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Method for building leaf in vitro regeneration system of begonia rex

A technology of in vitro regeneration and begonia, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of low overall proliferation rate and long tissue culture cycle.

Inactive Publication Date: 2013-08-07
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The research group of this application once published an article "Research on Tissue Culture Technology of Toad Leaf Begonia "Bright Can"", Journal of Jiangxi Agricultural Sciences, No. 12, 2011. However, its overall proliferation rate is low, and the proliferation rate in one culture cycle (about 70d) is only 80.5 times, and the tissue culture cycle is relatively long, in the prior art, there is an urgent need for a high-efficiency in vitro rapid propagation method of begonia

Method used

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  • Method for building leaf in vitro regeneration system of begonia rex
  • Method for building leaf in vitro regeneration system of begonia rex
  • Method for building leaf in vitro regeneration system of begonia rex

Examples

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Embodiment Construction

[0023] Explant Screening Example:

[0024] 1. Screening of the placement mode of explants in the culture medium

[0025] For the leaves of explants with similar size, shape and growth state, the different placement methods also lead to different culture effects. The explants placed on the abaxial surface germinated early, had a large number, and grew extremely fast, while the leaves placed on the adaxial surface had almost no bud point differentiation, and they gradually tended to brown or wilt after being placed for more than 30 days.

[0026] Table 1

[0027]

[0028] Media Screening Example

[0029] 2. Screening of Adventitious Bud Induction Medium

[0030] Cut the sterilized into 0.5-1.0cm 2 The leaf pieces were inserted into 5 kinds of media for adventitious bud induction culture for 10-20d, the culture temperature was 25±2°C, the light time was 14h / d, and the light intensity was 1300lx. It was found that the medium with the best effect of inducing differentiation...

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Abstract

The invention discloses a method for building a leaf in vitro regeneration system of begonia rex. The method comprises the following steps of: (1) selecting an explant and sterilizing the explant; (2) carrying out adventitious bud induced cultivation after cutting the processed leaves; (3) carrying out high-voltage electrostatic field treatment; (4) carrying out subculture proliferation cultivation on the adventitious bud induced by the leaves; (5) carrying out rooting culturing on a test-tube plantlet after the subculture proliferation cultivation; and (6) cutting and rooting. The begonia rex is subjected to tissue culturing by adopting the propagation method; the reproduction rate of a culturing period (51d) is 243.1 times; the propagation speed is fast; and the problem of seeding propagation of the begonia rex is effectively solved.

Description

technical field [0001] The invention specifically relates to a propagation method of toad leaf begonia (Begonia rex) tissue culture. Background technique [0002] Toad leaf begonia (Begonia rex), a perennial herbaceous foliage plant of the genus Begonia in the family Begoniaceae, is native to Brazil and the eastern part of the bream. It has beautiful leaf shape and gorgeous leaf color, and is an excellent indoor foliage plant. It likes warm, humid, semi-shady and humid environments, avoiding direct sunlight. The suitable temperature for growth is 22-25°C, and it is not resistant to high temperature. If the temperature exceeds 32°C, the growth will be slow. [0003] Toad leaf begonia, the rootstock is thick and short, the leaves are broad and ovate, and there are deep wavy teeth on the edge. The leaves are green, with dark green stripes on the leaf surface, silvery white stripes in the middle, and purplish red on the back of the leaves. The leaves and petioles are densely ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 宣继萍郭忠仁徐菲刘永芝贾晓东
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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