Method for rapidly detecting paraquat in biological matrix sample

A biological matrix and paraquat technology, applied in the field of detection and analysis, can solve the problems of unavoidable chromatography, a large amount of harmful waste liquid, and lengthy separation process, and achieve the effects of simple workflow, simplified clinical diagnosis, and reduced operating costs

Inactive Publication Date: 2020-03-20
ASPEC TECH LTD
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires complex pre-treatment and lengthy separation process, the analysis period is long and will produce a large amount of harmful waste liquid and pollute the environment
Mass spectrometry also cannot avoid some defects of chromatography itself due to the need for chromatographic separation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly detecting paraquat in biological matrix sample
  • Method for rapidly detecting paraquat in biological matrix sample
  • Method for rapidly detecting paraquat in biological matrix sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Instruments and reagents

[0029] API 4000 triple quadrupole mass spectrometer (Applied BioSystem, USA), real-time direct analysis ion source (DART, IonSense, USA). Paraquat standard samples and experimental plasma came from the Academy of Military Medical Sciences, and stomach content samples came from the Forensic Medicine Center of Beijing Municipal Public Security Bureau.

[0030] 1. The main ions of paraquat generated by DART source in positive ion mode are: [M-H-2Cl] + (m / z185), [M+H-2Cl] + (m / z 187), [M+H-CH 3 -2Cl] + (m / z 172) and [M+H-2CH 3 Cl] + (m / z 157). Among them, both m / z 185 and 157 can be used as parent ions (see Table 1 for mass spectrometry parameters)

[0031] Table 1 Paraquat mass spectrometry DART-QqQ parameters

[0032]

[0033] Instrument conditions

[0034] DART-MS ion source parameters: positive ion mode; grid voltage 100V; injection track moving speed 0.4mm / s; heater temperature 500°C;

[0035] Mass spectrometry parameters: positive...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for rapidly detecting paraquat in a biological matrix sample, which uses a real-time direct analysis ion source and a mass spectrometer for detection, does not treat the detected biological matrix sample, and is an in-situ detection method. Pretreatment of traditional means is omitted, and rapid and real-time detection of biological matrix samples poisoned by paraquat is achieved.

Description

technical field [0001] The invention relates to a detection and analysis technology, in particular to a method for rapidly detecting paraquat in a biological matrix sample. Background technique [0002] Paraquat (paraquat), also known as Gramoxone, has a strong destructive effect on green plant tissue, and is used as a rapid, broad-spectrum, contact herbicide and defoliant in agriculture. Paraquat is extremely toxic, with an oral lethal dose of 40mg / kg, and there is no specific antidote. If ingested into the body by mistake, paraquat will enter target organs such as the lungs and kidneys, causing lung fibrosis and kidney failure. Although paraquat has little side effects on the environment, more and more poisoning incidents have made it a serious threat to human life and property. At present, more than 20 countries have banned or strictly restricted the use of paraquat. Because of its high toxicity and widespread application, it is extremely important to establish an effi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/72G01N30/06
CPCG01N30/72G01N30/06
Inventor 段晓琨刘春胜
Owner ASPEC TECH LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap