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Bacillus cereus strain, pyrethroid pesticide degrading enzyme prepared using same and preparation method of pyrethroid pesticide degrading enzyme

A technology of Bacillus cereus and pyrethroids, applied in the field of microorganisms, can solve the problems of not being able to meet the needs of pest control in pollution-free tea gardens, and little research

Inactive Publication Date: 2009-12-02
洪永聪 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research and development of biological pesticides are far from meeting the needs of pest control in pollution-free tea gardens, and there are few studies on the direct application of endophytic bacteria to control tea plant diseases at home and abroad.

Method used

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  • Bacillus cereus strain, pyrethroid pesticide degrading enzyme prepared using same and preparation method of pyrethroid pesticide degrading enzyme
  • Bacillus cereus strain, pyrethroid pesticide degrading enzyme prepared using same and preparation method of pyrethroid pesticide degrading enzyme
  • Bacillus cereus strain, pyrethroid pesticide degrading enzyme prepared using same and preparation method of pyrethroid pesticide degrading enzyme

Examples

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Effect test

Embodiment 1

[0030] Isolation and screening of TR2 strain

[0031] After surface treatment of healthy and diseased leaves of different tea tree varieties by plant surface disinfection, endophytic bacteria were isolated with different culture media. Bacterial isolation media were NA, KB and TSA. Bacterial proliferation medium is NA or NB. The medium used for fungal culture and antagonism assay was modified PDA. The basal medium with cypermethrin as the sole carbon source was used for the determination of pesticide degradation. Randomly pick a single colony according to the shape and color of the colony, purify it according to the conventional method, and save it for testing and identification. The antagonism of all isolated strains to tea leaf spot fungus and their growth ability on cypermethrin plates were determined.

[0032] Identification of endophytic bacteria

[0033] Refer to the method of Dong Xiuzhu et al. (Dong Xiuzhu, Cai Miaoying: Common Bacteria Identification Manual [M], ...

Embodiment 2

[0070] Preparation of pyrethroid pesticide degrading enzyme from Bacillus cereus TR2 strain (CCTCC NO: M209001)

[0071] The culture medium involved in this embodiment: the preservation of the degrading bacteria adopts the NA medium supplemented with cypermethrin (50 mg / L). Degradation efficiency of degrading bacteria was determined by adding cypermethrin (50mg / L) to the basal medium.

[0072] Involved in this embodiment for testing pesticides: 10% cypermethrin emulsifiable concentrate (Nanjing Red Sun Group Co., Ltd.), 2.5% cyfluthrin emulsifiable concentrate (Suzhou Fumeishi Plant Protection Agent Co., Ltd.), 20% fenpropathrin emulsifiable concentrate (Zhejiang Weir Da Chemical Co., Ltd.), 40% dimethoate EC (Chongqing Minfeng Agrochemical Co., Ltd.), 80% dichlorvos EC (Jiangsu Meilan Group Co., Ltd.), 70% methamidophos crude oil and 40% chlorpyrifos EC (Shandong Huayang Technology Co., Ltd.), cypermethrin standard sample (Beijing Kanglin Technology Co., Ltd.).

[0073] Pre...

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Abstract

The invention discloses a bacillus cereus strain, which is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO:M209001. The invention also discloses a pyrethroid pesticide degrading enzyme prepared by using the bacillus cereus strain as an initial strain and a method for preparing the same. The strain is a bacillus cereus which is separated from the body of a tea plant and has high pyrethroid pesticide degradation efficiency. The intracellular crude enzyme of the strain has high efficiency in the degradation of pyrethroid pesticides such as cypermethrin, cyfluthrin and fenpropathrin, but low efficiency in the degradation of organophosphorus pesticides such as cygon, panaplate, methamidophos, chlorpyrifos and the like.

Description

technical field [0001] The invention relates to a Bacillus cereus strain, especially a Bacillus cereus strain endogenous to tea trees that can efficiently degrade pyrethroid pesticides, and also relates to a pyrethroid pesticide degrading enzyme and its Preparation. It belongs to the technical field of microorganisms. Background technique [0002] With the continuous improvement of people's living standards and the enhancement of health awareness, green food is more and more popular. Tea is a natural health drink and is known as a healthy drink in the 21st century. Nearly half of the people in the world drink tea. However, due to the direct or indirect damage of pests and diseases, it has a certain negative impact on improving the economic benefits of tea. Tea is an economic crop whose main harvest is young shoots. Traditional chemical control methods have brought serious problems such as excessive pesticide residues and ecosystem deterioration to tea products. In my cou...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/16A62D3/02C12R1/085A62D101/04A62D101/20
Inventor 洪永聪王军强纪国才兰孝帮
Owner 洪永聪
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