MiRNA landmark for lung cancer tissue typing and application thereof

A tissue typing and marker technology, applied in the field of miRNA markers, can solve the problems of unavoidable subjectivity and many steps, and achieve the effect of improving the clinical treatment effect.

Active Publication Date: 2015-06-24
常州杰傲病理诊断技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has many steps, it needs to wait for one or two days in vain, and it is unavoidable for pathology professionals to interpret the slices subjectivity

Method used

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  • MiRNA landmark for lung cancer tissue typing and application thereof
  • MiRNA landmark for lung cancer tissue typing and application thereof
  • MiRNA landmark for lung cancer tissue typing and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0016] 1. Research object

[0017] The case group consisted of 42 cases collected in Changzhou Second People's Hospital from November 2012 to March 2014. All of them were confirmed by histopathology and had not undergone surgery, radiotherapy and chemotherapy before blood collection. Among them, there were 27 cases of lung adenocarcinoma and 15 cases of lung squamous cell carcinoma; frequency matching was carried out with cases according to sex and age (±5 years old). The samples used for the research were collected at the same period, and the sampling, sub-packaging, and storage conditions were uniform, and the sample data was sorted out.

[0018] 2. Research methods

[0019] (1) Tumor tissues were embedded in paraffin and sectioned.

[0020] (2) Total RNA was extracted from paraffin tissue according to the instructions of the Paraffin Tissue Total RNA Extraction Kit (miRNeasy FFPE Kit, Cat. No. 217504, Qiagen, Germany).

[0021] (3) The reverse transcription reaction was ...

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Abstract

The invention belongs to the technical field of biology and relates to a miRNA landmark for lung cancer tissue typing and an application thereof. The miRNA landmark for lung cancer tissue typing provided by the invention comprises a miRNA-205, a miRNA-21 and a miRNA-26b. The invention also discloses primer probes with the sequences shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, and an application thereof in preparation of a lung cancer tissue typing kit. The miRNA landmark has the beneficial effects that a biomarker with very high diagnostic value (AUC is 1.000, sensitivity is 100% and specificity is 100%) for the lung cancer tissue typing is discovered for the first time and a kit capable of detecting the content of the biomarker in the tissue is provided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a miRNA marker for tissue typing of lung cancer and its application. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world, and it has become the first cause of death from malignant tumors in urban population in my country. Non-small cell lung cancer includes squamous cell carcinoma (squamous cell carcinoma), adenocarcinoma, and large cell carcinoma. Compared with small cell carcinoma, the growth and division of cancer cells are slower, and the spread and metastasis are relatively late. Non-small cell lung cancer accounts for about all lung cancers. 80% of the patients, and about 75% of the patients were found in the advanced stage, and the 5-year survival rate was very low. Among them, lung adenocarcinoma and lung squamous cell carcinoma are the two most common tumors in non-small cell lung cancer. Different pathological types of lung canc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 卢正斌
Owner 常州杰傲病理诊断技术有限公司
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