44 results about "CYP2C9*3" patented technology

The invention relates to a gene chip used for detecting gene mutation relating to a high blood pressure personalized medicine. The gene chip for detecting the gene mutation relating to high blood pressure personalized medicine comprises a solid phase support, a gene probe (an oligonueleotide probe) fixed in the solid phase support sequentially and a PCR primer used for amplifying the mutated gene fragment in the sample; the gene probe (the oligonueleotide probe) and the PCR primer are designed aiming at one or two points of the gene mutation in ACE (I/D) and CYP3A5*3 and / or two or more points as follows: CYP2C9*3, CYP2C9*13, AGTR1(A1166C), CYP2D6*10, ADRB1(C1165G), TSC(C1784T), ADRB3(T727C), SCNN1G_rs5729, SCNN1G_rs5723, ENOSA_rs1799983 and GNB3(C825T). The invention provides the gene chip and applications thereof for conveniently, quickly and systematically detecting the gene mutation relating to high blood pressure personalized medicine so as to determine drug reactions.

The invention discloses a primer and probe composition for detecting polymorphism of human CYP2C9 and VKORC1 genes, a kit and application. The primer and probe composition comprises three pairs of specific primers for amplifying CYP2C9*2, CYP2C9*3 and VKORC1 sites, and three specific fluorescent probes. The primers and the probes, disclosed by the invention, have high sensitivity, strong specificity and strong anti-interference capability; a manner combining an asymmetric PCR (Polymerase Chain Reaction) amplification and fluorescent probe melting curve analysis technologies is used for detecting the gene polymorphism; different gene types can be effectively distinguished according to the quantity and Tm value of a melting peak; a result is convenient, clear and objective to judge and read.Single-tube sampling can be used for simultaneously detecting 6 mutation types of 3 gene sites; the primer and probe composition is simple and convenient to operate and the detection efficiency is improved; a large batch of samples can be detected and clinical operation is facilitated.

The invention relates to a hypertension gene polymorphism fluorescent PCR solubility curve detecting kit and application thereof, and a hypertension medication gene multiple-PCR solubility curve detecting method. The hypertension gene polymorphism fluorescent PCR solubility curve detecting kit mainly comprises a primer combination, a probe combination, and PCR reaction agents such thermally activated polymerases, PCR buffer solution and dNTPs (deoxy-ribonucleoside triphosphates) and involves polymorphism detection of 7 hypertension-related genes including CYP2D6*10, CYP2C9*3, ADRB1(1165G)C), AGTR1(1166A)C), CYP3A5*3, NPPA(T2338C) and ACE(I/D); the three reaction agents are applied to perform PCR amplification and solubility analysis to determine the related polymorphism of the 7 genes to guide clinical customized application of 5 majors types of hypertension drugs. The hypertension gene polymorphism fluorescent PCR solubility curve detecting kit can easily complete detection through 3reaction tubes, and meanwhile, save a nucleic acid extraction process, simplify operation steps and achieve a simple interpretation method.

The invention discloses a kit and a method for detecting the gene polymorphism related to warfarin personalized medication by a pyro sequencing method. The kit is used for typing genes related to the warfarin medication, and the single nucleotide polymorphism of VKORC1-1639 G>A (rs9923231) and CYP2C9*31075A>C(rs1057910) is involved; and the kit comprises primers shown as SEQ ID NO.3-8. By the kit, VKORC1-1639G>A and CYP2C9*3 1075A>C can be detected accurately and quickly in high flux, so that the safe, reasonable and effective personalized administration of the warfarin medication is realized.

The invention relates to a kit for rapidly detecting warfarin individualized medication related gene SNP sites, and its detection method, and belongs to a genetic detection technology in the clinic detection technique of the biomedical field. The genetic typing of two polymorphic sites comprising CYP2C9*3(1061A/C) and VKORC1(-1639G/A) is rapidly and accurately carried out through human peripheral blood DNA drawing, specific PCR amplification by a Taq man probe and fluorescence signal analysis. The invention provides primers, probes and kits which are used for detecting the polymorphic sites, a use of the primers and the probes in the preparation of the kits, and a use of the determination of the warfarin application amount of a patient according to the detection results of the polymorphic sites. The determination use can effectively reduce the generation of the warfarin application amount related adverse events and prevent thrombotic diseases. The detection method can be used for the auxiliary diagnosis and treatment of various patients needing warfarin clinically.

