Tissue culture method of ginseng adventitious root

A technique of tissue culture and ginseng, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of high yield, high induction rate, and simple process

Inactive Publication Date: 2009-12-23
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many reports on ginseng tissue culture in my country, but they mainly focus on the

Method used

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  • Tissue culture method of ginseng adventitious root
  • Tissue culture method of ginseng adventitious root
  • Tissue culture method of ginseng adventitious root

Examples

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Comparison scheme
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Example Embodiment

[0010] Example 1:

[0011] Adjust 100 mL of MS solid medium containing 1 mg / L 2,4-D, 0.1 mg / L KT, and 30 g / L sucrose to pH 5.8 with 1 mol / L HCl and NaOH solution. Bacteria for 15 minutes; the fresh roots of Northeast Jilin cultivated ginseng (provided by the Zuojia Specialty Research Institute of the Chinese Academy of Agricultural Sciences) are rinsed with tap water, dried in an oven at 45℃, and then sprayed with 75% ethanol in an ultra-clean table Burn the surface with an alcohol lamp flame to dryness for disinfection and sterilization, then use a sterilized scalpel to make a 1cm incision on the root surface, and cut it from both sides, and then cut the internal tissue into small pieces of about 0.5cm. The blocks were received in the pre-prepared MS solid medium and cultured under sterile, 23°C and dark conditions to induce callus. After 4 weeks of culture, the formation of callus could be seen. Adjust 100 mL of MS solid medium containing 1 mg / L 2,4-D, 0.1 mg / L KT, and 30 g / L su...

Example Embodiment

[0012] Example 2:

[0013] Adjust 100 mL of MS solid medium containing 2 mg / L 2,4-D, 0.5 mg / L KT, and 30 g / L sucrose to pH 5.9 with 1 mol / L HCl and NaOH solution, and quench at 121°C and 0.1 MPa pressure. Bacteria for 20 minutes; rinse the fresh roots of Northeast Jilin cultivated ginseng (provided by Zuojia Specialty Research Institute of Chinese Academy of Agricultural Sciences) with tap water, dry in an oven at 50°C, and spray with 75% ethanol in an ultra-clean table Burn the surface with an alcohol lamp flame to dryness for disinfection and sterilization, then use a sterilized scalpel to make a 2cm incision on the root surface, and cut it from both sides, and then cut the internal tissue into small pieces of about 1cm After receiving the MS solid medium prepared in advance, culture it under sterile, 24°C and dark conditions to induce callus. After 3 weeks of culture, the formation of callus can be seen. Adjust 100 mL of MS solid medium containing 2 mg / L 2,4-D, 0.5 mg / L KT, and...

Example Embodiment

[0014] Example 3:

[0015] Adjust 100mL of MS solid medium containing 4mg / L 2,4-D, 1mg / L KT, 30g / L sucrose to pH 6.0 with 1mol / L HCl and NaOH solution, and sterilize at 121℃ and 0.1MPa pressure 25 minutes standby; rinse the fresh roots of Northeast Jilin cultivated ginseng (provided by the Zuojia Specialty Research Institute of Chinese Academy of Agricultural Sciences) with tap water, dry in an oven at 50℃, and then spray the surface with 75% ethanol in an ultra-clean table , Burn it with an alcohol lamp to dryness for disinfection and sterilization, then use a sterilized scalpel to make a 2cm incision on the root surface, and cut it from both sides, and then cut the internal tissue into small pieces of about 0.5cm After receiving the MS solid medium prepared in advance, culture it under sterile, 25°C and dark conditions to induce callus. After 3 weeks of culture, the formation of callus can be seen. Adjust 100mL of MS solid medium containing 4mg / L 2,4-D, 1mg / L KT, 30g / L sucrose t...

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Abstract

The invention discloses a tissue culture method of ginseng adventitious root, comprising the steps of induction, amplification and successive transfer culture of ginseng callus; the induced callus is inoculated on the prepared solid culture medium and added with plant growth regulator to be cultured for 3-4 weeks under the conditions of sterility, the temperature of 23-25 DEG C and keeping in dark place; the amplified callus is inoculated on the prepared solid culture medium and added with proper plant growth regulator to be cultured for 3-5 weeks under the conditions of sterility, the temperature of 23-25 DEG C and keeping in dark place, and then the adventitious root can be formed; the formed adventitious root is picked out and inoculated on the prepared liquid culture medium and added with the plant growth regulator to be cultured for 4-5 weeks under the conditions of sterility, room temperature and sunshine. The method has higher actual significance than ginseng cell cultivation, and the growth speed of the adventitious root is much higher than that of the original root; furthermore, the production rate of active secondary metabolite of the adventitious root is high and stabler and is not influenced by natural environment such as natural disaster and the like; the tissue culture method also has the characteristics of simple technique process, high inductivity, good repeatability, rapid propagation of the adventitious root, etc.

Description

Technical field: [0001] The invention discloses a ginseng adventitious root tissue culture method, which belongs to the field of traditional Chinese medicine ginseng tissue culture. Background technique: [0002] Ginseng (Panax ginseng) is a perennial dicotyledonous plant of the genus Panax in the family Araliaceae, and its roots are most commonly used as medicine. Modern pharmacological studies have shown that ginseng has the functions of regulating glucose metabolism, enhancing immunity, delaying aging, anti-tumor, lowering blood pressure, preventing and treating cardiovascular diseases, protecting liver and liver, enhancing learning ability and memory ability, and intelligence development. Due to years of over-harvesting, the forest ecological environment on which ginseng depends has been severely damaged, and wild ginseng resources have been exhausted. Currently, cultivated ginseng is mostly used for medicinal purposes. However, the cultivation and production cycle of g...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 高文远黄滔王娟
Owner TIANJIN UNIV
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