Pathogenic fungus for biological control of cockroaches

A technology of microbial strains and microbial mixtures, applied in the direction of microorganism-based methods, microorganisms, fungi, etc., can solve the problems of human and animal hazards, weak virulence, poor host selectivity, long killing time, etc., and achieve control of insect populations , strong selectivity, the effect of protecting natural enemies

Active Publication Date: 2017-03-29
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention is aimed at the extensive drug resistance, environmental pollution, harm to humans and animals and weak toxicity, low lethality, long insecticidal time, poor selectivity to hosts and whether it can Kill many problems such as German cockroaches in various growth stages, provide a ki...

Method used

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  • Pathogenic fungus for biological control of cockroaches
  • Pathogenic fungus for biological control of cockroaches
  • Pathogenic fungus for biological control of cockroaches

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Screening of Metarhizium anisopliae (Metarhizium anisopliae)

[0037] (1) Screening process

[0038] The separation and purification are all carried out under the ultra-clean workbench: use the inoculation needle to dip the surface spore powder of the German cockroach and streak it on a 90mm diameter PDA medium coated with 0.5% gentamicin, or use a sterile Grasp the zombies with forceps and gently and quickly shake them on the medium. Put it into a 26℃ constant temperature incubator and cultivate under dark conditions. After 3 days, pick the culture on the medium and transfer it to a new flat PDA medium with a diameter of 90 mm coated with gentamicin. Repeat the operation several times to obtain a pure strain. The isolated pure culture of Metarhizium anisopliae was used to recover the healthy German cockroach, and the same Metarhizium anisopliae was re-isolated from the corpse of the German cockroach that was infected with the disease, and it was determined to be...

Embodiment 2

[0044] Example 2 Determination of virulence of Metarhizium anisopliae (Metarhizium anisopliae) to German cockroach

[0045] Take the conidia of Metarhizium anisopliae on the PDA medium and perform the concentration gradient bioassay, and prepare the spores of Metarhizium anisopliae into 1×10. 5 , 1×10 6 , 1×10 7 , 1×10 8 , 1×10 9 Five spore concentrations of 1 CFU / ml, 0.5% (w / w, accounting for the mass of the spore solution) Tween 80 was added dropwise and mixed for later use. Take sensitive strains of 1-3 instar nymphs, 3-5 instar nymphs, 5-7 instar female and male nymphs, male and female adult German cockroach healthy individuals, each group of 20, and use a micropipette to draw 1μL of spore suspension of different concentrations into The German cockroach dorsal plate began to die after 24 hours and was observed to 20 days. The cumulative mortality of the German cockroach was recorded. The experimental data was processed with SPSS20, and each group of experiments was repeated th...

Embodiment 3

[0059] The Metarhizium anisopliae of the present invention has strong selectivity and can be obtained through experiments. The fungus has outstanding pathogenic ability to Blattaria, especially Blattella germanica, and has relatively low pathogenic ability to other Blattaria. , The effect is not significant; there is no toxic damage to vertebrates (fish, etc.) and other common beneficial economic insects (such as spiders, parasitic wasps, etc.), which is beneficial to protect natural enemies and is safe for the environment.

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PUM

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Abstract

The invention discloses a pathogenic fungus for biological control of cockroaches. The classification name of the bacterial strain is metarhizium anisopliae which has been preserved in the China microbiological culture collection management committee general microbiological center with the serial number being EB0732 and the preservation number being CGMCC No.13170. The pathogenic fungus for biological control of cockroaches has the beneficial effects that (1) the pathogenic fungus is not prone to enabling insect pests to have resistance to chemicals; (2) the pathogenic fungus has strong selectivity for a host, and is safe for the environment; and (3) the pathogenic fungus has a strong lethal effect on male and female insect pests of different instars.

Description

Technical field [0001] The invention specifically relates to a pathogenic fungus for biological control of cockroaches. Background technique [0002] Cockroaches, commonly known as cockroaches, generally refer to insects belonging to the order Blattodea. According to literature, current taxonomists divide cockroaches into about 6000 species, of which about 50 are pests, such as the common Asian cockroach. In addition, the American cockroach (Periplaneta americana), German cockroach (Blatta germanica), brown cockroach and Australian cockroach are also widely distributed. There are more than 200 species in our country, and there are about 10 common indoor cockroaches, which vary from place to place. The common ones are Blattella germanica and Periplaneta americana. German cockroach (scientific name Blattella germanica), also known as German cockroach, German cockroach, originally from Africa, is a close relative of Asian cockroach. The German cockroach reproduces fast, and each o...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 张凡张宪翠张硕卢洁武婷王敏王誉蓉夏永明
Owner SHANDONG NORMAL UNIV
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