Application of geraniol in inhibition of egg laying and egg incubation of tea lesser leafhopper

Abstract

The invention provides application of geraniol in inhibition of egg laying and egg incubation of tea lesser leafhopper, and belongs to the technical field of pest control. Empoasca flavescens oviposition and egg incubation are inhibited by fumigating geraniol. The influence of geraniol with different concentrations on the egg laying amount and egg hatching rate of the tea lesser leafhoppers is researched, and the result shows that egg laying and egg hatching of the tea lesser leafhoppers can be remarkably inhibited by adopting geraniol with the concentration of 1%, 5% and 10% for fumigation treatment. The geraniol can effectively reduce the population growth rate of the tea lesser leafhopper, is expected to be prepared into a natural preparation for preventing and treating the tea lesser leafhopper, and has the advantages of safety, high efficiency and sustainability.

Description

Technical Field

[0001] The present invention relates to the technical field of pest control, and particularly to the application of geraniol in inhibiting the oviposition and egg hatching of Empoasca vitis Background Art

[0002] Empoasca vitis is a kind of pest with the widest distribution and the most serious harm in tea plantations. Adults and nymphs of Empoasca vitis suck the sap of tender shoots and leaves of tea plants. The damaged buds and leaves turn yellow at the edges and red in the veins, and then the edges and tips of the leaves wither and scorch, the growth stagnates, the buds and leaves fall off, seriously affecting the yield and quality of tea leaves.

[0003] The large-scale and unreasonable use of chemical pesticides has led to the resistance of Empoasca vitis to many insecticides, increasing the difficulty of prevention and control, and seriously threatening the health of humans themselves and the sustainable development of the tea industry. Therefore, it is necessary to vigorously develop green prevention and control technologies that are safe, effective, and sustainable for controlling Empoasca vitis.

[0004] Plant essential oils are an important class of secondary metabolites existing in plants. They are mostly oily liquids at room temperature, volatile, and have strong fragrances and odors. Existing research shows that plant essential oils have effects such as attracting, repelling, antifeeding, fumigating, contact killing, and inhibiting the growth and development of pests.

[0005] Based on this, it is necessary to provide a plant essential oil that can effectively control Empoasca vitis. Summary of the Invention

[0006] The purpose of the present invention is to provide the application of geraniol in inhibiting the oviposition and egg hatching of Empoasca vitis, and to provide a new method for the prevention and control of Empoasca vitis.

[0007] In order to achieve the above-mentioned invention purpose, the present invention provides the following technical solutions:

[0008] The present invention provides the application of geraniol in inhibiting the oviposition and egg hatching of Empoasca vitis.

[0009] Preferably, the geraniol is used as the only active ingredient.

[0010] Preferably, the concentration of the geraniol is 1-10%.

[0011] Preferably, the oviposition and egg hatching of Empoasca vitis are inhibited by fumigating with geraniol.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] The present invention explores the effects of geraniol at different concentrations on the egg-laying amount and egg hatching rate of Empoasca vitis. The results show that fumigation treatment with geraniol at three concentrations of 1%, 5% and 10% can significantly inhibit the egg-laying and egg hatching of Empoasca vitis. This indicates that geraniol can effectively reduce the population growth rate of Empoasca vitis and is expected to be prepared into a natural preparation for controlling Empoasca vitis, with the advantages of safety, high efficiency and sustainability. Description of the Drawings

[0014] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained according to the provided drawings.

[0015] Figure 1 Calculation results of the number of eggs laid per female in different treatment groups in Example 1;

[0016] Figure 2 Calculation results of the egg hatching rate of Empoasca vitis in different treatment groups in Example 2. Detailed Embodiments

[0017] The following will describe in detail the technical solutions provided by the present invention in combination with the embodiments, but they cannot be understood as limiting the protection scope of the present invention.

[0018] The Empoasca vitis and tea branches used in the following embodiments were collected from Jianfengling National Forest Park, Ledong City, Hainan Province. The collected Empoasca vitis population was subcultured in a laboratory with environmental conditions of temperature 23.5±2°C, relative humidity 60±5%, and light-dark cycle 14h:10h (L:D).

[0019] Example 1 Inhibitory Effect of Geraniol on the Egg-Laying Amount of Empoasca vitis

[0020] Geraniol was mixed with water (containing 10% Tween 80) to prepare solutions with concentrations of 1%, 5% and 10% respectively and placed in centrifuge tubes.

[0021] Three-day-old and not yet sexually mature male and female Empoasca vitis (3 each) were introduced into a plastic jar (10×25 cm) for rearing. Three clean tea shoots without eggs were placed in the jar and cultivated with floral foam for water retention. At the same time, centrifuge tubes containing geraniol at different concentrations were placed in the plastic jar for fumigation treatment (the centrifuge tubes containing geraniol were replaced every 3 days), and water was used as a blank control (CK). Every 1 day, the tea shoots were taken out, and the number of cicada eggs on the tea shoots was observed and recorded with a blue light flashlight. At the same time, new tea shoots without eggs were replaced until no cicada eggs could be detected or all the adult Empoasca vitis in the plastic jar died.

[0022] The total number of cicada eggs was counted and the fecundity per female was calculated. The calculation results are as Figure 1 shown. It can be seen that compared with the CK group, the fumigation treatments with geraniol at three concentrations of 1%, 5% and 10% can significantly inhibit the fecundity per female of Empoasca vitis, indicating that geraniol has a good effect on inhibiting the oviposition of Empoasca vitis.

[0023] Example 2 Inhibitory effect of geraniol on the hatching rate of Empoasca vitis eggs

[0024] Geraniol was mixed with water (containing 10% Tween 80) to prepare solutions with concentrations of 1%, 5% and 10% respectively and placed in centrifuge tubes.

[0025] Sixty adult Empoasca vitis reared indoors were introduced into an insect rearing cage (25×25×35 cm), and 12 tea shoots without eggs were placed in the cage and cultivated with floral foam for water retention. The Empoasca vitis were allowed to lay eggs in the insect rearing cage. After 24 h, the tea shoots were taken out and detected with blue light. The tea shoots with egg grains were taken out separately and the number of cicada eggs was recorded. The tea shoots with egg grains were separately cultured in plastic jars containing centrifuge tubes with water (CK), 1%, 5% and 10% geraniol (the centrifuge tubes containing geraniol were replaced every 3 days). Three shoots were placed in each treatment and continued to be cultivated with floral foam for water retention. The number of successfully hatched eggs was recorded every day until the shoots withered completely.

[0026] After the experiment, the hatching rate of Empoasca vitis eggs was calculated. The results are as Figure 2 shown. It can be seen that the fumigation treatments with geraniol at three concentrations of 1%, 5% and 10% can significantly reduce the hatching rate of Empoasca vitis eggs.

[0027] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. Application of geraniol in inhibiting the oviposition and egg hatching of tea green leafhopper.

2. The use according to claim 1, characterized in that The geraniol is used as the sole active ingredient.

3. The use according to claim 1, characterized in that The concentration of the geraniol is 1-10%.

4. The use according to any one of claims 1 to 3, characterized in that: Inhibition of oviposition and egg hatching of tea green leafhopper by fumigation with geraniol.

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