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InDel marker of cucumber anti-cucumber-mosaic-virus-disease gene cmv and its application

A technology of cucumber mosaic virus and indel-cmv3-r, which is applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., and can solve problems such as no other types of markers.

Active Publication Date: 2018-02-09
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the currently reported markers linked to resistance to cucumber mosaic virus disease are only SSR markers, and there are no studies on other types of markers (such as InDel, SNP, etc.)

Method used

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  • InDel marker of cucumber anti-cucumber-mosaic-virus-disease gene cmv and its application
  • InDel marker of cucumber anti-cucumber-mosaic-virus-disease gene cmv and its application
  • InDel marker of cucumber anti-cucumber-mosaic-virus-disease gene cmv and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1. Acquisition of the InDel marker InDel-cmv3 of cucumber anti-cucumber mosaic virus gene cmv

[0038] Step 1. Identification of Cucumber Mosaic Virus Resistance in Cucumber

[0039] In this experiment, high-generation cucumber inbred lines '65G' (susceptible) and '02245' (disease-resistant) were used as female and male parents, and F 1 Populations, RIL populations were obtained by single-seed method and DH populations were obtained by megaspore culture.

[0040] In the autumn of 2016, the cultivation experiment was carried out in the glass greenhouse of the North Field of the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences. A completely randomized block design was adopted, and the parents 65G, 02245, F 1 , RIL population, and DH population were planted in 21-hole plug trays, repeated 3 times, with 7 plants in each repetition, and were routinely cultivated and managed in the field. When the first true leaf of cucumber...

Embodiment 2

[0081]Example 2. Verification of the InDel-cmv3 marker linked to the cucumber cmv gene

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Abstract

The invention provides an InDel marker of a cucumber anti-cucumber-mosaic-virus-disease gene cmv and its application, belonging to the field of biotechnological assistant breeding. The nucleotide sequence of a primer InDel-cmv3-F / InDel-cmv3-R for the InDel marker closely linked with the cucumber anti-cucumber-mosaic-virus-disease gene cmv is as described in the specification; a characteristic band amplified by the InDel marker and closely linked with the anti-cucumber-mosaic-virus-disease gene cmv has a length of 223 bp; and a characteristic band amplified by the InDel marker and closely linked with a cucumber-mosaic-virus-disease susceptibility gene CMV has a length of 245bp. The marker provided by the invention has the advantages of high efficiency and little limitation, improves the efficiency of breeding of anti-cucumber-mosaic-virus-disease cucumber materials and shortens a breeding cycle.

Description

technical field [0001] The invention relates to the technical field of biotechnology-assisted breeding, in particular to an InDel marker of the cucumber mosaic virus resistance gene cmv and its application in the selection of cucumber breeding materials. Background technique [0002] Cucumber is one of the important vegetable crops. Virus diseases are the main diseases affecting cucumber production. Cucumber mosaic virus (CMV) is a major pathogen that causes virus diseases. After the cucumber is infected by virus disease, there will be bright veins on the new leaves in the early stage, and there will be irregular light green or yellow spots on the leaves in the later stage, that is, mosaic symptoms, the green part is extremely uneven, the leaf margins turn outwards, and the leaves become narrow , There are blister spots on the new leaves, and when it is severe, the leaves are shrunken and deformed, and the plants are dwarfed until they die. After the fruit is infected, ther...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 顾兴芳张圣平苗晗史利雪王烨
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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