Primer sequence for cauliflower mosaic virus 35s promoter-containing transgenic crop nucleic acid amplification

A cauliflower flower leaf and primer sequence technology, applied to the determination/testing of microorganisms, biochemical equipment and methods, etc.
CN1470645AInactive Publication Date: 2004-01-28QIAGEN SHENZHEN CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
QIAGEN SHENZHEN CO LTD
Publication Date
2004-01-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides the primar sequences for nucleic acid amplification of the transgenic crop containing caulimovirus 35s promotor, they are contained in the extended regional range of four pairs of optimal primer sequences, and the extended regional range of every pair of primer sequence is: the primer pair is formed from upstream primer and downstream primer, in the upstream primer position of said primer pair 10 bases are forward extended and 10 bases are backward extended, and in the downstream primer position 10 bases are forward extended and 10 bases are backward extended, in the regional range of upstream and downstream extended positions of said primer pair the primer sequence can be obtained.
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Description

technical field

[0001] The invention relates to a primer sequence for nucleic acid amplification of transgenic crops containing cauliflower mosaic virus 35s promoter Background technique

[0002] In the past three to four years, the detection of genetically modified products has become a major event at home and abroad, which has attracted widespread attention and attention from all walks of life. Although agencies affiliated to the United Nations (FAO, WHO, etc.) and some regional international organizations are convening to establish a unified worldwide testing technology and method standard, due to inconsistencies in the safety of genetically modified products, risk management measures and other interests, countries currently It is still difficult to reach a consensus. Based on the detection technology of GMO products in various countries in the world, it is mainly divided into the following three types according to the detection target: detection of inserted foreign gene...

Claims

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