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Method for preparing fermentable sugar and lignosulfonate from furfural residues

A technology of lignosulfonate and furfural slag, which is applied in the field of biochemistry, can solve problems such as the decrease of enzyme hydrolysis efficiency, and achieve the effects of reduced dosage, easy implementation, and good compatibility

Inactive Publication Date: 2013-08-28
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alkali treatment of furfural slag can remove part of lignin, but its enzymatic hydrolysis efficiency decreases instead

Method used

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  • Method for preparing fermentable sugar and lignosulfonate from furfural residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 10kg of furfural slag (industrial furfural slag) and adjust it to a solid content of 10% with water, soak for 24 hours, stir at regular intervals, filter, and rinse the filter residue with tap water 5 times until the pH of the rinse water is 7. Wash the obtained furfural slag for later use.

[0032] see figure 1 . Take 100g of furfural slag (furfural slag obtained by washing with water, the same below) and 80% ethanol solution, the solid content of the mixture is 100g / L, then add sodium sulfite (sodium sulfite concentration is 2g / L), and place in a 60°C 100rpm React in a constant temperature water bath shaker for 2.5 hours, then centrifuge the sample, the separated solid is used for countercurrent washing, and the separated liquid is retained.

[0033] The pretreatment steps were repeated to obtain five batches of solids, which were subjected to multistage countercurrent washing with 95% industrial ethanol. The first batch of solids was washed three times with 9...

Embodiment 2

[0037] Take 100g of furfural slag and use 80% ethanol solution to adjust the solid content to 100g / L, then add sodium sulfite (sodium sulfite concentration is 2g / L), place it in a constant temperature water bath shaker at 60°C and 100rpm for 2.5h, and then The sample was subjected to centrifugation, and the separated solids were sequentially countercurrent washed with detergent 4, detergent 3, detergent 2, detergent 1 and 95% ethanol solution of Example 1.

[0038] The samples after counter-current washing were washed with deionized water at 50°C, and then enzymatically hydrolyzed using an acetic acid-sodium acetate buffer system with a pH of 4.8. The enzymatic hydrolysis conditions were substrate concentration of 25g / L, and the amount of cellulase was 15FPU / ( g cellulose), the enzymatic hydrolysis temperature is 50℃, and the hydrolysis time is 68h.

[0039] The saccharification rate reached 84.1%. The lignosulfonate yield was 14.8%.

Embodiment 3

[0041] Take 100g of furfural slag and adjust it to a solid concentration of 100g / L with 75% ethanol solution, then add sodium sulfite (the concentration of sodium sulfite reaches 5g / L), put it in a constant temperature water bath shaker at 60°C and 100rpm for 3h, and then carry out the sample Centrifugal separation, the solid that separates obtains uses the washing agent 4 of embodiment 1, washing agent 3, washing agent 2, washing agent 1 and 95% ethanol solution countercurrent washing successively.

[0042] The samples after countercurrent washing were washed with deionized water at 50°C, and then subjected to enzymatic hydrolysis. , using acetic acid-sodium acetate buffer system with a pH of 4.8, the enzymatic hydrolysis conditions were substrate concentration 25g / L, cellulase dosage 15FPU / (g cellulose), enzymatic hydrolysis temperature 50°C, and hydrolysis time 65h.

[0043] The saccharification rate reached 83.5%. Lignosulfonate yield was 15.6%.

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Abstract

The invention provides a method for preparing fermentable sugar and lignosulfonate from furfural residues. The method comprises the following steps of 1, ethanol and sulfite-based pretreatment comprising that furfural residues reacts with sulfite in an ethanol solution and reaction products are subjected to solid-liquid separation, 2, countercurrent washing comprising that the solids obtained by the step 1 are subjected to multistage countercurrent washing by an ethanol washing liquid, 3, enzyme hydrolysis comprising that the solids subjected to the multistage countercurrent washing are subjected to cellulase hydrolysis, and 4, ethanol recovery comprising that the liquid obtained by the solid-liquid separation in the step 1 is mixed with the washing liquid recovered after the countercurrent washing in the step 2 and the mixed solution is subjected to pressure reduction condensation to form ethanol and lignosulfonate. The method utilizes combination of an organic solvent method and a sulfite method. Through the sulfite method, furfural residues are pretreated so that furfural residue sample hydrophily is improved and lignin removal and hydrolysis rate increasing are realized. Through the organic solvent method, the raw materials are pre-treated so that pretreatment liquid recovery and recycle are realized and a production cost is reduced.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a method for treating furfural slag by using enzymes. Background technique [0002] In recent years, in response to the "oil crisis" facing the world and the immeasurable impact on the environment caused by the use of petrochemical resources, people have gradually shifted their attention to the renewable resources on the earth-lignocellulosic raw materials. Using lignocellulosic materials as raw materials, cellulose can be converted into glucose through enzymatic hydrolysis, and can be converted into liquid fuels and chemical raw materials such as ethanol, butanol, lactic acid, acetone, glycerin, etc., which can replace part of petroleum resources, and can not only make human Get rid of excessive dependence on oil resources, and can significantly reduce pollution and greenhouse gas emissions. [0003] Furfural slag is biomass waste such as corncobs, corn stalks, rice husk...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C08H7/00
Inventor 蒋建新卜令习邢杨刘志平于海龙朱莉伟高玉霞
Owner BEIJING FORESTRY UNIVERSITY
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