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Compounding pesticide containing Beauveria bassiana and destruxins of Metarrhizium anisopliae and preparation method thereof

A technology of Beauveria bassiana and Beauveria anisopliae, which is applied in the directions of botanical equipment and methods, biocides, biocides, etc., can solve problems such as unsatisfactory control effect, insufficient virulence, and no technical reports, etc. To achieve the effect of significant insecticidal effect, simple preparation method and environmental friendliness

Inactive Publication Date: 2009-05-27
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when metarhizolin is used alone, the toxicity is not high enough, and the control effect is not ideal
[0005] At the same time, long-term use of the same insecticide is likely to cause the development of pest resistance, which also puts forward new development requirements for mixed pesticides. Continuous development and research of pesticide compounding technology or mixed application technology is an important way to overcome or delay pesticide resistance. method
[0006] In summary, the applicant intends to use the synergistic effect of metarhizium on entomogenic fungi to develop a mixed insecticide for Beauveria bassiana and metarhizium, especially for whitefly , there is currently no technical report on related technologies

Method used

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  • Compounding pesticide containing Beauveria bassiana and destruxins of Metarrhizium anisopliae and preparation method thereof
  • Compounding pesticide containing Beauveria bassiana and destruxins of Metarrhizium anisopliae and preparation method thereof
  • Compounding pesticide containing Beauveria bassiana and destruxins of Metarrhizium anisopliae and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the preparation of synergistic insecticide of the present invention

[0025] (1) Get the conidia of Beauveria bassiana coccidioidum, and use 0.01~0.05% Tween 80 solution to prepare a concentration of 10 10～12 live conidia / L of spore suspension;

[0026] (2) Get metarhizine, and use solvents such as acetone or ethyl acetate to prepare a stock solution whose active ingredient concentration is greater than 100000mg / L;

[0027] (3) The conidia suspension described in the above steps (1) is mixed with the metrhizine stock solution described in the step (2) in a certain proportion, so that the final concentration remains as live conidia 10 9～11 Individual / L, metarhizine 10-100mg / L.

Embodiment 2

[0028] Embodiment 2 Indoor toxicity determination experiment of synergistic insecticide of the present invention to agricultural and forestry pests

[0029] The present embodiment takes Bemisia tabaci (Bemisia tabaci) as the main experimental object, and when carrying out indoor virulence measurement, use hibiscus rosa-sinensis L as the test plant, its potted seedlings are raised in insect cages, and the whitefly bemisia tabaci Insert the adults into the insect cages, drive them out after one day of laying eggs, keep the temperature of 26°C and the light of 14:10 in the cages, and when the Bemisia tabaci on the leaves of Hibiscus tabaci grow to the 2nd instar nymphs, dipping Toxicity test was carried out by the method, repeated 4 times, the effect of 4 days after the drug was investigated, the corrected mortality rate was calculated, the regression equation of the logarithm of the drug concentration-the probability value of the mortality rate was obtained, and the chi-square su...

Embodiment 3

[0038] Embodiment 3 Field efficacy test of insecticides according to the present invention to Bemisia tabaci

[0039] The experiment was carried out on tomatoes grown in greenhouses in May 2008. The temperature was 22-30°C, the relative humidity was 81-97%, and the plot area was 30m2. 2 , randomized block design, repeated 4 times, sprayed at the peak of the 2nd instar nymphs of Bemisia tabaci, surveyed once every 2 days after spraying, fixedly inspected 10 points in each plot (each point was about 10cm 2 Leaf area), no less than 100 nymphs at each point, record the total number of insects and (disease) dead insects, calculate the corrected mortality rate, and perform analysis of variance and new multiple range test. The test results are shown in Table 2. The control effect of the synergistic insecticide of the present invention is obviously better than that of Beauveria bassiana and metrhizin alone.

[0040] Table 2 Comparison of control effects of several pesticides on Bemis...

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Abstract

The invention discloses a pesticide compounding beauveria bassiana fungus and destruxin. The pesticide is prepared by mixing the beauveria bassiana fungus, the destruxin and solvent, the concentration of live conidia of the beauveria bassiana fungus after mixing ranges from 10<7> to 10<11> conidia per liter, and the pesticidal effect is better when the concentration of the destruxin ranges from 10 mg / L to 100 mg / L. The preparation method for the pesticide comprises the following steps: preparing conidial suspension of the beauveria bassiana fungus, preparing destruxin stock solution, mixing the conidial suspension and the destruxin stock solution, and the like. The invention selects the beauveria bassiana fungus to be used with the destruxin in a mixing way for synergic action, and the pesticide has the completely new function mechanism different form the prior pesticide, has an excellent pesticidal effect particularly in poisoning and killing bemisia tabaci gennadius with serious harm, and meanwhile is environment-friendly.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and relates to a mixed insecticide of Beauveria bassiana fungus and metarhizin and a preparation method thereof. Background technique [0002] The prevention and control of important agricultural and forestry pests such as Lepidoptera, Hemiptera, Homoptera and Coleoptera is very important. For example, the whitefly Bemisia tabaci (Gennadius) belongs to the family Aleyrodidae of the order Hopoptera and is an important worldwide pest. After the 1970s, with the appearance of type B Bemisia tabaci, the harm of Bemisia tabaci to field crops, vegetables and flowers has become increasingly serious, especially in recent years, Bemisia tabaci have been breaking out in succession all over the world. Bemisia tabaci has been breaking out successively in different regions of my country since the early 1990s, especially seriously damaging crops such as cucumber, tomato, eggplant, melon and zucch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P7/00
Inventor 胡琼波任顺祥
Owner SOUTH CHINA AGRI UNIV
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