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InDel molecular marker closely linked with epistatic gene P of eggplant fruit color and application of InDel molecular marker

A molecular marker, 1.indel technology, applied in the field of molecular biology and molecular genetics, can solve the problems of undeveloped molecular marker research and unclear positioning genes

Active Publication Date: 2018-05-15
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0004] At present, the chromosomal location of the eggplant fruit color epistasis gene locus and the specific gene are not yet clear, and no research on related molecular markers has been carried out.

Method used

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  • InDel molecular marker closely linked with epistatic gene P of eggplant fruit color and application of InDel molecular marker
  • InDel molecular marker closely linked with epistatic gene P of eggplant fruit color and application of InDel molecular marker
  • InDel molecular marker closely linked with epistatic gene P of eggplant fruit color and application of InDel molecular marker

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Embodiment Construction

[0026] In the existing references (Tigchelaar E C, Janick J, Erichson H T. The genetics of anthocyanin coloration in eggplant (Solanum melongena L.). Genetics, 1968, 60:475-491), the epistatic gene locus controlling eggplant fruit color They are respectively defined as D gene and P gene. This study continues to use the name of the gene according to the phenotypic characteristics and genetic rules of the parental materials.

[0027] The present invention will be further described below in conjunction with experiments, but it is not limited thereto.

[0028] The molecular biology experimental techniques adopted in the following examples include experiments such as DNA extraction, PCR amplification, and PAGE gel electrophoresis. If there is no special instruction, they are usually operated according to conventional methods. For details, please refer to "Molecular Cloning Experiment Guide" (Third Edition) (Sambrook J, Russell DW, Janssen K, Argentine J. Huang Peitang etc. translat...

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Abstract

The invention discloses an InDel molecular marker closely linked with an epistatic gene P of eggplant fruit color and application of the InDel molecular marker. The genetic distance between the InDelmolecular marker and the epistatic gene P is 0.5cM, and InDel of specific insertion / deletion fragments at 50bp and 35bp in nucleotide sequences of the male parent is not found from the female parent.The InDel molecular marker closely linked with the epistatic gene P of the eggplant fruit color, disclosed by the invention, lays a foundation for fine mapping and cloning of the epistatic gene of theeggplant fruit color. An InDel 5513 molecular marker and a molecular marker amplification primer can be simply and rapidly applied in eggplant fruit color breeding practice at high flux, so that theprogress of improving fruit color characters of eggplants is accelerated.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to the field of molecular genetics, in particular to an InDel molecular marker closely linked with eggplant fruit color epistasis gene P and its application. Background technique [0002] The fruit color of eggplant directly affects the appearance of eggplant, and is an important commodity appearance quality of the fruit. The fruit color of eggplant is rich, including purple black, purple red, green and white. Due to different consumption habits, different regions have different preferences for eggplant fruit color. Therefore, eggplant fruit color is an important breeding goal. [0003] Inheritance of eggplant fruit color is a complex trait controlled by multiple genes. Pang Wenlong, Liu Fuzhong, Chen Yuhui, et al. Genetic research on fruit color traits of eggplant [J]. Acta Horticultural Science, 2008, 35(7): 979-986. Three inbred lines of eggplant cultivars with different fruit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 孙保娟李植良黎振兴罗少波吴俊
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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