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Combination bacteriostat for selectively culturing HP (helicobacter pylori) and preparation method of combination bacteriostat

A Helicobacter pylori and selective technology, applied in the field of preparation of the mixed bacteriostatic agent, can solve the problems of cumbersome cultivation process, high technical requirements for cultivation, many growth of miscellaneous bacteria and the like

Active Publication Date: 2012-07-11
厦门赋源康实业有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0013] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a mixed bacteriostatic agent for selective cultivation of Helicobacter pylori, to solve the problem of harsh growth conditions for Helicobacter pylori cultivation, cumbersome cultivation process, high cultivation technology requirements, miscellaneous The problem of bacterial growth

Method used

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  • Combination bacteriostat for selectively culturing HP (helicobacter pylori) and preparation method of combination bacteriostat
  • Combination bacteriostat for selectively culturing HP (helicobacter pylori) and preparation method of combination bacteriostat
  • Combination bacteriostat for selectively culturing HP (helicobacter pylori) and preparation method of combination bacteriostat

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Embodiment 1

[0043] Embodiment 1: the preparation method of the selective culture Helicobacter pylori mixed bacteriostatic agent of formula 1, comprising:

[0044] 1) Preparation of medium (500ml):

[0045] Take 465 ml of brain-heart infusion medium, 40 ml of fetal bovine serum, 0.5 g and 0.02 g of β-CD, add 500 ml of distilled water, and adjust the pH to 7.2-7.4 with 40% NaOH;

[0046] 1.1) Prepare the culture medium according to the above formula, put it into three 500 ml Erlenmeyer flasks (167ml / bottle), and number them as blank, I, II respectively, seal the bottle mouth tightly with a parafilm, and sterilize under high pressure at 121°C for 15~ After 30 min, after the pressure in the autoclave dropped to 0, take out the culture bottle and let it cool down naturally;

[0047] 1.2) Thaw sheep blood and inactivate complement at 56°C for 30 min;

[0048] 1.3) Cool the above-mentioned medium in step 1.1) to about 40°C, except for the blank, add 100ul of pre-prepared selective bacteriostatic...

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Abstract

The invention provides a combination bacteriostat for selectively culturing HP (helicobacter pylori), the formula of the combination bacteriostat is that: 3-50mg / l of vancomycin, 5-10.0mg / l of nalidixic acid, 3-5.0mg / l of TMP, 1-2.0mg / l of amphotericin and 8-10mg / l of Nisin. The preparation method of the combination bacteriostat for selectively culturing the HP mainly comprises the following procedures of culture medium preparation and bacteria inoculation, culture and preservation. According to the combination bacteriostat containing the amphotericin B, the nalidixic acid, the vancomycin, the TMP and the Nisin, through the addition of the combination bacteriostat in a culture medium, not only can the pollution of mixed bacteria such as bacillus subtilis, gram positive cocci and mucedine be reduce, but also thymidine needed by the growth of the HP can be provided for the HP to inhibit the growth of the mixed bacteria.

Description

Technical field [0001] The invention involves a hybrid antibacterial agent and preparation method for selectively cultivating Helicobacter pylori.Specifically, the use of mixed bacteriostatic bisexilin B, pyrine, pyromycin, vanomycin, and TMP are added to the growth of mixture.The present invention also involves the preparation method of the hybolic antibacterial agent. [0002] Background technique [0003] Helicobacter Pylori (HP) is a curved Gram -negative bacteria that causes the main pathogenic bacteria of digestive tract inflammation, ulcer, gastric mucosa -related lymphoma (MALT) lymphoma, and is directly related to the occurrence of gastric cancer.It was listed as a first -class carcinogenic factor by the International Health Organization.About 50 % of the world's gastric mucosa infection has HP infection. How to eradicate the digestive system disease caused by HP has become the focus of attention of scholars in the digestive industry.HP's growth conditions are very harsh...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 胥秀英郑一敏周慧王琳琳乐亮江娟林海珠王宁刘红张云玲胡少南
Owner 厦门赋源康实业有限公司
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