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Tetragenous diagnosis kit for food born parasite disease of human body

A diagnostic kit and technology for parasitic diseases, applied in the field of kits for rapid diagnosis of human foodborne parasitic diseases, can solve problems such as early diagnosis of unfavorable patients, long time for detection methods, waste of resources, etc.

Inactive Publication Date: 2004-01-07
ZHEJIANG ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] The present invention mainly solves the technical problems that the existing detection method is limited to a single detection for a long time, which not only causes waste of resources, but also is unfavorable for early diagnosis of patients.

Method used

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Embodiment Construction

[0007] The technical solutions of the present invention will be further specifically described below through examples.

[0008] The reaction device consists of three parts: one is a small flat box, which is divided into the bottom of the box and the box cover. There is a round hole with a diameter of 0.3-0.5cm on the box cover; , Cellulose acetate mixed film.

[0009] Preparation of solid-phase spotting membrane: cut the mixed cellulose membrane of nitric acid and acetic acid into a disc with a diameter of 1 cm, add an arrow-shaped quality control point in the center of the disc (use 1.5 μl of 1:10 human serum for spotting), and place the arrow up and down. Add 1 μl of lung fluke adult antigen, liver fluke adult antigen, toxoplasma tachyzoite antigen, and porcine cysticercoid crude antigen in a clockwise direction from left to right, and store in a refrigerator at 4°C after drying.

[0010] Preparation of chloroauric acid-labeled staphylococcal protein A in the chromogenic sy...

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Abstract

The invention refers to a multi-connection rapid diagnosing reagent box, including reaction board, antigen solid-phase film, golden mark coloration system, and buffering system; the antigens: 1% lung-fluke imaglo soluble antigen, liver fluke imago antigen, arched worm rapid-breeding sub-ectoblast antigen, and pig bursa cercaria coarse antigen; the solid-phase; nitric acnd acetic acid mixed fibrinfilm, aperture 0.65 micron m; the coloration system, auric chloride acid mark staphylococcus A albumen; the buffering system includes phosphate buffering solution (PSB), pH 7.2 and concentration 0.02mol / L and bull serum albumen (BSA), the content 1-5%, as well as Tris buffering solution (TBS), the content 0.5-1%, and pH 8.2.

Description

technical field [0001] The invention relates to a diagnostic kit, in particular to a kit for rapidly diagnosing human food-borne parasitic diseases. Background technique [0002] With the rapid development of society and the acceleration of urbanization, people's eating habits have undergone great changes. At the same time, due to factors such as cooking, the incidence of human food-borne parasitic diseases has increased, and parasitic disease inspection is required. The number of people has also increased, and it is not uncommon for one serum to be tested for multiple parasitic diseases. The diagnostic kits in the prior art can only do a single test, not multiple tests at the same time, and take a long time, which not only causes waste of resources but also is not conducive to early diagnosis of patients. Contents of the invention [0003] The invention mainly solves the technical problems that the existing detection method is limited to a single detection for a long tim...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/543G01N33/566G01N33/569
Inventor 林怡
Owner ZHEJIANG ACAD OF MEDICAL SCI
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