Cell culture medium without animal component

A culture medium and cell technology, applied in animal cells, tissue culture, microorganisms, etc., can solve the problems of susceptibility to external factors, lethal damage, etc., and achieve the effects of increasing density and harvest times, vigorous metabolism, and improving product purity
CN101381702AInactive Publication Date: 2009-03-11CHANGCHUN MEINENG BIOLOGICAL ENG
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
CHANGCHUN MEINENG BIOLOGICAL ENG
Publication Date
2009-03-11
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention provides an animal-source-free cell culture medium, which is prepared by proportionally mixing the components including calcium chloride, potassium chloride, anhydrous magnesium sulfate, sodium chloride, anhydrous sodium dihydrogen phosphate, L-arginine hydrochloride, L-cystine hydrochloride, L-glutamine, L-Leucine, L-isoleucine, L-histidine hydrochloride, L-tyrosine, D glucose, choline chloride, folic acid, nicotinamide, pyridoxal hydrochloride, riboflavin, thiamine hydrochloride and the like. Cells can be directly cultivated on the cell culture medium provided by the invention, rather than gradual domestication and cultivation, so that the density and the harvesting frequency of the cultured cells are increased and the expression quantity is increased so as to increase the vaccine yield in the field of medicine, thereby effectively avoiding the interference of animal-originated characteristics to the cultivated animal or human cells. The animal-source-free cell culture medium solves the problems that the cells cultivated by the prior serum-free medium have premature deaths and unsaturated forms.
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Description

Technical field

[0001] The present invention is an improvement on the existing cell culture medium components, and in particular provides an animal component-free cell culture medium, which belongs to the technical field of biological materials. Background technique

[0002] Cell culture medium is the main biological material for culturing cells in the field of biotechnology, which directly affects the quality of cells. Only a good culture medium can screen and domesticate high-quality cells. The existing cell culture medium basically contains calf serum, which is used to maintain the growth and reproduction of cells in vitro for a long time. In view of the fact that serum contains some substances that are toxic to cells, it will affect cell growth and even cause cell death. It also increases the difficulty of separation and purification in industrial production. At present, serum-free medium has been available, but when the primary culture or cell growth is not good, it can only...

Claims

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