AFB1 degrading bacterium and degrading enzyme

A technology for degrading enzymes and degrading bacteria, applied in the direction of enzymes, fungi, enzymes, etc., can solve problems such as adverse effects

Active Publication Date: 2013-02-13
HENAN PUAI FEED GRP CO LTD +1
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  • Application Information

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Problems solved by technology

On December 24, 2011, the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China announced the results of the sampling inspection of liquid milk products nationwide. A batch of products produced by Mengniu Dairy (Meishan) Co., Ltd. were found to exceed 140% of the standard for aflatoxin M1. The reason was that the dairy cows ate mildewed feed, which caused a sensation all over the country and caused serious adverse effects.

Method used

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[0024] AFB 1 Screening of degrading bacteria: AFB 1 Dissolve it into a solution with a concentration of 100 μg / L, and then evenly spread it on the PDA solid medium; according to the liquid-solid ratio mL / g, the decaying organic matter is subjected to 10 5 Diluted twice, and then evenly coated on the surface containing AFB 1 Cultured on the PDA solid medium at 29°C for 6 days. Observe the changes of the colonies every day. Among them, the earliest and better growing colonies appear, which are considered to be the target single colony (with strong aflatoxin decomposition ability) AOYIN2012Y8, in China The registration number of the Microbial Culture Collection is CGMCC No. 5817, and its morphological characteristics are: the strain grows rapidly on PDA medium, and the existing hyphae grow in a large amount at 24 h of solid fermentation culture, and then a large number of spores are produced. At 72 h, the medium was covered with spores and turned yellow-brown. Pick the hypha and ob...

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Abstract

The invention belongs to the technical field of biology, and discloses an AFB1 degrading bacterium and an AFB1 degrading enzyme. The degrading bacterium is aspergillusoryzane AOYIN2012Y8 with a collection number of CGMCC No.5817. The aspergillusoryzane AOYIN2012Y8 CGMCC No.5817 is inoculated into a PDA solid culture medium, and constant-temperature culturing is carried out under a temperature of 28-30 DEG C; collection is carried out when a lot of spores grows. The spores are scraped off by using an inoculation loop. The spore concentration is regulated to 1.0*10<8>CFU/mL by using normal saline containing 0.5-0.7% Tween 80. 5-7mL of the spore is inoculated to every 30g of a solid fermentation culture medium. Constant-temperature culturing is carried out for 5-7 days under a temperature of 28-30 DEG C, and collection is carried out. The solid fermentation culture medium is well mixed with normal saline, and the mixture is subjected to room-temperature soaking. The mixture is filtered and centrifuged; a supernatant is fetched and is subjected to salting-out, dialysis, and gel chromatographic separation; and concentrating and lyophilizing are carried out, such that the AFB1 degrading enzyme is obtained. The degrading enzyme provided by the invention has relatively high decomposing capability against AFB1, wherein a maximal degradation rate reaches 80.12%.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to an AFB 1 Degrading bacteria and degrading enzymes. Background technique [0002] Since the outbreak of mycotoxin disease in turkeys in the United Kingdom in 1960, a large number of investigations and studies on mycotoxins have been carried out around the world. Currently, more than 300 species of fungi are known to produce mycotoxins. The main mycotoxins include: aflatoxin, zearalenone, ochratoxin, trichothecenes, and citrinin. These toxins are widely distributed and have been isolated from a large number of feed ingredients and mixed feeds. Among various mycotoxins, aflatoxin is considered the most serious and toxic, because it can cause liver poisoning, and has strong immunosuppressive, carcinogenic, mutagenic and teratogenic properties. [0003] According to the data of the Food and Agriculture Organization of the United Nations (FAO) in 2002, about 25% of the world's grains ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/00C12R1/69
Inventor 尹清强左瑞雨王平刘俊熙王潇常娟郑秋红
Owner HENAN PUAI FEED GRP CO LTD
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