Detection kit for guiding warfarin dosage with high precision and detection method thereof

A detection kit and detection method technology, applied in the biological field, can solve the problems of low accuracy of the kit and long time required, and achieve the effects of reducing the risk of bleeding, stabilizing the INR value, and improving the therapeutic effect.

Inactive Publication Date: 2017-01-25
成都睿杰森生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the above-mentioned kits also have the disadvantages of low precision and long time required to reach a stable INR value

Method used

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  • Detection kit for guiding warfarin dosage with high precision and detection method thereof
  • Detection kit for guiding warfarin dosage with high precision and detection method thereof
  • Detection kit for guiding warfarin dosage with high precision and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) First collect 2ml of peripheral blood from the patient, anticoagulate with EDTA, and use SnoMag TM Blood DNA Extraction Kit extracts genomic DNA, and operates in strict accordance with the kit instructions.

[0053] (2) Perform PCR amplification on the extracted genomic DNA. The PCR reaction system is 20ul: genomic DNA 1ul, 2×EasyTaq PCR Super Mix 10ul, primer (F / R 10uMol) 0.5ul, double distilled water (ddH 2 O) 8.5ul; PCR reaction conditions are: 94°C pre-denaturation for 5min, 94°C for 30s, 60°C for 30s, 72°C for 1min, a total of 35 cycles, 72°C extension for 5min, 12°C Hold for 0min. The primer sequences are as follows:

[0054] VKORC1 F:5'-TCCAGGGTTCAAGTGGTTCTC-3'

[0055] R:5'-ATTCATGCAGGGACATCTTTGG-3'

[0056] CYP4F2 F:5'-AGTCCCGGTCATCTCCCGCCAT-3'

[0057] R: 5'-CGCCAGCCTTGGAGAGACAGACA-3'

[0058] GGCX F:5'-GCAGAACAAGAAAGCAGGCCATCA-3'

[0059] R: 5'-TCTTAGACGCCAACAAAGGCTCCA-3'

[0060] CYP2C9*2F:5'-CACTGGCTGAAAGAGCTAACAGAG-3'

[0061] R: 5'-GTGATATGG...

experiment example 1

[0082] According to the method of Example 1, 105 patients were tested, and no false positive was found, indicating that the sensitivity and specificity of the method of the present invention both reached 100%.

experiment example 2

[0084] Taking the medication method of Example 1 as the experimental group, using the method of the Chinese patent application number 201310033313.2 as the control group 1, and taking the dosage of 3 mg as the control group 2, INR-related detection was performed on 124 patients. Among them, there are 42 people in the experimental group, 44 people in the control group 1, and 38 people in the control group 2.

[0085] The INR compliance rate of the experimental group on the 3rd, 5th, and 7th day was 45.2%, 61.9%, and 83.8%; the INR compliance rate of the control group 1 on the 3rd, 5th, and 7th day was 38.3%, 50.1%, and 69.4%. The INR compliance rates of group 2 on days 3, 5, and 7 were 11.0%, 22.7%, and 46.0%.

[0086] The time for the experimental group to reach the standard for the first time was 4.10±1.22 days; the time for the control group 1 to reach the standard for the first time was 6.23±2.30 days; the time for the control group 2 to reach the standard for the first tim...

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Abstract

The invention provides a detection kit for guiding warfarin dosage with high precision. The kit comprises amplification primers containing VKORC1 (1639G/A), CYP4F2(V433M), GGCX rs11676382, CYP2C9*2, CYP2C9*3, CYP2C9*5 and CYP2CP*6, a PCR reagent and a RFLP reagent. The invention also provides a detection method of the kit. According to the invention, the optimal dosage of warfarin of a patient can be accurately predicated, so as to assist doctors in using warfarin more reasonably and safely, thus reaching the steady INR value as far as possible, reducing the risks of bleeding and thrombus in the early stage of administration, and improving the treatment effect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a detection kit for guiding the dosage of warfarin with high precision and a detection method thereof. Background technique [0002] Warfarin is currently the most widely used oral anticoagulant. It is only effective when taken orally, and its effect is slow and long-lasting. [0003] Warfarin is widely used in the prevention and treatment of venous and arterial thromboembolic diseases. People with atrial fibrillation or mechanical heart valve replacement need to take warfarin for life. Due to its narrow therapeutic window, when the same anticoagulant dose of warfarin is used, it may lead to serious complications such as bleeding or thrombosis in different individuals, which is mainly related to individual differences in dosage, which limits its clinical application. Adverse reactions caused by improper use of farin can even be life-threatening. [0004] With the devel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/106C12Q2600/156
Inventor 喻堃
Owner 成都睿杰森生物科技有限公司
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