Method for developing waxy1-gene internal molecular markers on basis of association analysis and KASP

A technology of association analysis and molecular markers, applied in the biological field, can solve problems such as low selection efficiency, unfavorable high-throughput screening, and complicated operations

Active Publication Date: 2018-01-23
SHANGHAI JIAO TONG UNIV
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  • Abstract
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Problems solved by technology

[0005] The object of the present invention is to provide a molecular marker based on association analysis and KASP technology to develop waxy1 gene in

Method used

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  • Method for developing waxy1-gene internal molecular markers on basis of association analysis and KASP
  • Method for developing waxy1-gene internal molecular markers on basis of association analysis and KASP
  • Method for developing waxy1-gene internal molecular markers on basis of association analysis and KASP

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Embodiment

[0053] This embodiment involves the development of molecular markers in the waxy1 gene based on genome-wide association analysis and KASP technology; the specific content is as follows:

[0054] 1. Materials and methods:

[0055] 1.1 Plant material

[0056] The present invention collects 465 temperate inbred lines from China Agricultural University, 38 high-quality protein corn inbred lines from Yunnan Academy of Agricultural Sciences and 5 sticky waxy lines purchased from Amazon (sold by Shenzhen Feidu Times Network Co., Ltd.) Maize hybrids (purple sticky corn, color sticky corn, white sticky corn, white waxy corn, golden waxy corn) and all other corn genetic materials come from our laboratory. Except for 5 waxy maize hybrids, all other genetic materials were sown and propagated in Sanya, Hainan in November 2013.

[0057] 1.2 Determination of apparent amylose content (AAC) of corn kernels

[0058] Preparation of standard curve: Weigh 100 mg of potato amylose standard sampl...

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Abstract

The invention discloses a method for developing waxy1-gene internal molecule markers on the basis of association analysis and KASP. One indel and two snap markers located in waxy1 gene and remarkablyassociated with content of amylase are found out mainly through genome-wide association study and candidate gene association analysis techniques; the detected indel is marked as a major mutant type ofglutinous maize in China; glutinous maize materials can be identified through agarose gel by the markers developed on the basis of the indel, and convenience and rapidness is achieved as compared with that of original polyacrylamide gel identification; meanwhile, paired linkage disequilibrium test of 59 parts of waxy1-gene sequence regions of maize hybrid line materials is performed, close linkage of the indel marker and the two snp markers is tested, primers of the two snps are developed on the basis of the KASP technology, the glutinous maize materials can be identified rapidly, efficientlyand accurately, and molecular breeding of glutinous maize is facilitated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the development of molecular markers in the waxy1 gene based on genome-wide association analysis and KASP technology. Background technique [0002] Maize is one of the main crops widely grown in many countries and is an important food, economic and feed crop. Modern maize is evolved from wild maize after nearly 10,000 years of history, during which unique geographical strains and mutant strains all over the world have been formed. The differences in specific traits between these lines and wild maize and within the lines are essentially caused by the accumulation of mutations at certain sites on the genome under long-term artificial directional selection. These loci usually control important agronomic traits and are important gene loci that need to be screened in breeding. Therefore, how to efficiently identify, utilize and aggregate these loci is a problem and challenge...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 王文琴李长生巫永睿
Owner SHANGHAI JIAO TONG UNIV
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