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Kit for distinguishing and diagnosing hepatocirrhosis and early liver cancer and application of serum clusterin in preparing same

A technique for differential diagnosis and early liver cancer, which is applied in the field of cancer diagnosis, can solve problems such as false positives, false negatives, and limited sensitivity of AFP, and achieve the effects of strong specificity, simple operation, and easy wide-scale application

Inactive Publication Date: 2010-03-17
SUN YAT SEN UNIV
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Problems solved by technology

[0004] Serum alpha-fetoprotein (alpha-fetoprotein, AFP) has always been the best indicator for the diagnosis of liver cancer. However, the sensitivity of AFP is limited. Well-differentiated or poorly differentiated HCC often does not produce AFP, and AFP also has a certain degree in patients with liver cirrhosis. The positive rate of AFP has false negative and false positive clinically

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  • Kit for distinguishing and diagnosing hepatocirrhosis and early liver cancer and application of serum clusterin in preparing same
  • Kit for distinguishing and diagnosing hepatocirrhosis and early liver cancer and application of serum clusterin in preparing same
  • Kit for distinguishing and diagnosing hepatocirrhosis and early liver cancer and application of serum clusterin in preparing same

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[0015] Material:

[0016] Serum samples include: 25 cases of normal physical examination population without infection of HBV, HCV, HIV, and no tumor serum samples tested by clinical laboratory; 42 cases of serum samples of HBV carriers, including 20 cases of large three positive patients and 22 cases of small three positive patients ; Serum samples from 31 patients with liver cirrhosis. Serum samples from 76 patients with primary hepatocellular carcinoma (HCC). Including 22 cases of small liver cancer (diameter10cm). And, among all the tested HCC samples, AFP≤25μg / L accounted for 31 cases, and AFP>25μg / L accounted for 45 cases. Serum samples were taken in the morning on an empty stomach, centrifuged and stored at -80°C

[0017] The kit contains: 1. Human clusterin recombinant monoclonal antibody coated on a solid-phase 96-well microtiter plate;

[0018] 2. Positive control is human clusterin standard protein, negative control is standard negative serum; 3. Sample diluent: ...

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Abstract

The invention discloses a kit for diagnosing primary hepatoma, preparation method and application thereof. The kit can quickly and accurately carry out the clusterin detection so as to carry out quickauxiliary diagnosis on the primary hepatoma of each period; and the kit especially has an important clinical application value for distinguishing hepatocirrhosis and early liver cancer. The kit comprises an enzyme-linked immunological diagnosis system established on the basis of a double-antibody sandwich principle. The kit has high sensitivity, strong specificity, simple operation, stable reagent, good repeatability, easy popularization and application and extensive market prospect.

Description

[technical field] [0001] The invention relates to a cancer diagnosis technology, in particular to a differential diagnosis kit for liver cirrhosis and early liver cancer and the application of serum clusterin in the preparation of the kit. [Background technique] [0002] Primary liver cancer refers to the canceration that occurs in liver cells and intrahepatic bile duct epithelial cells, and is one of the most common malignant tumors in humans. Among them, primary hepatocellular carcinoma (Hepatocellular carcinoma, HCC) accounts for about 80%-90%. my country is an area with a high incidence of liver cancer. Since the 1990s, the incidence of liver cancer in my country has risen to the second place in malignant tumors, and the annual death rate accounts for the second to third place in the death rate of malignant tumors. 45%. In recent years, the incidence of HCC tends to increase, and the age of onset tends to be younger, and its development is rapid and its metastasis is ea...

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Application Information

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IPC IPC(8): G01N33/577G01N33/543
Inventor 谢丹孔祥复王毅朱萧贺龙君武鸿美廖奕佶
Owner SUN YAT SEN UNIV
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