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Microorganism fermentation grease and method for preparing organism diesel oil from the same

A microbial fermentation and microbial oil technology, applied in the preparation of liquid hydrocarbon mixtures, biological raw materials, biofuels, etc., to achieve the effects of easy process control, low cost, and abundant raw materials

Inactive Publication Date: 2007-03-07
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention combines the technology of microbial fermentation to produce microbial oil and the technology of transesterification to prepare biodiesel, and proposes a microbial fermentation of oil and its method for preparing biodiesel, which has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 100ml of microbial oil fermentation medium is placed in a 500ml Erlenmeyer flask, connected with 5% yeast strains, and fermented for 60 hours at a temperature of 30°C at a shaker speed of 180r / min, and then centrifuged to remove the thallus to obtain Microbial fermented oil with a content of 6.5g / L.

Embodiment 2

[0024] Inoculate 5% of the inoculum into a 1L fully automatic agitated fermenter with a liquid volume of 70%, at a temperature of 30°C, with a ventilation rate of 1vvm, control the pH of the culture environment = 6.0, and stir at a speed of 300r / min, after 60 hours of fermentation , remove the thallus after centrifugation, and obtain the microbial fermented oil with a content of 8g / L.

Embodiment 3

[0026] The microbial oil was extracted by methanol-chloroform method. Take 100mg of microbial oil, add 2ml of 0.4mol / L KOH-methanol solution and 2ml of n-hexane, shake vigorously for 2min, place it in a water bath at 40°C for 15min, add 5ml of water, let it stand for layers, take the n-hexane layer, and use gas chromatography and The components were analyzed by mass spectrometry. The specific analysis results are shown in Table 1. It can be seen from the analysis results that the main components of oil fermented by yeast microorganisms are similar to those of vegetable oil, and can be used for the preparation of biodiesel.

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PUM

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Abstract

The invention discloses a biological diesel preparing method of fat fermented by microbe in the biological energy and biological catalytic technological domain, which comprises the following steps: grafting yeast microbe in the fat-producing bacterial culture medium; centrifuging; separating; extracting to obtain the fat; esterifying under catalyst to obtain biological diesel and glycerine as by-product.

Description

technical field [0001] The invention belongs to the technical field of bioenergy and biocatalysis, and in particular relates to a microbial fermentation oil and a method for preparing biodiesel. Background technique [0002] At present, the growth of population has made the contradiction between the increasing oil demand and the serious shortage of natural resources increasingly acute, especially with the increasingly serious global energy shortage and environmental deterioration, people have to start from the perspective of environmental protection and resource development , Actively develop renewable new energy to replace fossil fuels, such as biodiesel (fatty acid methyl ester) is a renewable and clean energy with great development potential and environmental friendliness. Biodiesel has excellent properties expected from liquid energy products, such as energy density, low sulfur content, sufficient combustion, and good lubrication performance. Since 1988, many European c...

Claims

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Application Information

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IPC IPC(8): C10G3/00
CPCY02E50/13Y02E50/10Y02P30/20
Inventor 张建安刘宏娟墨玉欣程可可周玉杰杜伟刘德华
Owner TSINGHUA UNIV
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