A method for tissue culture and rapid propagation of Ilex red fruit superior strain

Abstract

The invention discloses a tissue culture and rapid propagation method for an ilex purpurea superior plant and belongs to the technical field of plant tissue culture. The method comprises the followingsteps: obtaining an explant; selecting an annual tender shoot tip of the ilex purpurea superior plant, and cleaning, disinfecting and sterilizing the same; induced culture: transferring the explant into an induced culture medium to induce new buds; enrichment culture: transferring the new buds to an enrichment medium to be cultured for 30 days to obtain the proliferated shoot buds; rooting culture: slicing the shoot tips of the shoot buds and transferring the shoot tips into a rooting culture medium to be cultured for 15 days to obtain rooting seedlings; and acclimatization and transplant: sealing the rooting seedlings for 2-3 days and acclimatizing the rooting seedlings with a film for 10 days and then transplanting the rooting seedlings. The tissue culture and rapid propagation method disclosed by the invention improves the propagation coefficient of ilex purpurea, shortens the rooting culture period greatly and improves the propagation speed. Compared with a conventional ilex purpurea tissue culture method, the method provided by the invention has higher survival rate and higher adaptability.

Description

Technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture and rapid propagation method for superior plants of Ilex rubra. technical background [0002] Ilex purpurea Hassk, an evergreen tree of Ilex in Aquifoliaceae. Red holly has both medicinal value and ornamental value. Chinese Pharmacopoeia clearly records that red holly Hollyleaf can be used as medicine, which has great medicinal value, and is also the main component of commonly used Chinese patent medicine Jizhi syrup formula. Ilex rubra is dioecious, and the female plant is full of red fruits in autumn, which is bright and beautiful, and has a long fruiting period, which can last from October of that year to April to May of the following year. It is a landscape tree species with high ornamental value. [0003] Ilex pedunculata is a chemical substance with great value in Ilex rubra, which has the functions of clearing away heat and toxic materials, reducin...

AI Technical Summary

Problems solved by technology

At present, the medicinal materials of holly holly mainly come from wild resources, and the content of medicinal components of each plant varies greatly, which cannot be supplied stably, which is not enough to meet the needs of the pharmaceutical industry.

At present, Ilex is mainly propagated by seeds and cuttings. The propagation efficiency of the above two methods is low and cannot meet the production requirements for rapid propagation of fine varieties; occasionally, it is propagated by tissue culture

However, the tissue culture propagation method of the prior art may have high pollution rate, difficult rooting, slow growth, low survival rate of tissue culture seedlings, etc. when breeding and regenerating plants, which is unfavorable for the cultivation of holly holly tissue culture seedlings.

[0004] The patent application number is 201410478843.2 for the invention patent "a method of tissue culture and propagation of golden leaf tortoise shell holly", through disinfection and sterilization, primary culture, subculture, strong seedling culture, rooting culture, seedling hardening, transplanting steps, etc. to propagate golden leaf Tortoiseshell holly can improve the propagation coefficient of golden leaf tortoiseshell holly, accelerate the propagation speed of golden leaf tortoiseshell holly, improve the survival rate of golden leaf tortoiseshell holly, but the propagation method of golden leaf tortoiseshell holly can not be used for the propagation of red fruit holly, and There are many reproduction steps and the cycle is too long, which is not conducive to the rapid reproduction of Ilex spp., and there is no mention of the problem of reducing the pollution rate during the tissue culture process