Metarhizium anisopliae IPPMHBC-009 and application thereof

A technology of scarab metarhizium anisopliae and locust, applied in the direction of application, fungi, biocides, etc., can solve the problems of fungal knockdown and slow action of killing pests, and achieve the effect of good application prospects

Active Publication Date: 2019-02-15
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of fungal knockdown and slow killing of pests in the prior art, the present invention provides a Metarhizium anisopliae IPPMHBC-009 and its application

Method used

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  • Metarhizium anisopliae IPPMHBC-009 and application thereof
  • Metarhizium anisopliae IPPMHBC-009 and application thereof
  • Metarhizium anisopliae IPPMHBC-009 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Screening of Metarhizium anisopliae IPPMHBC-009

[0032] Metarhizium anisopliae strains were lured from the collected soil by the method of trapping Mellonella mellonella. Put the sterilized waste film boxes into the soil samples to be tested, and bury one wax moth in each box, and repeat 3 times. The soil sample box is placed at 24-27°C, after moisturizing and trapping for 1 week, the dead insects are sorted out, cultured at a constant temperature of 25-26°C and relative humidity of 100 for 7-10 days, and the target strain is isolated from the dead insects, which is used for strain isolation The culture medium is PDAY.

[0033] Inoculate the isolated Metarhizium anisopliae on the PDAY solid medium, cultivate until spore production, suspend the spore powder with 0.1% Tween water, oscillate evenly with a vortex oscillator, and adjust the spore concentration to 1×10 8 individual / mL. Take 1 mL of the spore suspension and inoculate 100 mL of mycelia-producing m...

Embodiment 2

[0034] Example 2. Bioassay of the pathogenicity of Metarhizium anisopliae Cang 09 to the nymph Crypterus pubescens

[0035] The virulence effects of the above nine Metarhizium anisopliae strains on locusts were determined by stomach poisoning method.

[0036] First weigh 0.2g of Metarhizium anisopliae spore powder and add it to 2.0g of wheat bran, then add 0.1ml of edible oil, and stir well. Then weigh 0.2g of the prepared Metarhizium anisopliae wheat bran bait and put it into a petri dish, put it into a glass-covered insect rearing basket together with the petri dish, number them, and then put 20 of the same size into the insect rearing basket. 3rd instar hairy-legged locust gnat. No Metarhizium anisopliae spore powder was added to the wheat bran bait of the blank control group. In this experiment, there were 9 kinds of spore powders of Metarhizium anisopliae and one control CK. Each spore powder was set up for 3 repetitions and raised in the insect culture room. The bait ...

Embodiment 3

[0044] Embodiment 3. Metarhizium anisopliae is to the bioassay of the pathogenicity of Asian locust

[0045] Determination of 9 bacterial strains is to the curative power of Asian carcus, and assay method is the same as the method for embodiment 2.

[0046] The bioassay results of the pathogenicity of 9 strains of Metarhizium anisopliae to P. figure 2 ,Table 2.

[0047] Table 2 Comparison of the pathogenicity of 9 strains of Metarhizium anisopliae to P.

[0048]

[0049] From the analysis in Table 2, it can be seen that the tested strains are all pathogenic to locust gnats, the half-lethal time is between 3.55d and 10.67d, and the cadaveric rate is between 38.33% and 93.33%. The lethal time was 3.55 days, and the zombie rate was 93.33%. Therefore, there are significant differences between the Metarhizium anisopliae strain IPPMHBC-009 and the Metarhizium anisopliae strains IPPM803 and IPPM200614, indicating that the strain IPPMHBC-009 has higher pathogenicity to P.

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Abstract

The invention belongs to the technical field of biology, and particularly relates to Metarhizium anisopliae IPPMHBC-009 and an application thereof. The preservation number of the Metarhizium anisopliae IPPMHBC-009 is CGMCC No.16000, and the Metarhizium anisopliae IPPMHBC-009 has good median lethal time and cadaver rate for oedaleus decorus asiaticus and dasyhippus barbipes.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a metarhizium anisopliae IPPMHBC-009 and its application. Background technique [0002] Locusts are disastrous insects of Orthoptera Acrididae. Locust plagues occur frequently in my country, causing great damage to my country's agriculture. At present, the main means of controlling grassland locust damage in my country is to use chemical control methods, which have the advantages of fast, efficient, and flexible use. However, as people pay more and more attention to the sustainable development of the environmental economy, biological locust control methods have been developed. and apply it to actual work. Biological locust control methods, especially Metarhizium anisopliae, Beauveria bassiana, etc., have certain specificity, are harmless to humans and animals, and also have the advantages of no environmental pollution, no residue, and no resistance of pests. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 王广君郭隆隆李贝贝王晔青涂雄兵农向群张泽华
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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