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A kind of rapid propagation method of Trichosanthes chinensis

A high-speed technology, which is applied to the field of rapid propagation of Gaotang, can solve the problems of indistinguishable, insignificant effect, long required period, etc., and achieves a simple treatment method, shortens the tissue culture time, and maintains excellent quality. Effects of Features

Active Publication Date: 2018-09-04
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the dioecious plants of Trichosanthes, there is no obvious morphological difference before flowering, so it is difficult to distinguish. After planting, it often leads to many male plants (the ratio of male and female plants is about 3:7), which affects the yield and economic benefits. Using split roots to reproduce, because The root itself is also a medicinal material, and the reproduction of the root is not large, and the provenance is seriously degraded due to perennial asexual reproduction, resulting in a small number of high-quality seedlings
[0003] Therefore, in order to solve the rapid reproduction of Gaotang Trichosanthes, there are many breeding methods, but the effect is not obvious, and the survival rate is also very problematic.
Most of the existing reports carry out rapid propagation of Trichosanthes chinensis through callus induction, which has problems such as high mutation rate and long period required

Method used

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  • A kind of rapid propagation method of Trichosanthes chinensis

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Experimental program
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Effect test

Embodiment 1

[0021] A kind of rapid propagation method of Trichosanthes grandiflora, comprising the following steps:

[0022] 1) Selection and treatment of explants: Take healthy tuber seedlings of the male and female plants of Trichomona gaotang, strip off the terminal buds, rinse with running water for 30 minutes, place them on a clean bench, and soak the explants in 70% ethanol 1 minute, rinse twice with sterile water, disinfect with 0.1% mercury liter for 5 minutes, rinse with sterile water 5-6 times, dry the water with sterile filter paper, and set aside;

[0023] 2) Induction of clustered buds: Inoculate the explants treated in step 1) on the induction medium to obtain clustered shoots, divide the clustered shoots into individual plants and culture them continuously for 2 generations on the induction medium to obtain regenerated plants

[0024] 3) Root induction: Transplant the regenerated plants into the rooting medium, induce rooting to obtain plants with developed root systems;

...

Embodiment 2

[0032] A kind of rapid propagation method of Trichosanthes grandiflora, comprising the following steps:

[0033] 1) Selection and treatment of explants: take healthy tuber seedlings of the female and male plants of Trichomona gaotang, strip off the terminal buds, rinse with running water for 30 minutes, place them on an ultra-clean workbench, and soak the explants in 70% ethanol 1 minute, rinse twice with sterile water, disinfect with 0.1% mercury liter for 5 minutes, rinse with sterile water 5-6 times, dry the water with sterile filter paper, and set aside;

[0034] 2) Induction of clustered buds: Inoculate the explants treated in step 1) on the induction medium to obtain clustered shoots, divide the clustered shoots into individual plants and culture them continuously for 2 generations on the differentiation medium to obtain regenerated plants

[0035] 3) Root induction: Transplant the regenerated plants into the rooting medium, induce rooting to obtain plants with developed...

Embodiment 3

[0042] Embodiment 3 A kind of rapid propagation method of Trichosanthes chinensis, comprising the following steps:

[0043] 1) Selection and treatment of explants: take healthy tuber seedlings of the female and male plants of Trichomona gaotang, strip off the terminal buds, rinse with running water for 30 minutes, place them on an ultra-clean workbench, and soak the explants in 70% ethanol 1 minute, rinse twice with sterile water, disinfect with 0.1% mercury liter for 5 minutes, rinse with sterile water 5-6 times, dry the water with sterile filter paper, and set aside;

[0044] 2) Induction of clustered buds: Inoculate the explants treated in step 1) on the induction medium to obtain clustered shoots, divide the clustered shoots into individual plants and culture them continuously for 2 generations on the induction medium to obtain regenerated plants;

[0045] 3) Root induction: Transplant the regenerated plants into the rooting medium, induce rooting to obtain plants with dev...

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Abstract

The invention provides a rapid Gaotang trichosanthes kirilowii propagation method which comprises the following steps: 1) selecting and treating an explant; 2) performing clustering bud induction; 3) performing root induction; 4) transplanting. The vegetative propagation speed of Gaotang trichosanthes kirilowii can be increased, and excellent characteristics of a female parent can be maintained. The method is high in bud ratio, short in culture time, high in operability, high in application value, low in investment and high in yield.

Description

technical field [0001] The invention belongs to the field of plant planting, and in particular relates to a method for rapid propagation of Trichosanthes grandis. Background technique [0002] Gaotang Trichosanthes is a kind of authentic medicinal material in Shandong. It is large and high-quality, and enjoys a high reputation at home and abroad. In recent years, the area has continued to expand, but the supply of high-quality seedlings is insufficient. Trichosanthes propagation methods are usually seed propagation and root division propagation. Due to the dioecious plants of Trichosanthes, there is no obvious morphological difference before flowering, so it is difficult to distinguish. After planting, it often leads to many male plants (the ratio of male and female plants is about 3:7), which affects the yield and economic benefits. Using split roots to reproduce, because The tuber root itself is also a medicinal material, and at the same time, the root tuber reproduction ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 彭向前李光勇刘辉张静静安南
Owner LIAOCHENG UNIV
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