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Adjuvant for improving immersion immunization effect of fishery vaccines and application of adjuvant

A fish vaccine and adjuvant technology, which is applied in the direction of medical formula, medical preparations containing active ingredients, antibody medical ingredients, etc., can solve the problems of high labor intensity and high cost, and achieve improved immunity, simple application methods, and procedures simple effect

Inactive Publication Date: 2015-06-03
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The immunization effect of fish vaccine injection delivery is good, but the cost is high and the labor intensity is high. It is suitable for application in industrial farming, but not suitable for application in ponds and cages in my country. However, immersion immunization is easy to operate, especially suitable for small-sized fish Species are immune during pond division, transportation, etc.

Method used

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  • Adjuvant for improving immersion immunization effect of fishery vaccines and application of adjuvant
  • Adjuvant for improving immersion immunization effect of fishery vaccines and application of adjuvant
  • Adjuvant for improving immersion immunization effect of fishery vaccines and application of adjuvant

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The synthetic method of embodiment 1 castor oil polyethylene glycol monoester glucoside

[0023] The synthesis process of castor oil polyethylene glycol monoester glucoside is as follows: the castor oil is acidified first, then esterified to produce castor oil polyethylene glycol 400 monoester, and finally glycosidized to obtain castor oil polyethylene glycol monoester Glucoside; the specific operation process is as follows:

[0024] 1) Acidification: Put castor oil and concentrated sulfuric acid into the reaction kettle according to the volume ratio of 10: (0.5-1.5) by weight, stir and heat up. When the temperature rises to 105°C, take a sample to check the pH value of the lower layer solution. Then use castor oil to adjust the pH value to 2-3, keep warm for 0.5 hours, stop heating and stirring, let it stand for 0.5-1 hour, put the acid solution in the lower layer into a storage container or reuse it, add an equal volume of Wash with tap water, wash repeatedly until t...

Embodiment 2～5

[0027] Embodiment 2~5: a kind of adjuvant of fish soak vaccine

[0028] The formulations of the fish soaking vaccine adjuvants described in Examples 2-5 are shown in Table 1 (the values ​​in the table are all parts by mass).

[0029] The formula of the fish soaked vaccine adjuvant described in table 1 embodiment 2~5

[0030]

[0031] The fish soaking vaccine adjuvants described in Examples 2-5 can be obtained by mixing the components in Table 1 according to the corresponding parts by mass.

Embodiment 6

[0032] Example 6 The application method of fish soaked vaccine adjuvant

[0033] How to use fish soaking vaccine adjuvant:

[0034] 1) Add the fish soaking vaccine adjuvant prepared in any one of the above Examples 2 to 5 into tap water and stir, mix well, and the obtained solution is soaking immune solution 1;

[0035] 2) Mix the soaking adjuvant solution 1 obtained in the above 1) and the vaccine solution evenly at a volume ratio of 1: (100~200), and the obtained mixed solution is the soaking immune solution 2, and the final concentrations of each component are: castor oil Polyethylene glycol monoester glucoside 1~20 μg / mL, azone 0~10 μg / mL, anisodamine 0~30 μg / mL;

[0036] 3) Put the fish body to be immunized into the immersion immune solution 2, inflate and soak for 15-30min, and the immunization is completed.

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Abstract

The invention discloses an adjuvant for improving immersion immunization effect of fishery vaccines and an application of the adjuvant. The adjuvant is castor oil polyethylene glycol monoester glucoside or a composition of castor oil polyethylene glycol monoester glucoside, azone and anisodamine. The application of the adjuvant comprises the following steps: dissolving an appropriate amount of adjuvant into water and then uniformly mixing with a vaccine solution in certain volume ratio, putting to-be-immune fishes into the mixed solution, filling gas and soaking for 15-30 minutes. According to the adjuvant disclosed by the invention, the castor oil polyethylene glycol monoester glucoside has good effects of biodegradability, non-toxicity, low interface tension, good water solubility, effects of dispersing, emulsifying and the like, so that oils or in-soluble antigens which are not dissolved in water are uniformly dispersed, and the immune effects of the vaccines are effectively improved. The application method of the adjuvant is simple, safe to apply, environment-friendly and free of adverse interferences on growth of fishes in culture production.

Description

technical field [0001] The invention relates to the administration route and application effect of the fishery vaccine, in particular to an adjuvant for improving the soaking immune effect of the fishery vaccine and an application method thereof. Background technique [0002] my country is a large freshwater aquaculture country with a high degree of intensification and serious aquatic diseases. Vaccine is an effective means of disease prevention, it can stimulate the animal body to produce antibodies, it can make fish specifically resist the attack of pathogens, while improving the specific immune level of fish, it can also enhance the body's ability to resist adverse stress, as an environmentally friendly The disease prevention and control measures of the sustainable development strategy have become one of the standard production specifications of the international modern aquaculture industry. During the immersion immunization process of fish vaccines, bacterial antigens n...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61P37/04
Inventor 任燕吴淑勤孙保兴石存斌
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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