Cetirizine molecularly imprinted polymer monolithic column and preparation method thereof

A cetirizine and molecular imprinting technology, which is applied in the field of preparation of molecularly imprinted monolithic columns, can solve problems such as non-volatile, and achieve the effects of easy operation, simple preparation process and reduced operation time.

Inactive Publication Date: 2014-12-17
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with general ionic liquids, choline-based deep eutectic solvents have more prominent features, such as non-volatile, low toxicity and degradable, low price, easy to obtain and easy to prepare, etc., and have attracted the attention of many scholars in recent years. , but there is no report on the preparation of MIP using deep eutectic solvents

Method used

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  • Cetirizine molecularly imprinted polymer monolithic column and preparation method thereof
  • Cetirizine molecularly imprinted polymer monolithic column and preparation method thereof
  • Cetirizine molecularly imprinted polymer monolithic column and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation and separation of cetirizine and its six-membered heterocyclic triazine structural analogs containing nitrogen atoms on a molecularly imprinted monolithic column for cetirizine

[0028] A molecularly imprinted monolithic column with cetirizine as template was synthesized by in situ polymerization, and its retention performance was evaluated by connecting it to high performance liquid chromatography under appropriate chromatographic conditions. Synthetic reaction conditions and processing method are as follows:

[0029] Preparation of cetirizine molecularly imprinted monolithic column based on deep eutectic solvent by in situ polymerization:

[0030] a. According to the mass number of cetirizine imprinted monolithic column synthesized in a stainless steel column, accurately weigh the template molecule as cetirizine 1.56%, the functional monomer as 4-VP (4-vinylpyridine) 3.43%, The crosslinking agent is 23.4% ethylene glycol dimethacrylate, and the porogen is...

Embodiment 2

[0034] Embodiment 2 (control)

[0035] Separation of Cetirizine and Its Structural Analogues on a Blank Monolithic Column

[0036] In order to investigate the retention of cetirizine and its structural analogues on non-imprinted columns, a blank column without template cetirizine was synthesized as a control. The specific operation steps are as follows:

[0037] A non-imprinted blank column was synthesized with the same method and experimental conditions as in Example 1, except that the template molecule cetirizine was not added. After polymerization is complete, flush with acetonitrile to remove residual porogen and unreacted reagents from the monolith.

[0038] The chromatographic evaluation is the same as the investigation of the imprinted column in Example 1, that is, under the same mobile phase conditions, by measuring the retention time t of cetirizine and its six-membered heterocyclic triazine analogs containing nitrogen atoms R and retention time t of acetone 0 , t...

Embodiment 3

[0042] Preparation of cetirizine molecularly imprinted monolithic column by in situ polymerization:

[0043] a. According to the mass number of cetirizine imprinted monolithic column synthesized in a stainless steel column, weigh 1.56% of cetirizine, 3.43% of 4-VP (4-vinylpyridine) as the functional monomer, and 3.43% of the crosslinking agent as Ethylene glycol dimethacrylate 24.3%, porogen is a deep eutectic solvent composed of ethylene glycol dissolved in choline chloride 44.1%, DMF (dimethylformamide) 4.28%, ionic liquid (1-butyl base-3-methylimidazolium tetrafluoroborate) 20.4%, then add 0.99% cobalt acetate and 0.94% azobisisobutyronitrile as the initiator to mix, put it into the ultrasonic machine, and ultrasonicate for 20 minutes to make it uniform. Clarify, then transfer to a clean stainless steel column (1004.6 mm I.D.), ultrasonically degas for 15 minutes, seal both ends of the stainless steel column, and react in a constant temperature water bath at a temperature o...

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Abstract

The invention relates to a cetirizine molecularly imprinted polymer monolithic column and a preparation method thereof. The cetirizine molecularly imprinted polymer monolithic column is composed of raw materials of, by mass, 1-3% of cetirizine, 3-4% of 4-vinyl pyridine, 20-25% of ethylene gylcol dimethacrylate, 42-45% of mixed solution (eutectic solvent) of ethylene glycol and choline chloride, 4-5% of dimethylformamide, 13-22% of 1-butyl-3-methylimidazolium tetrafluoro borate and 0.5-1% of azobisisobutyronitrile. The method includes the steps of adding ionic liquid and the eutectic solvent of ethylene glycol and choline chloride to a polymerization system, using cobalt ions as a metal ion hub to enhance the effect of imprinting recognition, and preparing molecularly imprinted polymer (MIP) of a successive rod shape in a stainless steel column. The cetirizine molecularly imprinted polymer (MIP) monolithic column obtained through the preparation method has the advantages of good permeability and obvious imprinting effects and can have the model cetirizine imprinting factors up to 31.54, and meanwhile, the method is simple in preparation process and avoids the use of volatile liquid, thereby reducing harmful gas emission to the environment.

Description

technical field [0001] The present invention relates to the preparation of a molecularly imprinted monolithic column, in particular to a cetirizine molecularly imprinted monolithic column and a preparation method thereof, specifically to prepare a cetirizine molecularly imprinted monolithic column by using a deep eutectic solvent in-situ polymerization method The column and its preparation method can be applied to the field of molecular recognition. Background technique [0002] Molecular imprinting technology refers to the technology of using a specific molecule as a template to prepare materials with special recognition function and high selectivity for the molecule. It is usually described as an "artificial lock" technology for creating a "molecular key" for identification. It dissolves template molecules, monomers, and cross-linking agents in a certain solution to form a pre-polymerization solution, and forms a three-dimensional space with functional groups that complet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/285B01J20/30B01D15/22
Inventor 刘照胜孙璇李秀媛黄艳萍
Owner TIANJIN MEDICAL UNIV
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