Bacillus subtilis feed additive

A technology of Bacillus subtilis and feed additives, applied in the field of Bacillus subtilis feed additives and Bacillus subtilis feed additives, can solve the problems of no direct utilization of nutritional value of farmed animals, unsatisfactory compatibility and application, affecting the survival of bacteria, etc., to reduce the number of prawns. The effect of disease occurrence, enhancement of immunity, and improvement of economic benefits

Inactive Publication Date: 2018-11-23
JIANGSU QIANYAOTANG GUOYI RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to keep the pH value in the stomach of the animals relatively constant, so that the fed food can continue to achieve the effect of acidification after entering the small intestine, acidulants such as citric acid, lactic acid, hydrochloric acid, and fumaric acid are often used in the product at the same time, but these acidulants Not only does it directly cause excessive acidity to the part of the microorganisms contained in the additive, which affects the survival of the bacteria, but at the same time, these acidified substances have no nutritional value for direct use of the farmed animals. At the same time, they are harmful to the gastrointestinal The props are very irritating, and its compatible application is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Preparation of sea buckthorn freeze-dried powder, prickly pear freeze-dried powder, physalis freeze-dried powder, black currant freeze-dried powder, cactus freeze-dried powder:

[0057] Take freshly ripe sea buckthorn, prickly pear, physalis, black currant, and cactus, remove impurities such as fruit stems and rotten fruits, rinse with a 40°C aqueous solution containing 0.1% by weight of citric acid for 15 minutes under constant stirring, and put them in. Rinse 3 times in pure water, remove and control the moisture; put the sea buckthorn, prickly pear, physalis, black currant, and cactus on a plate and transfer them to the freezer, and quickly cool down to below -45 °C; respectively freeze the sea buckthorn, prickly pear , physalis, blackcurrant and cactus are moved into the sublimation dryer, the sublimation temperature is controlled at -22--32°C, and the vacuum degree is 120-150Pa, so that the material is dried to moisture 100 mesh to obtain sea buckthorn freeze-dried ...

Embodiment 2

[0059] Using the corresponding optimized medium, the liquid culture fermentation equipment is used to separately cultivate Candida utilis, Candida tropicalis, brewing yeast, brewer's yeast and feed yeast, and then use a high-speed centrifuge to separate the bacteria in the fermentation broth, With the vacuum freeze-drying mode, the bacterium mud is dried to obtain the dry powder of Candida utilis and the dry powder of Candida utilis that the live bacteria content is 20 billion / gram respectively, the dry powder of Candida tropicalis that the live bacteria content is 20 billion / gram, the live bacteria content is 200. 100 million / gram of brewing yeast dry powder, 20 billion / gram of live bacteria content of brewer's yeast dry powder and 20 billion / gram of live bacteria content of feed yeast dry powder.

Embodiment 3

[0061] Preparation of fermented eggshell powder:

[0062] Wash egg shells or one or several mixed egg shells of duck egg shells, goose egg shells and quail egg shells, dry them, put them in a far-infrared dryer, dry them with hot air at 180°C for 2 hours, and take them out. Let cool, pulverize into 45 mesh powder; weigh 80 kg of eggshell powder, add an appropriate amount of pure water to make the moisture content of the material at 50% (W), then add 20 kg of dry feed yeast powder obtained in Example 2, and mix well After loading, put it into the fermentation room, and ferment it for 60-72 hours at a temperature of 31-33°C. After the fermentation is completed, adjust the fermentation material to a temperature of 82°C-92°C and a temperature of 80-90%. , carry out the carbonyl ammonia reaction for 22-28 days, then dry the material in an electric oven under the condition of not more than 100 ℃ to a moisture content of less than 5% (W), use a universal pulverizer to pulverize, pass...

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PUM

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Abstract

The invention discloses a bacillus subtilis feed additive. The bacillus subtilis feed additive is characterized by being prepared through the steps: uniformly mixing bacillus subtilis, fructus hippophae freeze-dried powder, roxburgh rose freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder and cactus freeze-dried powder, then adding residual fermented eggshell powder and uniformly mixing, and carrying out compound packaging; in every 1000g of the bacillus subtilis feed additive, the quantity of the bacillus subtilis is 1000 billion to 12000 billion, the content of the fructus hippophae freeze-dried powder is 5g to 50g, the content of the roxburgh rose freeze-dried powder is 5g to 50g, the content of the wintercherry freeze-dried powder is 3g to 30g, the content of the blackcurrant freeze-dried powder is 3g to 30g, the content of the cactus freeze-dried powder is 50g to 150g, and the balance is the fermented eggshell powder. The bacillus subtilis feedadditive disclosed by the invention is a single type bacillus subtilis feed additive, is clear in variable bacteria quantity, and is beneficial for clear application and quantitative selection of users; the fructus hippophae freeze-dried powder, the roxburgh rose freeze-dried powder, the wintercherry freeze-dried powder, the blackcurrant freeze-dried powder, the cactus freeze-dried powder and thefermented eggshell powder are utilized as matrixes of the bacillus subtilis feed additive, so that the nutritive value of the bacillus subtilis feed additive is increased, meanwhile, the metabolism ofanimals is promoted, the disease resistance of a body is increased, growth of the animals is promoted, and the economic benefit of breeding industry is finally increased.

Description

technical field [0001] The invention belongs to the field of feed additives, and relates to a Bacillus feed additive used for breeding animals. Specifically, it relates to a Bacillus subtilis feed additive. Background technique [0002] In the gastrointestinal tract, Bacillus mainly maintains the intestinal ecological balance through biological oxygen capture. After short-term colonization of the intestinal tract, it can consume a large amount of oxygen to maintain the intestinal anaerobic environment and enhance the intestinal colonization of anaerobic bacteria. In addition, Bacillus can convert starch into monosaccharides in the gut, and then other bacteria in the gut convert these monosaccharides into lactic acid, lowering the pH of the gut, thereby inhibiting pathogenic bacteria. [0003] Bacillus is significantly different from probiotic products made by other microorganisms. Bacillus can form spores under unfavorable conditions, protect itself, and have a high resurre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K10/30A23K10/26A23K10/12
CPCA23K10/12A23K10/18A23K10/26A23K10/30
Inventor 张奎昌张志年
Owner JIANGSU QIANYAOTANG GUOYI RES INST CO LTD
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