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Method for hybrid broussonetia papyrifera tissue culture

A tissue culture and tree-building technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of highly unfavorable seedling hardening, slow growth of tree-shaped trees, and poor growth, and achieve low production costs and tissue culture The effect of strong seedlings and rapid proliferation

Inactive Publication Date: 2016-05-04
靳杏子
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Problems solved by technology

[0003] The object of the present invention is to provide a method for tissue culture of hybrid tree, aiming at solving the problems of slow growth, poor growth and height that are not conducive to hardening seedlings in the existing method for tissue culture of hybrid tree

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  • Method for hybrid broussonetia papyrifera tissue culture

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Embodiment Construction

[0031] The present invention will be further described below in conjunction with accompanying drawing:

[0032] see figure 1 :

[0033] A method of hybrid tree tissue culture, comprising:

[0034] S101, collection of hybrid tree tissue culture explants, collection of twigs with axillary buds;

[0035] S102, cleaning and disinfecting the explants collected outdoors;

[0036] S103. Induction of explant callus after disinfection treatment;

[0037] S104, callus redifferentiation and subculture;

[0038] S105, rooting culture of virus-free seedlings;

[0039] S106, seedling hardening;

[0040] S107, field planting.

[0041] Further, the length of the collected explants is between 2-4 cm.

[0042] Further, the specific methods for cleaning and disinfecting the explants collected outdoors are as follows:

[0043] Rinse with running water for 5 minutes, soak in 1% detergent water for 5 minutes, disinfect with 70% ethanol, disinfect for 30 seconds, rinse with sterile water 4-...

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Abstract

The invention discloses a method for hybrid broussonetia papyrifera tissue culture. The method includes the steps that a hybrid broussonetia papyrifera tissue culture explant is acquired, and a twig with an axillary bud is acquired; the explant acquired outdoors is cleaned and disinfected; calluses of the disinfected explant are induced; the calluses are re-differentiated and sub-cultured; a virus-free seedling is subjected to rooting culture; the seedling is hardened; field planting is conducted. The method for hybrid broussonetia papyrifera tissue culture can be effectively improved, the tissue culture time of hybrid broussonetia papyrifera can be effectively shortened, the quality of hybrid broussonetia papyrifera tissue culture seedlings can be improved, multiplication is rapid, the tissue culture seedlings are strong, rooting is rapid, production cost is low, contamination is low, and time spent from explant inoculation to seedling emerging is short.

Description

technical field [0001] The invention belongs to the field of tree planting, and in particular relates to a method for tissue culture of a hybrid tree. Background technique [0002] At present, the methods of hybrid tree tissue culture are different, but all are not very ideal. The growth of the tree is slow, the growth is not good, and the height is not conducive to seedling hardening. Contents of the invention [0003] The purpose of the present invention is to provide a method for tissue culture of hybrid tree mulberry, aiming at solving the problems of slow growth, poor growth and height unfavorable for hardening seedlings in the existing method for tissue culture of hybrid tree mulberry. [0004] The present invention is achieved in that a kind of method of hybrid tree tissue culture comprises: [0005] Step 1, collection of hybrid tree tissue culture explants, collection of twigs with axillary buds; [0006] Step 2, cleaning and disinfecting the explants collected o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 靳杏子杨进何平礼王菊玲李鑫靳木子
Owner 靳杏子
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