of the paraquat in the urine is. The reagent has the advantages of high accuracy, no false positive and false negative, high sensitivity, specificity and uniqueness.

Description

technical field [0001] This patent relates to a reagent for qualitative detection of paraquat and a detection method for paraquat in urine, which is used for the rapid diagnosis of one of the most common pesticide poisonings, paraquat poisoning. Background technique [0002] Paraquat is a kind of herbicide widely used in agriculture and forestry. Inadvertent contact in production and life can cause poisoning. This kind of poisoning is one of the poisonings with a higher incidence at present, and sometimes it is more difficult to diagnose, especially for patients with occult poisoning with unclear contact history. Although some patients have a clear history of exposure, it is often difficult to clearly judge the amount of exposure and the degree of poisoning. Although the "blood paraquat detection technology" currently used clinically can be qualitative and quantitative, the technology is very complicated, the detection time is long, and the cost is high. Therefore, there i...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 赵永秀
Owner 赵永秀
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