Detection technology of molecular beacon strand displacement isothermal amplifying gene chip and kit

Abstract

The invention discloses a detection technology of molecular beacon strand displacement isothermal amplifying gene chip and a kit. The detection technology disclosed by the invention combines the advantages of strand displacement isothermal amplifying technology and molecular beacon technology. According to the detection technology, DNA (Deoxyribose Nucleic Acid) is amplified under the isothermal condition; fluorescence signals of the molecular beacon are continuously released along with the amplification of the DNA, and the released target DNA are combined to new molecular beacon, so that the amplification and releasing of the fluorescence signals are carried out in turn. According to the detection technology, the purpose of amplifying the signals is achieved based on the strand displacement reaction of DNA polymerase; and the purpose of detecting signals is achieved based on open loop and closed loop of the molecular beacon. With the adoption of the detection technology disclosed by the invention, the amplification of the DNA / RNA (Ribose Nucleic Acid) and the detection of signals are integrated, the processes such as additional marketing, hybridizing and washing are saved, so that, the operation steps are simplified, the efficiency is improved; and moreover, the chip can be detected on real time, and accurate and reliable results are obtained.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular beacon strand displacement isothermal amplification gene chip detection technology. Background technique [0002] Gene chips are also called DNA chips, DNA microarrays, and oligonucleotide arrays. This technology refers to immobilizing a large number of probe molecules on a support and then hybridizing with labeled sample molecules. The hybridization signal intensity of each probe molecule is used to obtain the number and sequence information of the sample molecules to achieve rapid, parallel, and efficient detection or medical diagnosis of biological samples. Since silicon chips are commonly used as solid supports and used in the preparation process The preparation technology of the computer chip, so it is called the gene chip technology. [0003] Because gene chip technology immobilizes a large number of probes on the support at the same time, it can detect...

AI Technical Summary

Problems solved by technology

In terms of sample preparation, most companies currently have to amplify the sample to a certain extent before labeling and measurement in order to improve the sensitivity of detection, but there are still many people trying to bypass this problem, including Mosaic Technologies' solid-phase PCR Amplification system and a large number of parallel solid-phase cloning methods proposed by Lynx Therapeutics, both methods have their own advantages and disadvantages, but have not yet been practically applied

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