Primer set for amplification of CYP2C9 gene, reagent for amplification of CYP2C9 gene comprising the same, and use of the same

A gene amplification and primer pair technology, applied in the field of reagents for CYP2C9 gene amplification, can solve the problems of reduced reliability of analysis results, inability to analyze a large number of samples, coding gene amplification, etc., and achieve rapid and simple amplification Reaction, rapid and simple analysis, shortening effect of amplification reaction
CN101443448AInactive Publication Date: 2009-05-27ARKRAY INC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ARKRAY INC
Publication Date
2009-05-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

Disclosed is a primer set for amplifying a region in CYP2C9 gene that contains a detection target site having CYP2C9*3 therein by a gene amplification method, which enables to amplify the region specifically. A pair of primer set is used, which comprises a forward primer comprising a nucleotide sequence depicted in SEQ ID NO:4 and a reverse primer comprising a nucleotide sequence depicted in SEQ ID NO:17. The primer set enables to amplify a region containing a site having a polymorphism CYP2C9*3 occurring in CYP2C9 gene in a specific manner and with a high degree of efficiency.
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Description

technical field

[0001] The present invention relates to a pair of primers for amplifying CYP2C9 gene, a reagent for amplifying CYP2C9 gene containing the pair of primers and an application thereof. Background technique

[0002] Cytochrome P450 is an enzyme classified into a superfamily, and there are multiple subfamilies (for example, CYP1A, CYP1B, CYP2C, CYP2D, CYP2E, CYP3A, etc.). Among them, it has been elucidated that the mutation of the gene encoding the isoenzyme CYP2C9 of the human CYP2C subfamily (CYP2C9 gene) has an effect on the disposition and influence of the substrate drugs of CYP2C9-phenytoin (antiepileptic agent) and warfarin (anticoagulant drug). The effect has an impact. The polymorphism CYP2C9*3 of the CYP2C9 gene is known to be a mutation from isoleucine to leucine at amino acid position 359 (exon 7) (Non-Patent Document 1). In addition, the 359th amino acid position of CYP2C9*3 is the recognition site of the substrate drug, and changes in the amino acid...

Claims

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