The invention discloses a primer group and a test kit for detecting the polymorphism of related genes of hypertensive drugs. The primer group is characterized by containing the following primers: CYP2D6*10-F, CYP2D6*10-R, ADRB1-F, ADRB1-R, AGTR1-F, AGTR1-R, ACE-F, ACE-R, CYP2C9*3-F and CYP2C9*3-R. The genes of CYP2C9*3, ADRB1 (1165G is greater than C), AGTR1 (1166A is greater than C), CYP2D6*10 and ACBL (I/D) disclosed by the invention are proved to be closely related to the efficacy of common drugs for treating hypertension; in addition, the rational and safety use of drugs for hypertension patients can be guided according to detection results of polymorphism site of the genes.

The invention relates to a primer pair for detecting CYP2C9 genetic typing through a pyrosequencing method and a kit, and belongs to the technical field of in vitro nucleic acid detection. The primer pair comprises a CYP2C9*2 forward amplification primer, a CYP2C9*2 reverse amplification primer, a CYP2C9*2 sequencing primer, a CYP2C9*3 forward amplification primer, a CYP2C9*3 reverse amplification primer and a CYP2C9*3 sequencing primer. Biotin labeling is conducted at the 5' end of the CYP2C9*2 forward amplification primer and the 5' end of the CYP2C9*3 reverse amplification primer. The kit comprises the amplification primers, a PCR reaction solution 1, a PCR reaction solution 2, the sequencing primers, uracil DNA glycosylase and Taq polymerase. The kit has the advantages of being accurate in detection result, high in specificity, short in detection period, easy to operate and capable of effectively meeting the clinical examination requirement.

The invention discloses a primer, a probe and a kit for detecting gene polymorphism of CYP2C9 and VKORC1. The primer comprises a gene detection specificity primer sequence of three loci YP2C9*2, CYP2C9*3 and VKORC1 and a probe sequence (SEQ ID NO.1-12). An ARMS primer is used to differentiate field and mutant genes, so that the primer has the advantages of high sensitivity and high specificity and is suitable for various sample types and high in detection result accuracy. In addition, the ARMS primer is quick and simple to synthesize, low in synthesis cost and better in amplification effect, and production cost can be lowered effectively. The kit is high in detection speed and quite convenient, and the whole detection process only takes 90 minutes. The whole kit does not contain toxic and harmful substances, thereby being harmless to operating personnel and environment and suitable for wide application in clinic detection and the like.

The invention discloses a kit for rapidly detecting CYP2C9*3 gene polymorphism. The kit comprises a PCR (Polymerase Chain Reaction) reaction mixing solution which comprises the following raw materials by final concentration: 0.05-0.12 U/microliter of DNA polymerase, 0.2 mM of dNTPs, 1 X of 5 X PCR buffering solution, 1.5-3.5 mM of MgCl2, 0.0005-0.015% (w/v) of lauryl sodium sulfate, 0.001-0.03% (w/v) of polyethylene glycol octylphenol ether, 0.5-1 micron of a forward primer, 0.5-1 micron of a reverse primer, 0.5-1 micron of a mutant molecular beacon, and 0.5-1 micron of a wild type molecular beacon; and the PCR reaction mixing solution is used for detecting cell samples. The primer, a Taqman-MGB probe and the kit for rapidly detecting the CYP2C9*3 gene polymorphism and the detection method of the CYP2C9*3 gene polymorphism are simple to operate, free from pollution, and rapid to detect.

The invention provides a detection kit for guiding warfarin dosage with high precision. The kit comprises amplification primers containing VKORC1 (1639G/A), CYP4F2(V433M), GGCX rs11676382, CYP2C9*2, CYP2C9*3, CYP2C9*5 and CYP2CP*6, a PCR reagent and a RFLP reagent. The invention also provides a detection method of the kit. According to the invention, the optimal dosage of warfarin of a patient can be accurately predicated, so as to assist doctors in using warfarin more reasonably and safely, thus reaching the steady INR value as far as possible, reducing the risks of bleeding and thrombus in the early stage of administration, and improving the treatment effect.

