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Specific skin regent for diagnozing tuberculomyces infection and active tuberculosis

A tuberculosis and active technology, applied in the field of medical immunology diagnosis, can solve problems such as research reports of tuberculosis skin allergens that have not yet been used in humans.

Inactive Publication Date: 2005-10-19
THE 309TH HOSPITAL OF CHINESE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on its use as a human tuberculosis skin allergen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Preparation of specific skin reagents for diagnosis of tuberculosis infection and active tuberculosis:

[0019] (1) Mycobacterium tuberculosis recombinant ESAT6 protein cloned, expressed and purified by genetic engineering technology is used as tuberculosis allergen;

[0020] (2) Preparation of diluent: 16g sodium chloride, 0.4g potassium chloride, 2.88g disodium hydrogen phosphate, 0.48g potassium dihydrogen phosphate, 6g phenol in 1500ml distilled water, stir vigorously on a magnetic stirrer, add 10ul Polysorbate 80, add hydrochloric acid to adjust the pH value to 7.2, add double distilled water to make up to 2000ml.

[0021] (3) diluting the recombinant ESAT6 protein of Mycobacterium tuberculosis with diluent so that its concentration is 1 Sug / ml;

[0022] (4) After mixing, sterilize and filter, test, put into a container, 1ml / bottle, and store at 4°C in the dark for later use (if stored properly, it can maintain activity for one year).

[0023] 2. The applicati...

Embodiment 2

[0027] 1. Preparation of specific skin reagents for diagnosis of tuberculosis infection and active tuberculosis:

[0028] (1) Using natural Mycobacterium tuberculosis ESAT6 protein as tuberculosis allergen;

[0029] (2) Preparation of diluent: 16g sodium chloride, 0.4g potassium chloride, 2.88g disodium hydrogen phosphate, 0.48g potassium dihydrogen phosphate, 6g phenol in 1500ml distilled water, stir vigorously on a magnetic stirrer, add 10ul Polysorbate 80, add hydrochloric acid to adjust the pH value to 6.8, add double distilled water to make up to 2000ml.

[0030] (3) Dilute the ESAT6 protein with a diluent so that its concentration is 6ug / ml;

[0031] (4) After mixing, sterilize and filter, test, put into a container, 1ml / bottle, and store at 4°C in the dark for later use (if stored properly, it can maintain activity for one year).

[0032] 2. Application of the skin diagnostic reagent of the present invention:

[0033] (1) Take 0.1ml of the above-mentioned skin diagnost...

Embodiment 3

[0036] 1. Preparation of specific skin reagents for diagnosis of tuberculosis infection and active tuberculosis:

[0037] (1) Mycobacterium tuberculosis recombinant ESAT6 protein cloned, expressed and purified by genetic engineering technology is used as tuberculosis allergen;

[0038] (2) Preparation of diluent: 16g sodium chloride, 0.4g potassium chloride, 2.88g disodium hydrogen phosphate, 0.48g potassium dihydrogen phosphate, 6g phenol in 1500ml distilled water, stir vigorously on a magnetic stirrer, add 10ul Polysorbate 80, add hydrochloric acid to adjust the pH value to 7.0, add double distilled water to make up to 2000ml.

[0039] (3) diluting the recombinant ESAT6 protein of Mycobacterium tuberculosis with a diluent so that its concentration is 10ug / ml;

[0040] (4) After mixing, sterilize and filter, test, put into a container, 1ml / bottle, and store at 4°C in the dark for later use (if stored properly, it can maintain activity for one year).

[0041] 2. The applicat...

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PUM

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Abstract

The present invention relates to specific skin reagent for diagnosing tuberculous infection and active tuberculosis, and belongs to the field of medical immunological diagnosis technology. The specific skin reagent consists of diluent liquid and dissolved tuberculous allergen, and the tuberculous allergen is tuberculous mycobacterium ESAT6 protein. The present invention also proposes applying tuberculous mycobacterium ESAT6 protein for human skin test as tuberculous allergen to detect tuberculous infecting person and tuberculosis patient and identify the specific reaction of inoculated Calmette-Guerin bacillus vaccine. The present invention can induce the immune response of tuberculous infecting person. The reagent can induce delayed allergic reaction of tuberculous infecting person and can identify BCG inoculation from the allergic reaction of non-tuberculous mycobacterium and tuberculous mycobacterium infection.

Description

technical field [0001] The invention belongs to the technical field of medical immunology diagnosis, and particularly relates to the application of ESAT6 protein of mycobacterium tuberculosis as a skin allergen in the detection of tuberculosis infection. Background of the invention [0002] Tuberculosis is still one of the major infectious diseases that endanger human health in the world. Since 1985, the prevalence of AIDS, tuberculosis-infected immigrants and some people living in poverty have caused the incidence of tuberculosis to rise in the United States and other developed countries in Europe and the United States, especially tuberculosis. The problem of bacterial drug resistance and the incidence of non-tuberculous mycobacterial disease are increasing year by year, which makes the treatment of tuberculosis even worse. At present, there are about 20 million tuberculosis patients in the world, 8 to 10 million new tuberculosis patients are added every year, and the annua...

Claims

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Application Information

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IPC IPC(8): A61K39/04A61P43/00
Inventor 吴雪琼张灵霞张翠英张俊仙史迎昌朱琳
Owner THE 309TH HOSPITAL OF CHINESE PEOPLES LIBERATION ARMY
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