Microalga photosynthetic aerobic high-density fermentation culture method utilizing inorganic nitrogen source and application

Abstract

The invention discloses a microalga photosynthetic aerobic high-density fermentation culture method utilizing an inorganic nitrogen source and an application. The invention relates to a microalga high-density fermentation culture method utilizing the inorganic nitrogen source, wherein photosynthetic aerobic culture is performed in a culture medium in which inorganic nitrogen salt is taken as the inorganic nitrogen source and glucose is taken as an organic carbon source; glucose, inorganic nitrogen salt and other nutrient salts are segmentally replenished according to consumption of the nitrogen source and carbon source of the culture medium and the biomass growth condition; the concentration of the nutrient salts, pH value, ventilatory capacity, light intensity and alga liquid stirring speed are reasonably regulated, so that microalgae can be rapidly proliferated in a short time, the culture time is shortened, high-density growth of the microalgae is guaranteed, rapid growth of the biomass of microalga cells is realized, and high-efficiency absorption and assimilation of inorganic nitrogen are realized. The method disclosed by the invention is suitable for high-density photosynthetic aerobic fermentation production of the microalgae by utilizing the inorganic nitrogen salt as a nitrogen source, and the method lays the foundation of an economic feasibility research on environmental and ecological applications and bioenergy production of the microalgae.

Description

technical field [0001] The invention belongs to the fields of microalgae biotechnology, environmental ecological technology and bioenergy, and relates to a microalgae photosynthesis and high-density fermentation culture method using inorganic nitrogen sources and its application, which can be used for high-density fermentation culture of microalgae using inorganic nitrogen sources , the reduction of NOx emissions from industrial flue gas by microalgae and the collaborative production of microalgae bioenergy raw materials. Background technique [0002] Microalgae are prokaryotic or eukaryotic single-celled photosynthetic microorganisms. They are very efficient solar energy converters. They are distributed in fresh or salt water and absorb light energy, water and CO 2 Accumulated biomass can convert light energy into chemical energy and store it in the cell in the form of organic matter such as oil or starch. As the oldest lower photosynthetic organisms, some microalgae can d...

AI Technical Summary

Problems solved by technology

The low biomass and slow cell growth rate of microalgae in the autotrophic culture mode become the "bottleneck" that limits the application of microalgae in flue gas emission reduction and bioenergy production

However, the conventional heterotrophic culture mode of microalgae using organic carbon sources under the condition of no light significantly affects the assimilation process of microalgae inorganic nitrogen, and affects the emission reduction effect of industrial flue gas, especially NOx.

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