The invention discloses a quick genotyping method for guiding Warfarin utilization starting dose and a kit and belongs to the gene detection technology in clinical detection technology in the biomedicine field. According to the quick genotyping method, the deoxyribonucleic acid (DNA) of a host cell gene group is extracted, and the gene types of CYP2C9*3(1061 A/C) and VKORCI (-1639 G/A) of a subject are measured. The CYP2C9 *3(1061 A/C) and VKORCI (-1639 G/A) genes have a nucleic acid sequence site shown as SEQ ID 1, wherein the site is one of the two most main sites associated with Warfarin metabolism. The invention provides the quick and accurate genotyping method and the associated kit. The method is quick and simple in operation, and low in cost, can be applied to prevention, auxiliary diagnosis and treatment on thrombotic associated diseases, and is conveniently popularized and used in basic medical institutions.

The invention discloses a primer set capable of synchronously detecting related gene polymorphism of an anticoagulant drug. The primer set comprises a PCR amplification primer and a SNaPshot PCR primer; the detected sites include VKORC1-1639>A, CYP4F2*3, CYP2C9*3, CYP2C19*2, CYP2C19*3 and CYP2D6*10. Compared with a general method for individually amplifying a plurality of sites, the primer used for detecting has the advantages that the amplification processes are decreased, and the amplification cost is reduced; ,moreover, the high sensitivity, the high accuracy and the high precision can be ensured, the method reliability is ensured, and a doctor can accurately select drugs for patients and reasonably adjust the dosage of the drug.

The invention belongs to the technical field of biology and relates to a primer-probe combination and a kit for detecting associated genotyping of warfarin medication. The invention provides primers and probes for detecting associated genotyping of warfarin medication. The sequences of the primers are shown as SEQ ID NO.1-SEQ ID NO.6, and the sequences of the probes are shown as SEQ ID NO.7-SEQ ID NO.12. The primer-probe combination and the kit for detecting associated genotyping of warfarin medication have the advantages that development and application of the gene detection kit are guided through warfarin medication, typing of the three genes of CYP2C9*3, CYP4F2 and VKORC1 is facilitated and easily achieved, and noninvasive diagnosis is guaranteed to enable clinicians to master patient heredity conditions rapidly and accurately, decrease medical accident incidence caused by inappropriate medicine dose greatly, reduce medication side effect and lay the foundation for improvement on clinical treatment effect; since only about 2 hours is needed for detection of every sample, high detection speed is achieved.

The invention relates to the technical field of in vitro diagnosis, in particular to a kit for detecting polymorphism of human CYP2D6, CYP2C9, ADRB1, AGTR1, ACE genes by a multiple fluorescent PCR method. The kit is used to detect CYP2D6*10, CYP2C9*3, ADRB1 (1165G > C), AGTR1 (1166A > C), ACE (I/D) polymorphism sites. The primer and the probe have high sensitivity and high specificity, and can accurately detect genomic DNA as low as 0.1 ng/<mu>L. The kit is easy to operate, and can cooperate with an automated instrument for detection.

The invention discloses a kit for rapid detection of polymorphism of a Warfarin metabolic enzyme gene by virtue of a pyrosequencing method. The kit consists of three pairs of specific amplification primers and three sequencing primers; the three pairs of specific amplification primers include two genes, namely a CYP2CP gene and a VOKRC1 gene, wherein the CYP2CP gene contains two pairs of primers; the three sequencing primers specifically relate to three single nucleotide polymorphisms, namely CYP2C9*2, CYP2C9*3 and VKORC1 (-1693); and the nucleotide sequences are as shown in SEQ ID NO.1-9. The detection method disclosed by the invention is not only high in sensitivity, but also direct in result, and simpler, rapider and more accurate in interpretation; the kit is capable of achieving accurate, rapid and high-throughput detection of the Warfarin metabolic enzyme gene; and the kit is relatively high in popularization and application values in personalized medicine.

The invention discloses an ARMS primer for detecting CYP2C9 gene polymorphism. Aiming at sequence changes in every SNP of CYPC2C9*2(rs1799853, C430T, Arg144Cys) and CYP2C9*3(rs1057910, A1075C, Ile359Leu), the specificity ARMS primer and shared primers are designed. Mismatched base group (base group is marked with underline) is introduced to the second or third place of the 3' terminal end of the specificity ARMS primer so as to enlarge efficiency gap generating when specificity ARMS primer 3' terminal base are complementary and are not complementary to the SNP locus base on the DNA template, thus sharply improving specificity and accuracy of the detection.

