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Rapid detection chromatography technology for pathogenic bacteria in water environment

A pathogenic bacteria and water environment technology, applied in the direction of measuring devices, analytical materials, biological testing, etc., can solve the problem of pathogenic bacteria that have not been seen yet, and achieve the effect of rapid detection and simple detection operation

Inactive Publication Date: 2010-12-29
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report on the detection of common pathogenic bacteria in water environment by domestic immunochromatographic strips

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] The preparation of the colloidal gold-labeled immune paper chromatography strip of the present invention firstly collects common pathogenic bacteria in water, namely Salmonella, Shigella, Staphylococcus aureus, Vibrio parahaemolyticus, etc., for preparing antigens, using the above-mentioned antigens Immune white rabbits for antibody preparation, and then purify the antibody to prepare immunocolloidal gold, bind the immunocolloidal gold to the probe conjugate pad, and place the colloidal gold probe conjugate pad of common pathogenic bacteria in water on nitrocellulose One end of the membrane and the other end are provided with a water-absorbing plate pad, and a control line and a bacterial antibody detection line are fixed in sequence between the two pads. .

[0021] The method for detecting pathogenic bacteria in water environment using colloidal gold-labeled immunopaper chromatography strips of the present invention will be further described with reference to the examp...

Embodiment 1

[0022] Embodiment 1: the detection of artificially dyed Vibrio parahaemolyticus sea and river water

[0023] Take 20 parts of 100mL Haihe water, put them in a clean conical flask, and add 10 parts respectively after autoclaving. 8 , 10 7 , 10 6 , 10 5 cfu / mL standard strains of Vibrio hemolyticus (20516, 20572, 20502, 20507, 20562) at different concentrations. The detection method is as follows: place the immunochromatographic test strip in the sample to be tested (the height should not exceed the colloidal gold conjugate pad in the test strip), and when the sample liquid swims to the absorption plate pad, take out the parahemolysis Vibrio immunochromatography test paper, placed for 5 minutes to observe the results. The results showed that the detection limit of strains 20516, 20572 and 20502 could reach 10 in the contaminated Haihe water sample. 6 cfu / mL; while less sensitive for 20507 and 20562, 10 7 cfu / mL.

Embodiment 2

[0024] Embodiment 2: Detection of artificially dyed Salmonella sea and river water

[0025] Take 16 parts of 100mL Haihe water, put them in a clean conical flask, and add 10 parts respectively after autoclaving. 8 , 10 7 , 10 6 , 10 5 Salmonella standard strains (50708, 50096, 50210, 50315) at different concentrations in cfu / mL. The detection method is as follows: place the immunochromatographic test strip in the sample to be tested (the height should not exceed the colloidal gold conjugate pad in the test strip), and when the sample liquid swims to the absorption plate pad, take out the Salmonella immunoassay. Chromatography test paper, placed for 5 minutes to observe the results. The results showed that the detection limit of strains 50708 and 50096 could reach 10 6 cfu / mL; while less sensitive for 50210, 10 7 cfu / mL; for Salmonella 50315, when the bacterial suspension concentration is 3.4×10 5 At cfu / mL, the detection line is faintly visible.

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PUM

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Abstract

The invention discloses an immunologic paper chromatographic strip for specifically and rapidly detecting common pathogenic bacteria in a water environment and a specific and rapid detection method thereof. The immunologic paper chromatographic strip of the invention which is used for detecting the common pathogenic bacteria in the water environment is new technology and application for rapidly detecting the common pathogenic bacteria in water. At present, a large number of reports on detection of soluble antigen by colloidal gold immunochromatographic test paper are available at home and abroad; and the bacteria serving as particulate antigen are extracted or processed by other methods and then detected abroad. In the technology, a special chromatographic film is selected and used and other conditions are optimized, so that the immunochromatographic strip can be directly used for detecting the bacteria. The technology can be used for rapidly detecting the common pathogenic bacteria in the water environment.

Description

technical field [0001] The invention relates to the rapid detection of common pathogenic bacteria in water, more particularly, to an immunopaper chromatography strip and a detection method for rapid detection of common pathogenic bacteria in water by using colloidal gold and immunochromatography technology. Background technique [0002] Water is a substance that human beings rely on for survival. It is not only used for drinking and living water, but also a discharge place for human and animal body excrement, production and domestic sewage, etc. Therefore, there are various microorganisms in water. The greatest threat to human health in water is pathogenic bacteria: historically, there have been many worldwide cholera pandemics; Cholera is prevalent; in addition, outbreaks of other bacterial diseases (such as typhoid fever, bacillary dysentery, diarrhea, etc.) are also common due to natural disasters, wars, sanctions, deterioration of sanitary conditions, and poor drinking w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/551G01N33/52
Inventor 金敏李君文王新为谌志强邱志刚陈照立王景峰
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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