Porcine pseudorabies virus strain as well as inactivated vaccine and applications thereof

A technology of porcine pseudorabies virus and porcine pseudorabies, applied in antiviral agents, viruses/bacteriophages, biochemical equipment and methods, etc., can solve the problems of poor immune induction effect, backward inactivation process, residual inactivation of inactivation agents, etc. problems, to achieve good immune protection efficacy and safety, good cell proliferation activity, and good immunogenicity
CN103305474AActive Publication Date: 2013-09-18泰州博莱得利生物科技有限公司 +2
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
泰州博莱得利生物科技有限公司
Publication Date
2013-09-18

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Abstract

The invention discloses a porcine pseudorabies virus strain as well as an inactivated vaccine and applications thereof, belonging to the field of separation and application of the porcine pseudorabies virus strain. The invention firstly provides a porcine pseudorabies virus BJ strain separated from diseased pig tissues, and the microbial preservation number of the porcine pseudorabies virus BJ strain is CGMCC (China General Microbiological Culture Collection Center) No.7351. The invention discloses a method for preparing the inactivated vaccine by applying the porcine pseudorabies virus BJ strain. The method comprises the steps of culturing a virus strain to obtain a virus solution; adding an inactivator, and inactivating and concentrating the virus solution; and evenly mixing an adjuvant and the virus solution, and emulsifying to obtain the inactivated vaccine. The technological parameters of the inactivated vaccine preparation method are further optimized, and the immune protection efficacy and safety of the inactivated vaccine can be improved. Shown by the immune protection efficacy and safety tests, the porcine pseudorabies inactivated vaccine prepared has good immune protection efficacy and safety, and can be clinically used for preventing or treating porcine pseudorabies.
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Description

technical field

[0001] The present invention relates to an isolated virus epidemic strain, in particular to an isolated porcine pseudorabies epidemic virus strain and an inactivated porcine pseudorabies vaccine prepared from the strain, belonging to the isolation and purification of porcine pseudorabies virus strains. application field. Background technique

[0002] Pseudorabies virus (Porcine pseudorabies virus, PRV) can cause a variety of diseases in domestic and wild animals with fever, itching (except pigs) and encephalomyelitis as the main symptoms. Immunization is the main strategy for preventing and controlling pseudorabies. Currently, the Bartha-K61 strain is widely used in China to prepare attenuated vaccines. Although the attenuated PRV gene deletion vaccine can effectively prevent the emergence of clinical symptoms after infection, the vaccinated pigs may still be infected by the strong virus, and the infection can form a latent virus, and then the latent infecte...

Claims

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