The invention discloses a gene detection kit for administration of angiotensin II receptor inhibitors. Specific amplification primers and sequencing primers are designed for the polymorphic angiotensin II receptor inhibitors of two genes CYP2C9 * 3 and AGTR1 (A1166C); and the kit comprises the following components of an amplification reaction solution, a CYP2C9 * 3 sequencing primer, an AGTR1 (A1166C) sequencing primer and a positive control. According to the kit, gene polymorphism related to administration of the angiotensin II receptor inhibitors is detected by using a combination of asymmetric multiplex PCR amplification and an optimized pyrosequencing technology, the kit can simultaneously detect gene polymorphism of the CYP2C9 * 3 and the AGTR1 (A1166C), the sequencing primer is a compound of carboxyl modifier agarose gel and an amino-labeled DNA sequence, the kit can be used as a sequencing primer and a capture probe, the gene polymorphism can be rapidly and accurately detected, a result is conveniently and clearly interpreted, the administration of the angiotensin II receptor inhibitors can be guided from the gene level, and a gene-oriented suggestion can be provided for clinical personalized administration.

The invention relates to a CYP2C9 genotype detecting primer-probe set. The primer-probe set comprises primers and a probe of CYP2C9*2, primers and a probe of CYP2C9*3 and primers and a probe of internally-controlled GAPDH. The invention further relates to a CYP2C9 genotype detecting kit. The kit comprises a PCR reaction solution 1, a PCR reaction solution 2, a PCR reaction solution 3, a PCR reaction solution 4, a positive control substance and a blank control substance. The kit provided by the invention has high sensitivity up to one ten thousandth and the minimum detection limit of only 1-2 copies, and is especially applicable to detection of low-content mutant samples (such as serum or plasma); compared with a sequencing method, the invention has the advantages as follows: results obtained in the invention can be observed in real time, gel electrophoresis detection on a product is not required, the risks in contaminating the PCR product are effectively reduced through completely-closed operation, and the detection speed is high; therefore, the primer-probe set and the kit are suitable for high-throughput sample detection.

The invention provides a warfarin medication gene detection kit. The warfarin medication gene detection kit comprises a primer pair used for detecting polymorphism of to-be-detected loci of CYP2C9 * 2, CYP2C9 * 3, CYP4F2 * 3 and VKORC1 genes, probes, an inhibitor, a PCR reaction liquid, a positive quality control product and a negative quality control product; and the to-be-detected loci are at least one of an rs1799853 locus of CYP2C9 * 2 (430C>T) gene, an rs1057910 locus of CYP2C9 * 3 (1075A>C) gene, an rs2108622 locus of the CYP4F2 * 3 (1297G>A) gene and an rs9923231 locusof the VKORC1 (-1639G>A) gene. The invention also provides a use method of the warfarin medication gene detection kit. The problems of high sample treatment requirement, long detection period, complex operation, high cost, low specificity and the like during gene polymorphism are solved.

The invention discloses a primer group for detecting polymorphism of a human cytochrome P450 related gene. The primer group comprises specific primers and probes aiming at gene sites of CYP2C19*2, CYP2C19*3, CYP3A4*18, CYP2C9*3, CYP2D6*10 and CYP3A5*3. The invention further relates to a kit for detecting polymorphism sites of the human cytochrome P450 related gene. The kit comprises PCR (Polymerase Chain Reaction) reaction liquid freeze-dried powder (PCR buffer, dNTPs, MgCl2, UNG enzyme, Taq enzyme and trehalose) with the primers and the probes. The invention further relates to a method for detecting polymorphism sites of the human cytochrome P450 related gene. By adopting the method, a DNA (Deoxyribonucleic Acid) extraction procedure is avoided, micro whole-blood amplification is directlyimplemented, a small amount of blood is used, and patients can be relieved from pain; the time and the labor can be saved, and in addition, economic expense can be greatly reduced.

The invention relates to primer pairs and a kit for detecting warfarin medication related gene polymorphism, and belongs to the technical field of in vitro nucleic acid detection, wherein the primer pair comprises amplification primers respectively for alleles such as VKORC1-1639, CYP2C9*2 and CYP2C9*3, and GAPDH internal reference gene, and the kit comprises PCR reaction solutions containing theamplification primers. According to the present invention, the kit has high sensitivity and is suitable for the detection of low-content mutation samples, wherein the sensitivity can be up to 1/10000,and the minimum detection limit is only 1-2 copies; compared to the sequencing method, the method of the present invention has the following advantages that the detection result can be real-timely observed, the product does not require gel electrophoresis detection, and the completely closed tube operation is performed so as to effectively reduce the risk of PCR product contamination; and the kitfurther has the fast detection speed, and is suitable for high-throughput sample detection.