Cell culture medium without animal component

Abstract

The invention provides an animal-source-free cell culture medium, which is prepared by proportionally mixing the components including calcium chloride, potassium chloride, anhydrous magnesium sulfate, sodium chloride, anhydrous sodium dihydrogen phosphate, L-arginine hydrochloride, L-cystine hydrochloride, L-glutamine, L-Leucine, L-isoleucine, L-histidine hydrochloride, L-tyrosine, D glucose, choline chloride, folic acid, nicotinamide, pyridoxal hydrochloride, riboflavin, thiamine hydrochloride and the like. Cells can be directly cultivated on the cell culture medium provided by the invention, rather than gradual domestication and cultivation, so that the density and the harvesting frequency of the cultured cells are increased and the expression quantity is increased so as to increase the vaccine yield in the field of medicine, thereby effectively avoiding the interference of animal-originated characteristics to the cultivated animal or human cells. The animal-source-free cell culture medium solves the problems that the cells cultivated by the prior serum-free medium have premature deaths and unsaturated forms.

Description

Technical field [0001] The present invention is an improvement on the existing cell culture medium components, and in particular provides an animal component-free cell culture medium, which belongs to the technical field of biological materials. Background technique [0002] Cell culture medium is the main biological material for culturing cells in the field of biotechnology, which directly affects the quality of cells. Only a good culture medium can screen and domesticate high-quality cells. The existing cell culture medium basically contains calf serum, which is used to maintain the growth and reproduction of cells in vitro for a long time. In view of the fact that serum contains some substances that are toxic to cells, it will affect cell growth and even cause cell death. It also increases the difficulty of separation and purification in industrial production. At present, serum-free medium has been available, but when the primary culture or cell growth is not good, it can only...

AI Technical Summary

Problems solved by technology

[0003] The invention provides a cell culture medium without animal components, which solves the problem that the existing serum-free medium cannot directly cultivate cells with poor growth conditions, and most cells need to gradually reduce the serum concentration to adapt to the existing serum-free medium and are susceptible to external conditions. factors that are likely to cause fatal damage

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