Method for culturing Metarhizium anisopliae
A technology of Metarhizium anisopliae and culture medium, which is applied in the field of cultivating Metarhizium anisopliae, can solve the problems of hindering the production process of Metarhizium anisopliae, low spore content of the bacterial agent, and long production cycle, so as to reduce the cultivation time and use Space, high production efficiency, economical and practical production process
Examples
Embodiment 1
[0027] 1. First-level plate culture:
[0028] Inoculate the isolated and purified strains on a PDA medium plate, put them in a 28°C incubator, and cultivate them for 7 to 10 days. When the mycelia are all over the plates and produce spores, transfer them to shake flasks for culture;
[0029] The composition of each 1000ml PDA medium: potato 200g, peptone 10g, glucose 20g, agar 20g, water 1000ml.
[0030] 2. Liquid seed culture:
[0031] In a 1000ml triangular flask, put 400ml of liquid seed culture medium into it, insert the plate bacteria, and the inoculation amount is 1000ml triangular flask to connect 1 / 6 of the well-cultured first-level plate bacteria (petri dish specification 90mm), after inoculation, place Cultivate in a constant temperature shaker (28°C, 180 rpm) for 3 to 5 days;
[0032] The composition of each 1000ml liquid seed medium: 20g corn flour, 40g bean cake powder, 2.5g peptone, 0.5g KH 2 PO 4 , 0.5gMgSO 4 , 0.5gKCl, 0.01gFeSO 4 ·7H 2 O and 1000ml of w...
Embodiment 2
[0050] 1. Strain cultivation on a first-level flat plate: the same as the method described in Example 1.
[0051] 2. Liquid seed culture: the same as the method described in Example 1.
[0052] 3. Liquid fermentation production: the method is the same as that described in Example 1, except that the cultivation time is 68 hours.
[0053] 4. Solid fermentation production
[0054] The solid fermentation method is the same as the method described in Example 1, except that the cultivation time of the a stage is 3 days, and the solid culture material is composed differently, as follows:
[0055] Composition of solid compost: bagasse, wheat bran, corn flour, KH 2 PO 4 , MgSO 4 , KCl and water; its mass and number ratio is: 350 parts of bagasse: 180 parts of bran: 120 parts of corn flour: 0.5 part of KH 2 PO 4 : 0.5 parts of MgSO 4 : 0.5 parts of KCl: 350 parts of water; natural pH, steam sterilization at 121°C for 60 minutes;
[0056] 5. Drying of fermentation products
[00...
Embodiment 3
[0060] 1. Strain cultivation on a first-level flat plate: the same as the method described in Example 1.
[0061] 2. Liquid seed culture: the same as the method described in Example 1.
[0062] 3. Liquid fermentation production: the same method as described in Example 1, except that the cultivation time was 72 hours.
[0063] 4. Solid fermentation production
[0064] The solid fermentation method is the same as the method described in Example 1, except that the solid compost composition is different, as follows:
[0065] Composition of solid compost: bagasse, wheat bran, corn flour, KH 2 PO 4 , MgSO 4 , KCl and water; its mass and number ratio is: 400 parts of bagasse: 200 parts of bran: 90 parts of corn flour: 0.5 part of KH 2 PO 4 : 0.5 parts of MgSO 4 : 0.5 parts of KCl: 400 parts of water; natural pH, steam sterilization at 121°C for 60 minutes;
[0066] 5. Drying of fermentation products
[0067] The method is the same as that described in Example 1.
[0068] Re...
PUM
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Description
Claims
Application Information
- IPC
- C12N1/14; C12R1/645
- Inventors
- 黄俊生; 梁昌聪