Primer-probe combination and kit for detecting associated genotyping of warfarin medication

Abstract

The invention belongs to the technical field of biology and relates to a primer-probe combination and a kit for detecting associated genotyping of warfarin medication. The invention provides primers and probes for detecting associated genotyping of warfarin medication. The sequences of the primers are shown as SEQ ID NO.1-SEQ ID NO.6, and the sequences of the probes are shown as SEQ ID NO.7-SEQ ID NO.12. The primer-probe combination and the kit for detecting associated genotyping of warfarin medication have the advantages that development and application of the gene detection kit are guided through warfarin medication, typing of the three genes of CYP2C9*3, CYP4F2 and VKORC1 is facilitated and easily achieved, and noninvasive diagnosis is guaranteed to enable clinicians to master patient heredity conditions rapidly and accurately, decrease medical accident incidence caused by inappropriate medicine dose greatly, reduce medication side effect and lay the foundation for improvement on clinical treatment effect; since only about 2 hours is needed for detection of every sample, high detection speed is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a combination of primers and probes and a kit for warfarin medication guiding gene detection. Background technique [0002] Warfarin is a drug commonly used in clinical practice for the prevention and treatment of thromboembolic diseases. It is a kind of coumarin anticoagulant and has an anti-vitamin K effect in the body. Warfarin can inhibit the synthesis of blood coagulation factors Ⅱ, Ⅶ, Ⅸ, and Ⅹ involved in vitamin K in the liver, and the effect and maintenance time are longer after the onset of effect. It is mainly used to prevent and treat thromboembolic diseases. The dose-response (international normalized ratio) relationship of warfarin is highly variable and influenced by many factors, and overdose can lead to bleeding, so close monitoring is required. [0003] The dose-effect relationship of warfarin is affected by genetic and environmental factors. Genetic factors mainly ...

AI Technical Summary

Problems solved by technology

However, this method is complicated to operate, and when the sample size is large, it is easy to cause cross-contamination of PCR products, and it is prone to insufficient or excessive digestion, resulting in false negative or false positive results, and low reliability.

Although the specificity of fluorescent quantitative PCR and multiplex PCR methods has been improved, the principles of these two methods are still based on the principle of ordinary PCR. Factors such as the strength of fluorescent signals, primer specificity, and low-fidelity Taq enzymes can cause impact on results

The DNA sequencing method is relatively expensive and the operation process is cumbersome. In today's rapid development of the human post-genome era, none of these methods can meet the current requirements for rapid and accurate analysis of genetic polymorphisms related to warfarin medication.

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