Microalga photosynthetic aerobic high-density fermentation culture method utilizing inorganic nitrogen source and application

A high-density fermentation, inorganic nitrogen source technology, applied in microorganism-based methods, fermentation, separation methods, etc., can solve the problems affecting the microalgae inorganic nitrogen assimilation process, affecting the effect of industrial flue gas emission reduction, slow cell growth rate, etc. problems, to achieve the effect of stable and controllable cultivation process, convenient operation and easy method.

Active Publication Date: 2014-05-21
INST OF AQUATIC LIFE ACAD SINICA
View PDF1 Cites 42 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The low biomass and slow cell growth rate of microalgae in the autotrophic culture mode become the "bottleneck" that limits the application of microalgae in flue gas emission reduction and bioenergy production
However, the conventional heterotroph...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microalga photosynthetic aerobic high-density fermentation culture method utilizing inorganic nitrogen source and application
  • Microalga photosynthetic aerobic high-density fermentation culture method utilizing inorganic nitrogen source and application
  • Microalga photosynthetic aerobic high-density fermentation culture method utilizing inorganic nitrogen source and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Prepare medium

[0049] The preparation process of basal medium is as follows:

[0050] Add glucose and NaNO to BG11 medium 3 Prepare basal medium so that the concentration of glucose in the medium is 30g / L, NaNO 3 The content is 4.5g / L;

[0051] The composition of BG11 medium is: NaNO 3 1.5g / L, K 2 HPO 4 ·3H 2 O0.04g / L, MgSO 4 ·7H 2 O0.075g / L, CaCl 2 2H 2 O0.036g / L, citric acid, 0.006g / L, ferric ammonium citrate 0.006g / L, EDTA 2Na, 0.001g / L, Na 2 CO 3 0.02g / L, A 5 Solution 1mL / L; trace element solution A 5 The composition is: H 3 BO 3 2.86g / L, MnCl 2 4H 2 O1.81g / L, ZnSO 4 ·7H 2 O0.222g / L, Na 2 MoO 4 2H 2 O0.390g / L, CuSO 4 ·5H 2 O0.079g / L, CoCl 2 ·6H 2 O0.010g / L.

[0052] The preparation process of feed solution is as follows:

[0053] Add glucose to deionized water so that its concentration is 800g / L to prepare feeding solution 1;

[0054] Add NaNO to deionized water 3 Make its concentration 150g / L to prepare feeding solution 2;

[0055] ...

Embodiment 2

[0060] A microalgae photosynthetic and adjunct culture high-density fermentation culture method using inorganic nitrogen source, taking 3L culture scale as an example, the details are as follows:

[0061] 1. Use a 5L self-controlled light bioreactor (Shanghai Baoxing Biological Equipment Engineering Co., Ltd., BIOTECH-5BGG) for photosynthetic and high-density fermentation of Chlorella, with a culture volume of 3L basal medium. Turn on the photobioreactor and set the culture conditions to an average light intensity of 200 μmol m -2 the s -1 , the temperature is 28°C, compressed air (0.08MPa) is used for ventilation, the ventilation rate is 3.0L / min (that is, the ventilation rate per liter of medium is 1L / min), the stirring speed is 200rpm, and 2M KOH solution and 70% The acetic acid solution automatically adjusts the pH of the culture solution to make the pH 7.0.

[0062] 2. Microalgae inoculation and cultivation. After the culture conditions in the photobioreactor are stabl...

Embodiment 3

[0066] A method for microalgae photosynthetic and trophic high-density fermentation using an inorganic nitrogen source, taking a 30L culture scale as an example, the details are as follows:

[0067] 1. Use a 50L self-controlled light bioreactor (Shanghai Baoxing Biological Equipment Engineering Co., Ltd., BIOTECH-50BSG) for photosynthetic and high-density fermentation of Chlorella, with a culture volume of 30L basal medium. Turn on the photobioreactor and set the culture conditions to an average light intensity of 300 μmol m -2 the s -1 , the temperature is 28°C, compressed air (0.08MPa) is used for ventilation, the ventilation rate is 45.0L / min (that is, the ventilation rate per liter of medium is 1.5L / min), the stirring speed is 200rpm, and 2M KOH solution and 70 % acetic acid solution automatically adjusts the pH value of the culture medium to make the pH 7.0.

[0068] 2. Microalgae inoculation and cultivation. After the culture conditions in the photobioreactor were sta...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a microalga photosynthetic aerobic high-density fermentation culture method utilizing an inorganic nitrogen source and an application. The invention relates to a microalga high-density fermentation culture method utilizing the inorganic nitrogen source, wherein photosynthetic aerobic culture is performed in a culture medium in which inorganic nitrogen salt is taken as the inorganic nitrogen source and glucose is taken as an organic carbon source; glucose, inorganic nitrogen salt and other nutrient salts are segmentally replenished according to consumption of the nitrogen source and carbon source of the culture medium and the biomass growth condition; the concentration of the nutrient salts, pH value, ventilatory capacity, light intensity and alga liquid stirring speed are reasonably regulated, so that microalgae can be rapidly proliferated in a short time, the culture time is shortened, high-density growth of the microalgae is guaranteed, rapid growth of the biomass of microalga cells is realized, and high-efficiency absorption and assimilation of inorganic nitrogen are realized. The method disclosed by the invention is suitable for high-density photosynthetic aerobic fermentation production of the microalgae by utilizing the inorganic nitrogen salt as a nitrogen source, and the method lays the foundation of an economic feasibility research on environmental and ecological applications and bioenergy production of the microalgae.

Description

technical field [0001] The invention belongs to the fields of microalgae biotechnology, environmental ecological technology and bioenergy, and relates to a microalgae photosynthesis and high-density fermentation culture method using inorganic nitrogen sources and its application, which can be used for high-density fermentation culture of microalgae using inorganic nitrogen sources , the reduction of NOx emissions from industrial flue gas by microalgae and the collaborative production of microalgae bioenergy raw materials. Background technique [0002] Microalgae are prokaryotic or eukaryotic single-celled photosynthetic microorganisms. They are very efficient solar energy converters. They are distributed in fresh or salt water and absorb light energy, water and CO 2 Accumulated biomass can convert light energy into chemical energy and store it in the cell in the form of organic matter such as oil or starch. As the oldest lower photosynthetic organisms, some microalgae can d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/12C12P7/64B01D53/84B01D53/56C12R1/89
CPCY02A50/20
Inventor 王强陈辉何晨柳
Owner INST OF AQUATIC LIFE ACAD SINICA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap