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Liquid medium for Bacillus thuringiensis, and culture fermentation method thereof

A technology of liquid culture medium and Bacillus thuringiensis, which is applied in the field of microorganisms, can solve the problems of high production process requirements, unstable use effect, and too many operations, and achieve the effects of low cost, low production and use costs, and expanded use range

Inactive Publication Date: 2018-08-24
广西青又青生物肥业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the Bacillus thuringiensis on the market are added with hidden ingredients, and the use effect is unstable. However, the pure Bacillus thuringiensis solid powder has high production process requirements and too many operations, resulting in higher costs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) inoculate the Bacillus thuringiensis species in the PDA-glucose medium under aseptic conditions to carry out slant culture, control the culture temperature to be 30° C., and cultivate for 48 hours to obtain the Bacillus thuringiensis species on the slant;

[0027] (2) inoculate the Bacillus thuringiensis slant species in step (1) in the expansion medium under aseptic conditions, place in a shaker at 100rpm and cultivate for 12h, and control the culture temperature to be 30°C to obtain the Bacillus thuringiensis seed liquid;

[0028] (3) After the liquid culture medium of Bacillus thuringiensis is sterilized, it is cooled to 39° C. and set aside;

[0029] (4) Under the pressure of 0.08Mpa, the fermenter is sterilized by steam at 115° C. for 30 minutes; put into the Bacillus thuringiensis liquid culture medium of step (3), and then add the Bacillus thuringiensis seed liquid of step (2) into Bacillus thuringiensis liquid culture medium is inoculated and cultivated and ...

Embodiment 2

[0033] (1) Inoculate the Bacillus thuringiensis species in PDA-glucose medium under aseptic conditions to carry out slant culture, control the culture temperature to be 30.2°C, and cultivate for 42h to obtain the Bacillus thuringiensis species on the slant;

[0034] (2) inoculate the Bacillus thuringiensis slant species in step (1) in the expansion medium under aseptic conditions, place it in a shaker at 120rpm and cultivate it for 14h, and control the culture temperature to be 30.5°C to obtain the Bacillus thuringiensis seed liquid;

[0035] (3) After the liquid culture medium of Bacillus thuringiensis is sterilized, it is cooled to 38° C. and set aside;

[0036](4) Under the pressure of 0.09Mpa, the fermenter was sterilized by steam at 117°C for 27 minutes; the Bacillus thuringiensis liquid culture medium of step (3) was put into it, and the Bacillus thuringiensis seed liquid of step (2) was added into Bacillus thuringiensis liquid culture medium is inoculated and cultivated...

Embodiment 3

[0040] (1) Inoculate the Bacillus thuringiensis species in PDA-glucose medium under aseptic conditions to carry out slant culture, control the culture temperature to be 30.5°C, and cultivate for 36h to obtain the Bacillus thuringiensis species on the slant;

[0041] (2) inoculate the Bacillus thuringiensis slant species in step (1) in the expansion medium under aseptic conditions, place it in a shaker at 140rpm and cultivate it for 17h, and control the culture temperature at 31°C to obtain the Bacillus thuringiensis seed liquid;

[0042] (3) After the liquid culture medium of Bacillus thuringiensis is sterilized, it is cooled to 37° C. and set aside;

[0043] (4) Sterilize the fermenter with steam at 119°C for 22min under a pressure of 0.1Mpa; put the Bacillus thuringiensis liquid culture medium in step (3), then add the Bacillus thuringiensis seed liquid in step (2) into Bacillus thuringiensis liquid culture medium is inoculated and cultivated and fermented, and the addition ...

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Abstract

The invention discloses a liquid medium for Bacillus thuringiensis. The liquid medium comprises molasses, extract yeast, urea, dipotassium hydrogen phosphate, anhydrous sodium acetate, calcium carbonate, magnesium sulfate, manganese sulfate, iron chloride, wort, corn steep liquor and sterile water. The invention also provides a method for culturing and fermenting the Bacillus thuringiensis by using the liquid medium. The liquid medium for Bacillus thuringiensis has the advantages of low cost, simplicity in operation of the culturing method, overcoming of the disadvantages of high cost, no large-scale production and complex production operation of traditional Bacillus thuringiensis media, simple components, and convenience in preparation, and the Bacillus thuringiensis cultured in the invention has the advantages of high live bacterium content reaching 2.5 * 10<9> cfu / mL, low competitor content, good stability, and convenience in long-distance transportation and long-term preservation.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and more specifically relates to a liquid culture medium and a culture and fermentation method of Bacillus thuringiensis. Background technique [0002] Bacillus thuringiensis, referred to as Bt, is a class of crystal-producing bacillus including many varieties. It can be used as a low-toxic insecticide derived from microorganisms, mainly with stomach toxicity. The bacteria can produce two types of toxins, namely endotoxin (paracellular crystals) and exotoxin, which stop the pests from feeding, and finally the pests die due to starvation and poisoning. Therefore, the bacillus can be used as a low-toxic insecticide derived from microorganisms, and can be used to prevent and control various pests of Orthoptera, Coleoptera, Diptera, Hymenoptera, especially Lepidoptera, and is safe for non-target organisms. [0003] However, exotoxin acts slowly, and its effect is obvious during molting and m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07
CPCC12N1/20
Inventor 苏善高陈文艳赵文青
Owner 广西青又青生物肥业有限公司
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