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Ligno-cellulose material pre-treatment method capable of obtaining conversable substrates

A lignocellulose and pretreatment technology, applied in the field of microorganisms, can solve problems such as reducing biomass resistance, achieve the effects of reducing consumption, improving yield and quality, and reducing production costs

Inactive Publication Date: 2009-03-25
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the use of basidiomycete pretreatment to reduce the resistance of biomass to cellulase reactions and promote the production of transformable substrates such as biorefinery and biomass energy.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 Basidiomycetes or flora solid bacterial classification preparation

[0024] Basidiomycete strains with significant biomass destruction ability such as Echinodontium sp., Trichaptum sp., Irpex sp. and significant hemicellulose degrading basidiomycete strains Schizophyllum sp., Polyporellus sp, Poria sp. slant strains preserved in the refrigerator were inserted into Carry out activation on the potato slant, and cultivate for 5-7 days. After the mycelium is full of the slant, take the mycelium slant block and insert it into the straw (such as corn stalk) medium for cultivation, and culture it at 26°C for 5-7 days, and then carry out shallow Plate expansion culture 6 ~ 8d, that is, the solid strains for open use.

Embodiment 2

[0025] Embodiment 2 Basidiomycete or flora continuous open treatment (1)

[0026] Prepare strains according to the method of Example 1: Echinodontium sp., Trichaptum sp, Irpex sp, Schizophyllum sp, Polyporellus sp. Under open conditions, the prepared strains were respectively inoculated onto unsterilized corn stalks (crushed to 20-40 mesh), the corn stalk:water W / V ratio was 1:2.5, the pH was natural, and the inoculation amount was 10% (W / W)) The culture medium is mixed evenly, left to ferment at 25-28°C for 10-14 days, and 80-90% (W / W) of the fermented substrate is taken out and dried to obtain a transformable substrate; 10% to 20% (W / W) process fermentation substrate, and then add 80% to 90% (W / W) fresh slag to mix and ferment evenly, and continue to cycle pretreatment for half a year.

Embodiment 3

[0027] Embodiment 3 Basidiomycetes or flora continuous open treatment (two)

[0028] Prepare strains according to the method of Example 1: Echinodontium sp. Under open conditions, mix the prepared strains with unsterilized hyphae hyphae slag (the slag:water W / V ratio is 1:2.5, the pH is natural, and the inoculum size is 15%). Leave it to ferment for 10-14 days at ℃, take out 80-90% (W / W) of the fermented substrate and dry it for the acquisition of convertible substrates; take 10%-20% (W / W) of the fermented Add 80% to 90% (W / W) of fresh slag to the substrate, mix and ferment evenly, and continue to cycle pretreatment for half a year.

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PUM

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Abstract

The invention provides a pretreatment method for obtaining ligno-cellulose materials of a transformable substrate required in the production of bio-refinery, bio-energy, biological medicine, food processing, light chemical products, biological feedstuff and fertilizers, which adopts baisdiomycete strains or flora that can selectively destroy the structure of ligno-cellulose to carry out continuous pretreatment of solid fermentation to various ligno-cellulose materials in an open condition. The transformable substrate can be obtained directly and simply by extraction after the pretreatment or obtained by combining the treatment of the physical and chemical biomass white-rot fungus degradation system with the complex enzymatic method. The method of the invention puts biological degradation, modification and biomass transformation in leading position, partially combines with the physical and chemical enzymatic treatment process technology and has the advantages of strong operability, wide application scope, and the like.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a biological pretreatment method for obtaining a convertible substrate that can be used for various product conversions from lignocellulosic raw materials. Background technique [0002] With the depletion of fossil resources, the search for new alternative energy sources and the acquisition of biorefinery and light chemical raw materials from non-petroleum routes have become research hotspots. Biorefinery and biochemical industries dominated by biomass energy have attracted much attention due to their cleanliness and environmental protection. . At present, starchy biomass such as aged grains, corn, wheat, and potatoes are mainly used as the main raw materials for the production of biorefinery, biochemical industry, biomedicine, food fermentation, and biological feed. The long-term use of grain as raw material involves competition with others for grain and land, high costs...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12P19/14C12R1/645
CPCY02E50/343Y02E50/30
Inventor 张晓昱余洪波徐春燕柯静邬键国
Owner HUAZHONG UNIV OF SCI & TECH
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