Method for propagating betula luminifera aseptic seedlings by using tissue culture technology

A technology of tissue culture and sterile seedlings, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve problems such as tight supply and demand, long growth cycle of forest trees, etc., to shorten germination time, improve emergence efficiency, and increase the number of seeds Effect

Pending Publication Date: 2021-01-08
毕节市林业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nowadays, with the rapid development of my country's economy, the demand for wood has also increased significantly, but the long growth cycle of forest trees has caused a serious problem of tight supply and demand.

Method used

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  • Method for propagating betula luminifera aseptic seedlings by using tissue culture technology
  • Method for propagating betula luminifera aseptic seedlings by using tissue culture technology
  • Method for propagating betula luminifera aseptic seedlings by using tissue culture technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A kind of method utilizing tissue culture technology to propagate the aseptic seedling of Betula glabra, comprising the following steps:

[0043] 1. Collection of explants: Collect mature seeds on sunny days, and cut the tender stems of Birch glabra with axillary buds growing well.

[0044] 2. Disinfection of explants:

[0045] a. Cut the collected tender stems into 4cm stem segments, peel off the seed coats of the seeds, place them in a clean beaker, soak them in detergent water for 30 minutes, and then rinse them with running water;

[0046] b. The washed stems and stems are treated with PEF with an electric field strength of 10Kv / cm, a pulse width of 35μs, and a pulse frequency of 700Hz, wherein the stems are treated for 15s and the seeds are treated for 22s;

[0047] c. Place the treated stems and seeds on an ultra-clean workbench, first soak them with 75% alcohol for 45 seconds, rinse them with sterile water for 5 times, then soak them with 0.1% mercury liter for ...

Embodiment 2

[0069] Under the same conditions as in Example 1, each culture medium was replaced with:

[0070] (1) Adventitious bud induction medium: its composition is: MS+6-BA 2.0mg / L+2,4-D 0.05mg / L+NAA0.4mg / L+agar 6.5g / L+sucrose 25g / L; pH condition It is 5.8; it is prepared by sterilizing at 121℃101KPa for 20min.

[0071] (2) Proliferation medium: its composition is: MS+2,4-D 3.0mg / L+NAA 2.0mg / L+IAA 1.0mg / L+agar 6.5g / L+sucrose 30g / L; pH condition is 6.0; Sterilized at 121°C and 101KPa for 20 minutes.

[0072] (3) Seed germination induction medium, which consists of: MS+6-BA 3.0mg / L+NAA 0.25mg / L+GA3 2.0mg / L+6.5g / L carrageenan+25g / L sucrose; the pH condition is 6.0; prepared by sterilizing at 121°C and 101KPa for 20 minutes.

[0073] (4) Callus induction medium: its composition is: MS+6-BA 2.0mg / L+NAA 0.5mg / L+TDZ 1.0mg / L+6.5g / L carrageenan+25g / L sucrose; pH condition It is 5.9; it is prepared by sterilizing at 121℃101KPa for 20min.

[0074] (5) Cluster bud induction medium: its compo...

Embodiment 3

[0084] Under the same conditions as in Example 1, each culture medium was replaced with:

[0085] (1) Adventitious bud induction medium: its composition is: MS+6-BA 1.5mg / L+2,4-D 0.1mg / L+NAA 0.3mg / L+agar 6.5g / L+sucrose 30g / L; pH conditions It is 5.8; it is prepared by sterilizing at 121℃101KPa for 20min.

[0086] (2) Proliferation medium: its composition is: MS+2,4-D 4.0mg / L+NAA 1.5mg / L+IAA 2.0mg / L+agar 6.5g / L+sucrose 25g / L; the pH condition is 6.0; Sterilized at 121°C and 101KPa for 20 minutes.

[0087] (3) Seed germination induction medium, which consists of: MS+6-BA 4.0mg / L+NAA 0.4mg / L+GA3 3.0mg / L+6.5g / L carrageenan+25g / L sucrose; the pH condition is 6.0; prepared by sterilizing at 121°C and 101KPa for 20 minutes.

[0088] (4) Callus induction medium: its composition is: MS+6-BA 1.5mg / L+NAA 0.3mg / L+TDZ0.75mg / L+6.5g / L carrageenan+30g / L sucrose; pH condition It is 5.9; it is prepared by sterilizing at 121℃101KPa for 20min.

[0089] (5) Cluster bud induction medium: its c...

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Abstract

The invention belongs to the technical field of vegetative propagation of plants, and particularly relates to a method for propagating betula luminifera aseptic seedlings by using a tissue culture technology. According to the method, betula luminifera aseptic seedlings are propagated through the tissue culture technology, improvement is conducted on the basis of a traditional method, explants aredisinfected, differentiation promotion treatment is conducted in the culture process, the culture time is shortened on the basis of an existing tissue culture technology, and a large number of virus-free betula luminifera aseptic seedlings consistent in character are obtained in a short time; and tender stems and seeds can also be separately adopted for culture, betula luminifera is fully utilized, the characteristics of a female parent are kept to the maximum extent, and scientific and technological support is provided for betula luminifera resource protection, improved variety breeding and industrial development.

Description

technical field [0001] The invention belongs to the technical field of plant asexual reproduction, and in particular relates to a method for propagating aseptic seedlings of Betula glabra by tissue culture technology. Background technique [0002] Betula luminifera, also known as bright-leaved birch, bright bark tree, and birch horn, is a tall deciduous tree of the family Betulaceae, and is a unique and excellent fast-growing wood tree species in my country. Birch is widely distributed, grows fast, has strong adaptability, excellent material and wide application. , fast-growing, and broad-leaved tree species, not only have a short growth cycle and excellent materials, which can fully meet the needs of the Chinese market, but also solve a series of problems such as single species of afforestation and excessive proportion of fir and pine, and have broad application prospects. Since the 1980s, the demand for birch lumber in the international market has continued to rise. Nowa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李斌侯俊毕宁王彩云肖艳周聿
Owner 毕节市林业科学研究所
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