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Common chlorella as well as culturing method and application thereof

The technology of a common chlorella and its cultivation method is applied in the field of common chlorella and its cultivation, which can solve the problems of hindered industrialization, high production cost, and poor tolerance, and achieve unique and ingenious design, high production intensity, high The effect of comprehensive economic and social benefits

Active Publication Date: 2012-07-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing microalgae used as raw materials for bioenergy are mainly CO 2 Poor tolerance, low production intensity, and high-concentration medium for cultivation, high production costs, and hindered industrialization

Method used

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  • Common chlorella as well as culturing method and application thereof
  • Common chlorella as well as culturing method and application thereof
  • Common chlorella as well as culturing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Using the fermentation method of segmentation method-two-step method, and using the secondary effluent of municipal sewage and industrial sewage treated by sewage treatment plants after filtering out bacteria or algae as the medium, inoculate the medium with Chlorella C9-JN2010, where the initial density is 6.0×10 7 cfu / ml, the light cycle is 16h:8h, the light intensity is 15000lux, the culture temperature is 30℃, the pH is 7.0, CO 2 gas CO 2 The concentration is 30% (v / v), and the ventilation rate is 0.1VVm. Before the split culture, after 48 hours of culture, the algae concentration in the photoreactor is as high as 2.0×10 8 cfu / ml, and when the content of nitrogen and phosphorus in the photoreactor meets the national A-class discharge water standard, the fermentation broth is divided, and 1 / 3 to 2 / 3 of the fermentation broth is taken out and concentrated to obtain a concentrated fermentation broth. The concentrated fermentation liquid is returned to the photoreacto...

Embodiment 2

[0047] Using continuous fermentation and using municipal sewage and industrial sewage after filtering out miscellaneous bacteria or miscellaneous algae, the secondary effluent treated by the sewage treatment plant as the medium, inoculate the medium with Chlorella C9-JN2010, wherein, the initial The connection density is 3.5×10 7 cfu / ml, the light cycle is 14h:10h, the light intensity is 5000lux, the culture temperature is 25°C, the pH is 8.5, CO 2 gas CO 2 Concentration is 25.0% (v / v), ventilation rate is 0.2VVm, continuous culture 480h, nitrogen, phosphorus removal effect, CO 2 The conversion rate (% (v / v)) and oil accumulation relationship see Figure 3A-3B shown.

[0048] Depend on Figure 3A The analysis shows that in the early stage of continuous culture, as the culture time prolongs, its response to nitrogen, phosphorus and CO 2 utilization is accelerated. From 0 to 120 hours, the utilization rate of nitrogen increased continuously, and then the removal rate reach...

Embodiment 3

[0051] The municipal sewage and industrial sewage after filtering out miscellaneous bacteria or miscellaneous algae by adopting batch fermentation method are used as the culture medium through the secondary effluent of the sewage treatment plant, and the culture medium is inoculated with Chlorella C9-JN2010, wherein, the initial inoculation Density is 1.0×10 7 cfu / ml, the light cycle is 10h:14h, the light intensity is 7500lux, the culture temperature is 20°C, the pH is 6.5, CO 2 The concentration is 15.0% (v / v), the ventilation rate is 0.01VVm, and the batch culture is 480h, the removal effect of nitrogen and phosphorus, CO 2 The conversion rate (% (v / v)) and oil accumulation relationship see Figures 4A-4B shown.

[0052] Depend on Figure 4A The analysis shows that in the early stage of culture, with the extension of culture time, its response to nitrogen, phosphorus and CO 2 utilization is accelerated. The utilization rate of nitrogen continued to increase within 0-168...

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Abstract

The invention provides a common chlorella, which is chlorella C9-JN2010 with the preservation number of CCTCC NO: M2010373; and the invention further provides a culturing method of the common chlorella. The culturing method comprises the following steps: using secondary outlet water, which is obtained by treating municipal sewage and / or industrial sewage in a sewage treatment plant, as a culture medium; and culturing the chlorella after the chlorella is inoculated, wherein the initial inoculating density is 1.0 multiplied by 107 cfu / ml-6.0 multiplied by 107 cfu / ml, and the culturing conditionis that the light cycle is 10h:14h-16h:8h, the light intensity is 5000-15000 lux, the culturing temperature is 20-30 DEG C, the pH value is 6.5-8.5, the CO2 gas throughput is 0.01-0.2 VVm, and the CO2 concentration is 15.0%-30.0% (v / v). The invention further provides an application of the common chlorella in sewage treatment and oil production. The common chlorella provided by the invention has the advantages of strong CO2 tolerance and high production strength; through the adoption of the culturing method provided by the invention, the sewage treatment and the production of the biological energy are combined with other side products (such as proteins, cosmetic raw materials, and animal feeds), so that the production cost is reduced, the waste resources are comprehensively utilized as well as the energy-saving and emission reducing green production is realized; and the culturing method provided by the invention has the advantages of skillful and special design as well as suitability for being popularized and applied on a large scale.

Description

technical field [0001] The invention relates to the technical field of algae, more specifically to the technical field of chlorella, in particular to a common chlorella and its cultivation method and application. Background technique [0002] Energy shortage, environmental pollution and food shortage are the three major problems in the world today. Global warming attributed to major greenhouse gas CO in the atmosphere 2 The increase in water pollution is one of the major environmental problems along with the ubiquitous water pollution caused by eutrophication substances (eg, nitrogen and phosphorus, etc.) and other pollutants. [0003] Microalgae play a very important role in solving the above problems. Microalgae is a kind of microorganism capable of photosynthetic autotrophy. It is a primary producer in the ecosystem and plays a huge role in the entire material cycle. It has high production efficiency and attractive development prospects. Moreover, microalgae are rich i...

Claims

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Application Information

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IPC IPC(8): C12N1/12C02F3/32C12P7/64C12R1/89
Inventor 杨海麟夏小乐辛瑜张玲王武李昌灵李宇佶
Owner JIANGNAN UNIV
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