CYP2C9 genotype detecting primer-probe set and kit

A primer-probe, genotyping technology, applied in recombinant DNA technology, microbial assay/inspection, biochemical equipment and methods, etc. problems, to achieve the effect of fast sequencing, short reaction time, and simple sample processing
CN106350587AInactive Publication Date: 2017-01-25CHANGSHA 3G BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHANGSHA 3G BIOTECH
Publication Date
2017-01-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a CYP2C9 genotype detecting primer-probe set. The primer-probe set comprises primers and a probe of CYP2C9*2, primers and a probe of CYP2C9*3 and primers and a probe of internally-controlled GAPDH. The invention further relates to a CYP2C9 genotype detecting kit. The kit comprises a PCR reaction solution 1, a PCR reaction solution 2, a PCR reaction solution 3, a PCR reaction solution 4, a positive control substance and a blank control substance. The kit provided by the invention has high sensitivity up to one ten thousandth and the minimum detection limit of only 1-2 copies, and is especially applicable to detection of low-content mutant samples (such as serum or plasma); compared with a sequencing method, the invention has the advantages as follows: results obtained in the invention can be observed in real time, gel electrophoresis detection on a product is not required, the risks in contaminating the PCR product are effectively reduced through completely-closed operation, and the detection speed is high; therefore, the primer-probe set and the kit are suitable for high-throughput sample detection.
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Description

technical field

[0001] The invention relates to the technical field of in vitro nucleic acid detection, in particular to a primer probe set and a kit for detecting CYP2C9 genotyping. Background technique

[0002] Cytochrome P450 2C9 (Cyt℃chrome P450 2C9, CYP2C9) accounts for 20% of the total amount of P450 in liver microsomes, and can catalyze the metabolism of many commonly used clinical drugs, and its importance in clinical drug metabolism has been paid more and more attention. CYP2C9 has a high degree of genetic polymorphism, and the more common gene mutants are CYP2C9*2 (rs1799853) and CYP209*3 (rs1057910), whose encoded enzyme activities are respectively 30% and 80% lower than wild-type CYP2C9*1, resulting in Individuals with CYP2C9 mutations require lower doses of warfarin. Individuals carrying these two alleles need a longer time to reach the steady-state concentration after taking warfarin, and have a higher risk of bleeding in the initial stage of use. Therefore, w...

Claims

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