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Tissue culture method of ginseng adventitious root

A technique of tissue culture and ginseng, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of high yield, high induction rate, and simple process

Inactive Publication Date: 2009-12-23
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many reports on ginseng tissue culture in my country, but they mainly focus on the culture of callus and suspension cells, and the culture of adventitious roots has not been reported yet.

Method used

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  • Tissue culture method of ginseng adventitious root
  • Tissue culture method of ginseng adventitious root
  • Tissue culture method of ginseng adventitious root

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0011] Adjust 100 mL of MS solid medium containing 1 mg / L 2,4-D, 0.1 mg / L KT, and 30 g / L sucrose to pH 5.8 with 1 mol / L HCl and NaOH solution, and quench at 121 °C under a pressure of 0.1 MPa. Bacteria 15 minutes standby; The fresh root (provided by Zuojia Institute of Special Products, Chinese Academy of Agricultural Sciences) of Northeast Jilin cultivated ginseng is rinsed with tap water, as for drying in 45 ℃ oven, spray with 75% ethanol then in ultra-clean bench On the surface, burn it with an alcohol lamp flame until it is dry for disinfection and sterilization, then use a sterilized scalpel to cut a 1cm incision on the root surface, and cut it off from both sides, and then cut the internal tissue into small pieces of about 0.5cm The blocks were connected to the pre-prepared MS solid medium, cultured under sterile, 23°C and light-proof conditions to induce callus, and the formation of callus could be seen after 4 weeks of culture. Adjust 100 mL of MS solid medium containi...

example 2

[0013] Adjust 100 mL of MS solid medium containing 2 mg / L 2,4-D, 0.5 mg / L KT, and 30 g / L sucrose to pH 5.9 with 1 mol / L HCl and NaOH solution, and quench at 121 °C under a pressure of 0.1 MPa. Bacteria 20 minutes standby; The fresh root (provided by Zuojia Institute of Specialty Products, Chinese Academy of Agricultural Sciences) of Northeast Jilin cultivated ginseng is rinsed with tap water, dried in a 50°C oven, then sprayed with 75% ethanol in an ultra-clean bench On the surface, burn it with an alcohol lamp flame until it is dry for disinfection and sterilization, then use a sterilized scalpel to cut a 2cm incision on the root surface, and cut it off from both sides, and then cut the internal tissue into small pieces of about 1cm Received in MS solid medium prepared in advance, cultured under sterile, 24°C and light-proof conditions to induce callus, and the formation of callus was visible after 3 weeks of culture. Adjust 100 mL of MS solid medium containing 2 mg / L 2,4-D, ...

example 3

[0015] Adjust 100 mL of MS solid medium containing 4 mg / L 2,4-D, 1 mg / L KT, and 30 g / L sucrose to pH 6.0 with 1 mol / L HCl and NaOH solution, and sterilize at 121 °C under a pressure of 0.1 MPa Standby in 25 minutes; the fresh root of ginseng cultivated in Jilin, Northeast China (provided by Zuojia Institute of Special Products, Chinese Academy of Agricultural Sciences) is rinsed with tap water, dried in a 50°C oven, and sprayed with 75% ethanol on the surface in an ultra-clean bench , burned to dry with an alcohol lamp flame to sterilize, then cut a 2cm incision on the root surface with a sterilized scalpel, and cut off from both sides, and then cut the internal tissue into small pieces of about 0.5cm Received in MS solid medium prepared in advance, cultured under sterile, 25°C and light-proof conditions to induce callus, and the formation of callus was visible after 3 weeks of culture. Adjust 100 mL of MS solid medium containing 4 mg / L 2,4-D, 1 mg / L KT, and 30 g / L sucrose to ...

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Abstract

The invention discloses a tissue culture method of ginseng adventitious root, comprising the steps of induction, amplification and successive transfer culture of ginseng callus; the induced callus is inoculated on the prepared solid culture medium and added with plant growth regulator to be cultured for 3-4 weeks under the conditions of sterility, the temperature of 23-25 DEG C and keeping in dark place; the amplified callus is inoculated on the prepared solid culture medium and added with proper plant growth regulator to be cultured for 3-5 weeks under the conditions of sterility, the temperature of 23-25 DEG C and keeping in dark place, and then the adventitious root can be formed; the formed adventitious root is picked out and inoculated on the prepared liquid culture medium and added with the plant growth regulator to be cultured for 4-5 weeks under the conditions of sterility, room temperature and sunshine. The method has higher actual significance than ginseng cell cultivation, and the growth speed of the adventitious root is much higher than that of the original root; furthermore, the production rate of active secondary metabolite of the adventitious root is high and stabler and is not influenced by natural environment such as natural disaster and the like; the tissue culture method also has the characteristics of simple technique process, high inductivity, good repeatability, rapid propagation of the adventitious root, etc.

Description

Technical field: [0001] The invention discloses a ginseng adventitious root tissue culture method, which belongs to the field of traditional Chinese medicine ginseng tissue culture. Background technique: [0002] Ginseng (Panax ginseng) is a perennial dicotyledonous plant of the genus Panax in the family Araliaceae, and its roots are most commonly used as medicine. Modern pharmacological studies have shown that ginseng has the functions of regulating glucose metabolism, enhancing immunity, delaying aging, anti-tumor, lowering blood pressure, preventing and treating cardiovascular diseases, protecting liver and liver, enhancing learning ability and memory ability, and intelligence development. Due to years of over-harvesting, the forest ecological environment on which ginseng depends has been severely damaged, and wild ginseng resources have been exhausted. Currently, cultivated ginseng is mostly used for medicinal purposes. However, the cultivation and production cycle of g...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 高文远黄滔王娟
Owner TIANJIN UNIV
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