Grape VyLhcb4 gene as well as encoding protein and application thereof in breeding stress-resistant variety

A technology for encoding proteins and grapes, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of few species, the biochemical characteristics of gene structure encoded proteins, the evolutionary relationship and the lack of research reports on stress response, etc., and achieve stress resistance enhanced effect

Active Publication Date: 2020-05-15
HENAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

As one of the most important functional proteins in PSI, the current research on LHCI protein mainly focuses on the capture and transmission of light energy, involvin

Method used

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  • Grape VyLhcb4 gene as well as encoding protein and application thereof in breeding stress-resistant variety
  • Grape VyLhcb4 gene as well as encoding protein and application thereof in breeding stress-resistant variety
  • Grape VyLhcb4 gene as well as encoding protein and application thereof in breeding stress-resistant variety

Examples

Experimental program
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Embodiment 1

[0015] Example 1 Grape VyLhcb4 gene expression analysis

[0016] After subculture of Yanshan grape tissue culture seedlings for 16 days, the seedlings with consistent growth and performance were selected for various stress treatments.

[0017] Drought treatment: Pull out the grape seedlings from the culture medium, place them on filter paper and expose them to a room temperature of (32±1)°C, a relative humidity of 55%, and a photoperiod of light 14h / darkness 10h. 2, 6, 12, 24h sampling.

[0018] Low temperature treatment: culture the tissue cultured seedlings at a temperature of (4±1)°C, a relative humidity of 75%, and a photoperiod of 14 hours of light / 10 hours of darkness, and take samples at 0, 2, 6, 12, and 24 hours.

[0019] Salt stress: add 20mL 100mmol·L -1 The NaCl solution was cultivated at a temperature of (25±1)°C, a relative humidity of 75%, and a photoperiod of 14 h light / 10 h dark, and samples were taken at 0, 2, 6, 12, and 24 h.

[0020] An equal volume of di...

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Abstract

The invention relates to a grape VyLhcb4 gene as well as an encoding protein and application thereof in breeding a stress-resistant variety and belongs to the technical field of plant gene engineering. By adopting a transgenic technology of a strong promoter (cauliflower mosaic virus promoter 35S), an over-expression vector of a Yanshan grape VyLhcb4 gene is transferred into arabidopsis thaliana,so that a transgenic arabidopsis thaliana plant is obtained. Experiments show that compared with an arabidopsis thaliana plant with a transferred empty vector, the transgenic arabidopsis thaliana plant has improved stress resistance since related stress-resistant substances are accumulated and a related stress-resistant gene is expressed in transgenic arabidopsis thaliana since the VyLhcb4 gene isover-expressed. Therefore, the grape VyLhcb4 gene and a recombinant expression vector thereof can be applied to breeding of stress-resistant plant varieties.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to the grape VyLhcb4 gene and its encoded protein and the application of the gene in stress-resistant variety breeding. Background technique [0002] Light-harvesting chlorophyll a / b-binding protein is an important functional protein in the photosynthetic system. It is located on the thylakoid membrane of chloroplasts and combines with pigments to form a light-harvesting pigment protein complex. The complex can capture light energy, and quickly transmit the light energy to the reaction center of PSI and photosystem II, causing photochemical reaction, converting light energy into chemical energy. In higher plants and green algae, the PSI complex consists of two parts, the PSI core complex and the light-harvesting pigment protein complex I. The PSI complex of eukaryotes includes a series of light-harvesting chlorophyll a / b-binding proteins (LHCI), which bind about 1...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/20A01H6/88
CPCC07K14/415C12N15/8273
Inventor 余义和刘风英张海涛李敏张贺程郭大龙杨英军张国海
Owner HENAN UNIV OF SCI & TECH
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