Gene detection kit for administration of angiotensin II receptor inhibitors as well as detection method and application of gene detection kit

Abstract

The invention discloses a gene detection kit for administration of angiotensin II receptor inhibitors. Specific amplification primers and sequencing primers are designed for the polymorphic angiotensin II receptor inhibitors of two genes CYP2C9 * 3 and AGTR1 (A1166C); and the kit comprises the following components of an amplification reaction solution, a CYP2C9 * 3 sequencing primer, an AGTR1 (A1166C) sequencing primer and a positive control. According to the kit, gene polymorphism related to administration of the angiotensin II receptor inhibitors is detected by using a combination of asymmetric multiplex PCR amplification and an optimized pyrosequencing technology, the kit can simultaneously detect gene polymorphism of the CYP2C9 * 3 and the AGTR1 (A1166C), the sequencing primer is a compound of carboxyl modifier agarose gel and an amino-labeled DNA sequence, the kit can be used as a sequencing primer and a capture probe, the gene polymorphism can be rapidly and accurately detected, a result is conveniently and clearly interpreted, the administration of the angiotensin II receptor inhibitors can be guided from the gene level, and a gene-oriented suggestion can be provided for clinical personalized administration.

Description

technical field [0001] The invention relates to a gene detection kit for angiotensin II receptor inhibitor medication, a detection method and an application thereof, and belongs to the field of gene detection. Background technique [0002] Angiotensin II receptor antagonists (Angiotensin II receptor antagonists, ARB) is a new type of antihypertensive drugs, antihypertensive effect is significant, especially the unique curative effect and protective effect on cardiovascular disease, causing widespread clinical concern, the World Health Organization (WHO) It has been listed as a first-line antihypertensive drug. ARB is a class of drugs that act on the renin-angiotensin system. By blocking the angiotensin II (ANG II) receptor, it mainly blocks angiotensin II present in many tissues such as blood vessels and adrenal glands. Compared with angiotensin-converting enzyme inhibitors (ACE inhibitors), it can more fully and effectively block the adverse effects of angiotensin on vasoc...

AI Technical Summary

Problems solved by technology

[0006] Conventional pyrosequencing pretreatment operations are complex and time-consuming. Therefore, it is necessary to design and develop a pyrophosphate detection kit that can be combined with asymmetric PCR technology for rapid detection.

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