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MiRNA marker for detecting non-alcoholic fatty liver, kit, application and detection method

A non-alcoholic and marker technology, applied in biochemical equipment and methods, DNA/RNA fragments, recombinant DNA technology, etc., can solve problems that do not meet the trend of precision medicine, patients are difficult to accept, and the degree of variation is high

Pending Publication Date: 2019-07-26
成都仕康美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there are still great limitations in the detection method of fatty liver in the physical examination items, and it does not meet the trend of precision medicine.
Imaging examination and needle biopsy cannot make a diagnosis in the early stage and delay treatment; needle biopsy is invasive, the representativeness of sampling is poor, the degree of variation is high, it may lead to serious complications, and the patient's body and mind are unacceptable, etc.
In addition, although the emerging transient elastography technology in recent years can measure liver stiffness, it is still only suitable for the diagnosis of liver fibrosis in the middle and late stages

Method used

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  • MiRNA marker for detecting non-alcoholic fatty liver, kit, application and detection method
  • MiRNA marker for detecting non-alcoholic fatty liver, kit, application and detection method
  • MiRNA marker for detecting non-alcoholic fatty liver, kit, application and detection method

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Experimental program
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Effect test

Embodiment 1

[0134] A diagnostic kit for non-alcoholic fatty liver, the kit is used to detect the content of microRNA markers in plasma; the kit includes: reverse transcription primers and detection primers of the microRNA markers;

[0135] The marker is miR-122-5p;

[0136] The reverse transcription primer is: the reverse transcription primer sequence (5'-3') of miR-122-5p is shown in SEQ ID NO: 1; the detection primer is: the forward primer of miR-122-5p The sequence (5'-3') is shown in SEQ ID NO:11, and the Tm value is 60.8; the reverse primer sequence (5'-3') of miR-122-5p is shown in SEQ ID NO:12, and the Tm value for 60.

Embodiment 2

[0138] A diagnostic kit for non-alcoholic fatty liver, the kit is used to detect the content of microRNA markers in plasma; the kit includes: reverse transcription primers and detection primers of the microRNA markers;

[0139] The marker is miR-21-5p;

[0140] The reverse transcription primer is: the reverse transcription primer sequence (5'-3') of miR-21-5p is shown in SEQ ID NO: 2; the detection primer is: the forward primer of miR-21-5p The sequence (5'-3') is shown in SEQ ID NO:13, and the Tm value is 61.2; the reverse primer sequence (5'-3') of miR-21-5p is shown in SEQ ID NO:14, and the Tm value is 61.2.

Embodiment 3

[0142] A diagnostic kit for non-alcoholic fatty liver, the kit is used to detect the content of microRNA markers in plasma; the kit includes: reverse transcription primers and detection primers of the microRNA markers;

[0143] The marker is miR-192-5p;

[0144] The reverse transcription primer is: the reverse transcription primer sequence (5'-3') of miR-192-5p is shown in SEQ ID NO:3; the detection primer is: the forward primer of miR-192-5p The sequence (5'-3') is shown in SEQ ID NO:15, and the Tm value is 60.3; the reverse primer sequence (5'-3') of miR-192-5p is shown in SEQ ID NO:16, and the Tm value was 61.7.

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Abstract

The invention discloses a miRNA marker for detecting non-alcoholic fatty liver, a kit, an application and a detection method, wherein the miRNA marker is any one or more of miR-122-5p, miR-21-5p, miR-192-5p, miR-223-3p, miR-29a-3p, miR-331-3p, miR-30c-5p, miR-182-5p, miR-224-5p and miR-221-5p; the marker and kit can be used for assistant diagnosis of non-alcoholic fatty liver, and are easy to detect, accurate in quantification, and good in sensitivity and specificity.

Description

technical field [0001] The invention relates to the technical field, in particular to a miRNA marker, kit, application and detection method for detecting non-alcoholic fatty liver. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is a metabolic stress-induced liver injury without a history of excessive drinking, closely related to insulin resistance (insulin resistance, IR) and genetic susceptibility (geneticsusceptibility). Alcoholic liver steatosis (non-alcoholichepatic steatosis), non-alcoholic steatohepatitis (non-alcoholic steatohepatitis, NASH), liver cirrhosis and hepatocellular carcinoma (hepatocellularcarcinoma, HCC). NAFLD can not only lead to liver disease disability and death, but also closely related to the high incidence of metabolic syndrome (MetS), type 2 diabetes (type2diabetes mellitus, T2DM), atherosclerotic cardiovascular disease and colorectal tumors. With the global prevalence of obesity and related metabolic syndrome, non-alcoho...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6851C12N15/113C12N15/11
CPCC12Q1/6883C12Q1/6851C12Q2600/158C12Q2600/178C12Q2563/159C12Q2525/207C12Q2521/107Y02A50/30
Inventor 魏亮
Owner 成都仕康美生物科技有限公司
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