Proliferation culture medium for tissue culture of Jietu vaccinium vitisidaea

A proliferation medium and tissue culture technology, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems affecting the rapid development and slow growth of lingonberry

Active Publication Date: 2014-06-25
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its fruit is blue or red, moderately sweet and sour, has high nutritional value and health care function, especially the antioxidant capacity is the first of all fruits and vegetables, it is beneficial to prevent human cell aging, prevent senile diseases (heart disease, cataract) , cancer, memory loss, etc.) have special effects, but

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Inoculate Jietu lingonberry into the improved WPM proliferation medium, and its medium components and contents include:

[0035] Macroelements: 1071.6 mg·L -1 Potassium nitrate, 370 mg·L -1 Magnesium sulfate, 96 mg·L -1 Calcium chloride, 170mg potassium dihydrogen phosphate;

[0036] Trace elements: 22.3 mg·L -1 Manganese sulfate, 8.6 mg·L -1 Zinc sulfate, 0.025 mg·L -1 Copper sulfate, 0.25mg sodium molybdate, 6.2 mg·L -1 boric acid;

[0037] Iron salt: 37.3 mg·L -1 Disodium edetate, 27.8 mg·L -1 ferrous sulfate;

[0038] Organic matter: 0.5 mg·L -1 Niacin, 0.5 mg·L -1 Thiamine hydrochloride, 0.5 mg·L -1 Pyridoxine hydrochloride, l00 mg·L -1 Inositol, 2 mg·L -1 Glycine;

[0039] Sucrose 30 g·L -1 , agar 8 g·L -1 ;

[0040] Zeatin 0.5 mg·L -1 ;

[0041] The rest is distilled water with a pH of 5.0;

[0042] The preparation method is as follows:

[0043] (1) Preparation of modified WPM medium:

[0044] (11) Preparation of 50-fold macroelement mothe...

Embodiment 2

[0058] Inoculate Jietu lingonberry into the improved WPM proliferation medium, and its medium components and contents include:

[0059] Macroelements: 1071.6 mg·L -1 Potassium nitrate, 370 mg·L -1 Magnesium sulfate, 96 mg·L -1 Calcium chloride, 170mg potassium dihydrogen phosphate;

[0060] Trace elements: 22.3 mg·L -1 Manganese sulfate, 8.6 mg·L -1 Zinc sulfate, 0.025 mg·L -1 Copper sulfate, 0.25mg sodium molybdate, 6.2 mg·L -1 boric acid;

[0061] Iron salt: 37.3 mg·L -1 Disodium edetate, 27.8 mg·L -1 ferrous sulfate;

[0062] Organic matter: 0.5 mg·L -1 Niacin, 0.5 mg·L -1 Thiamine hydrochloride, 0.5 mg·L -1 Pyridoxine hydrochloride, l00 mg·L -1 Inositol, 2 mg·L -1 Glycine;

[0063] Sucrose 30 g·L -1 , agar 9 g·L -1 ;

[0064] Zeatin 0.6 mg·L -1 ;

[0065] The rest is distilled water with a pH of 4.8;

[0066] The preparation method is as follows:

[0067] (1) Preparation of modified WPM medium:

[0068] (11) Preparation of 50-fold macroelement mothe...

Embodiment 3

[0081] The "Jietu" lingonberry was inoculated in the improved WPM proliferation medium, and its medium composition and content included:

[0082] Macroelements: 1071.6 mg·L -1 Potassium nitrate, 370 mg·L -1 Magnesium sulfate, 96 mg·L -1 Calcium chloride, 170mg potassium dihydrogen phosphate;

[0083] Trace elements: 22.3 mg·L -1 Manganese sulfate, 8.6 mg·L -1 Zinc sulfate, 0.025 mg·L -1 Copper sulfate, 0.25mg sodium molybdate, 6.2 mg·L -1 boric acid;

[0084] Iron salt: 37.3 mg·L -1 Disodium edetate, 27.8 mg·L -1 ferrous sulfate;

[0085] Organic matter: 0.5 mg·L -1 Niacin, 0.5 mg·L -1 Thiamine hydrochloride, 0.5 mg·L -1 Pyridoxine hydrochloride, l00 mg·L -1 Inositol, 2 mg·L -1 Glycine;

[0086] Sucrose 30 g·L -1 , agar 8 g·L -1 ;

[0087] Zeatin 0.8 mg·L -1 ;

[0088] The rest is distilled water with a pH of 4.9;

[0089] The preparation method is as follows:

[0090] (1) Preparation of modified WPM medium:

[0091] (11) Preparation of 50-fold macroele...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a proliferation culture medium for tissue culture of Jietu vaccinium vitisidaea. The proliferation culture medium comprises an improved WPM (woody plant medium), 2mg/L zeatin, 30g/L sucrose and 8g/L agar; the pH value is 4.8-5.0; each liter of improved WPM comprises a large quantity of elements including 1071.6mg of potassium nitrate, 370mg of magnesium sulfate, 96mg of calcium chloride and 170mg of potassium dihydrogen phosphate, ferric salts including 37.3mg of ethylene diamine tetraacetic acid and 27.8mg of ferrous sulfate, microelements including 22.3mg of manganese sulfate, 8.6mg of zinc sulfate, 0.025mg of copper sulfate, 0.25mg of sodium molybdate and 6.2mg of boric acid, and organic matters including 0.5mg of nicotinic acid, 0.5mg of thiamine hydrochloride, 0.5mg of pyridoxine hydrochloride, 100mg of inositol and 2mg of glycine. According to the culture medium, the proliferation rate reaches 9.8 after 30 days.

Description

technical field [0001] The invention relates to an improved lingonberry proliferation medium. Background technique [0002] Lingonberry is a small berry fruit tree belonging to the genus Vaccinium of the Rhododendron family (Ericaceae). Its fruit is blue or red, moderately sweet and sour, with high nutritional value and health care function, especially the antioxidant capacity is the first among all fruits and vegetables, which is helpful for preventing human cell aging and senile diseases (heart disease, cataract) , cancer, memory loss, etc.) have special effects, but conventional breeding methods cannot meet the needs of rapid development. There have been reports on the rapid propagation of bilberry by tissue culture. However, due to the slow growth rate of woody plant tissue culture, the rapid development of bilberry is still affected. It is extremely important to optimize the formula of bilberry medium. Contents of the invention [0003] In order to overcome the defi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 王姗王永平许建民王全智鲍华鹏
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap