Method for preparing organic seaweed liquid fertilizer by fermentation and enzymolysis of microorganisms

A seaweed liquid, seaweed technology, applied in the direction of organic fertilizers, etc., can solve problems such as the destruction of active nutrients, and achieve the effects of promoting growth and development, improving photosynthetic efficiency, and improving soil structure

Inactive Publication Date: 2018-08-10
泰安达沃斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, most of the enterprises that produce seaweed fertilizer by biodegrading seaweed...

Method used

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  • Method for preparing organic seaweed liquid fertilizer by fermentation and enzymolysis of microorganisms
  • Method for preparing organic seaweed liquid fertilizer by fermentation and enzymolysis of microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing organic seaweed liquid fertilizer by microbial fermentation and enzymolysis, the specific preparation steps are as follows:

[0028] (1) Fresh kelp raw materials are washed with water to remove sand grains and other impurities;

[0029] (2) Add pure fresh water accounting for 1.2 times the quality of kelp to 50 g of washed kelp, crush and grind;

[0030] (3) Put the crushed kelp slurry into the fermentation container, add 2.5×10 9 CFU of Bacillus subtilis and 2.5 x 10 9 CFU of Bacillus amyloliquefaciens (the ratio of the bacteria amount is 1:1), after stirring evenly, heat to 37°C, and keep the fermentation for 48h;

[0031] (4) After fermentation, the kelp slurry turns from green to brown, and the pH value is less than 6. Add 0.6g of acid protease and cellulase (2:1) to it, stir evenly, heat to 55°C, and keep it warm for 3 hours. ;

[0032] (5) Filtrate the solution after enzymolysis to obtain a supernatant, and concentrate to obtain an organ...

Embodiment 2

[0034] A method for preparing organic seaweed liquid fertilizer by microbial fermentation and enzymolysis, the specific preparation steps are as follows:

[0035] (1) Fresh kelp and wakame (3:1) are washed with water to remove sand and other impurities;

[0036] (2) Add pure fresh water accounting for 2 times the quality of seaweed to 50 g of mixed seaweed after washing, crush and grind;

[0037] (3) Add the seaweed slurry into the fermentation vessel, add 2.5×10 9 CFU of Bacillus subtilis and 2.5 x 10 9 CFU of Bacillus licheniformis (the ratio of the amount of bacteria is 1:1), stirred evenly, heated to 37°C, and kept for 48 hours of fermentation;

[0038] (4) Add 0.6g of acid protease and pectinase (1:1) to the fermented seaweed slurry, stir evenly, heat to 55°C, and keep warm for 3 hours;

[0039] (5) Filtrate the solution after enzymolysis to obtain a supernatant, and concentrate to obtain an organic seaweed liquid fertilizer.

Embodiment 3

[0041] A method for preparing organic seaweed liquid fertilizer by microbial fermentation and enzymolysis, the specific preparation steps are as follows:

[0042] (1) Wash fresh kelp and Enteromorpha (3:1) raw materials with water to remove sand and other impurities;

[0043] (2) Add pure fresh water accounting for 3 times the quality of seaweed to 50 g of mixed seaweed after washing, crush and grind;

[0044] (3) Add the seaweed slurry to the fermentation vessel, add 4×10 9CFU of Bacillus amyloliquefaciens and 4 x 10 9 CFU of Bacillus licheniformis (the ratio of the amount of bacteria is 1:1), stirred evenly, heated to 37°C, and kept for 48 hours of fermentation;

[0045] (4) Add 0.5 g of seaweed quality pectinase and cellulase (3:1) to the fermented seaweed slurry, stir evenly, heat to 55°C, and keep warm for 3 hours;

[0046] (5) Filtrate the solution after enzymolysis to obtain a supernatant, and concentrate to obtain an organic seaweed liquid fertilizer.

[0047] Acco...

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Abstract

The invention discloses a method for preparing organic seaweed liquid fertilizer by fermentation and enzymolysis of microorganisms and belongs to the field of fertilizer production. Seaweeds are degraded through a compound enzymolysis technology and a microorganism fermentation technology; a complicated enzyme system produced through microorganism fermentation and a compound enzyme including proteinase, cellulase, pectinase, polysaccharide degradation enzyme and the like are used for degrading the seaweeds step by step; seaweed cell walls are removed so that contents including alginic acid, polysaccharide, plant hormones and the like are sufficiently released; the decomposition of active substances including the alginic acid, plant growth promotion factors and the like is avoided under moderate technological condition; effective active components in the seaweeds are kept to the greatest extent. According to the method disclosed by the invention, energy saving, water saving, low carbonand environment protection are realized; the seaweed biological organic liquid fertilizer contains rich mineral substances and effective components including macro-elements, microelements and the likeneeded by the growth of plants; a soil structure can be improved to keep moisture and soil and the growth of root microorganisms of the plants is promoted; the yield of applied crops is remarkably improved and the fertilizer has a great market potential.

Description

technical field [0001] The invention relates to a method for preparing organic seaweed liquid fertilizer through microbial fermentation and enzymolysis, belonging to the field of fertilizer production. Background technique [0002] my country is a large agricultural country, but the input of chemical fertilizers used in traditional agriculture for a long time has caused waste of resources and environmental pollution, making soil compaction worse and worse, and the yield and quality of crops have decreased instead. The resulting agricultural products Residual problems have threatened the health of Chinese people. my country is still a country that lacks water resources. There are 420 million mu of arable land in dry and semi-arid areas. The use of ordinary chemical fertilizers is difficult to solve these urgent problems. It is necessary to fundamentally solve the problem of unsustainability in my country's agricultural production. The key measures are to develop new plant nutr...

Claims

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Application Information

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IPC IPC(8): C05F11/00
CPCC05F11/00
Inventor 蔡冬梅刘向伟何雁林于文新
Owner 泰安达沃斯生物科技有限公司
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