Gene chip for screening various ophthalmological hereditary diseases as well as preparation and usage method of gene chip

Abstract

The invention relates to the technical field of biological gene chips, and particularly relates to a gene chip for screening various ophthalmological hereditary diseases as well as preparation and a usage method of the gene chip. Specific oligonucleotide probes of gene sequences which are related to 261 ophthalmological hereditary diseases are fixed on the surface of a carrier of the gene chip for screening various ophthalmological hereditary diseases; and the gene chip which is related to the 261 ophthalmological hereditary diseases comprises the sequences of all coding region sequences of 954 related virulence genes or disease predisposing genes and introne sequences adjacent to the coding regions. As the specific oligonucleotide probes of the gene sequences which are related to 261 ophthalmological hereditary diseases and the sequences of all the coding region sequences of the related virulence genes or disease predisposing genes and the introne sequences adjacent to the coding regions are fixed on the surface of the carrier of the gene chip, the gene chip provided by the invention is capable of screening a plurality of ophthalmological hereditary diseases at the same time and then the efficiency is greatly improved.

Description

technical field [0001] The invention relates to the technical field of biological gene chips, in particular to a gene chip for screening various ophthalmic hereditary diseases and methods for preparing and using the same. Background technique [0002] More than 200 ophthalmic genetic diseases have been reported so far. According to different anatomical locations, it can be divided into lacrimal disease, eyelid disease, corneal disease, glaucoma, lens disease, pigmented membrane disease, retinopathy, vitreous body disease, optic neuropathy, ophthalmopathy, ocular refractive error and syndrome, etc.; Different ways of inheritance can be divided into autosomal recessive inheritance, autosomal dominant inheritance, sex chromosome linkage inheritance and mitochondrial inheritance; according to the number of genes, it can be divided into monogenic inheritance and polygenic inheritance. Congenital glaucoma, retinitis pigmentosa, Leber hereditary optic neuropathy, Usher syndrome a...

AI Technical Summary

Problems solved by technology

[0007] As for the first problem, it is difficult to screen candidate genes, and there is still a lack of solutions

In the current field of ophthalmic genetics, there are only a few known gene diagnostic chips for Leber hereditary optic neuropathy. Since they do not contain the entire sequence of the mitochondrial genome, only a part of the patients with Leber hereditary optic neuropathy can be detected

For other more than 200 complex genetic eye diseases including retinitis pigmentosa, there is not even any genetic diagnosis chip

The main reason is that although the eye is a small organ, it has a complex structure and fine functions, and there are many departments in ophthalmology. Combine strong knowledge of genetics and bioinformatics

Therefore, in the current field of ophthalmology, there is a lack of a gene chip containing various ophthalmic genetic diseases to provide a clear clinical diagnosis, and then provide genetic counseling for patients and lay the foundation for subsequent treatment

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