Group B Streptococcus Enrichment Broth and Its Application

A technology of enrichment broth and streptococcus, applied in bacteria, microorganisms, microorganism-based methods, etc., can solve the problems of high missed detection rate of GBS, inability to effectively identify pregnant women, etc., and improve the detection rate and detection rate. High, the effect of inhibiting the growth of miscellaneous bacteria

Active Publication Date: 2022-02-11
GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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  • Description
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Problems solved by technology

In China, the detection rate of GBS in Guangzhou Women and Children's Medical Center is slightly higher (about 10%), 7-8% in Beijing and Zhejiang, and as low as 1.03% in Shenyang, which shows that the detection rate of GBS in pregnant women at 35 to 37 weeks in my country is relatively high. GBS has a high rate of missed detection and cannot effectively identify pregnant women who should receive prophylactic antimicrobial therapy

Method used

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  • Group B Streptococcus Enrichment Broth and Its Application
  • Group B Streptococcus Enrichment Broth and Its Application
  • Group B Streptococcus Enrichment Broth and Its Application

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Embodiment

[0047] Comparing the bacterial enrichment effect of T-H broth, T-V broth, LIM broth, enrichment broth I, enrichment broth II and enrichment broth III, enrichment broth I is the B group streptococcus enrichment provided by the present invention. mushroom broth. The formula of each enrichment broth is as shown in Table 1:

[0048] Table 1. The formula of each group of enrichment broth

[0049] broth name T-H Broth Powder Gentamicin nalidixic acid Polymyxin B T-H Broth √ - - - T-V Broth √ 8μg / mL 15μg / mL - lim broth √ - 15μg / mL 10μg / mL Enrichment Broth Ⅰ √ - 30μg / mL 10μg / mL Enrichment Broth II √ - - 8μg / mL Enrichment Broth III √ - - 10μg / mL

[0050] The preparation method of each enrichment broth is as follows:

[0051] T-H broth: Todd-Hewitt broth dry powder (Shanghai Guandao Bioengineering Co., Ltd.) was heated and dissolved in deionized water, and sterilized in a high-pressure steam sterilizer...

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Abstract

The invention provides a group B streptococcus enrichment broth and application thereof, and relates to the technical field of microorganism cultivation. The group B streptococcal enrichment broth includes Todd-Hewitt broth, nalidixic acid and polymyxin B; wherein the concentration of nalidixic acid is 28-32 μg / mL; the concentration of polymyxin B is 8-12 μg / mL. The Group B Streptococcus enrichment broth can increase the quantity of Group B Streptococcus in the inoculum, inhibit the growth of miscellaneous bacteria, and improve the detection rate of Group B Streptococcus.

Description

technical field [0001] The invention relates to the technical field of microorganism culture, in particular to a group B streptococcus enrichment broth and application thereof. Background technique [0002] Group B Streptococcus (GBS), also known as Streptococcus agalactiae, is a Gram-positive bacterium that normally colonizes the lower reproductive tract and digestive tract of women. GBS is the main cause of serious infections such as neonatal pneumonia, sepsis and encephalitis worldwide. Mother-to-child transmission is the main route of neonatal infection, especially when newborns swallow and inhale GBS bacteria during delivery through the birth canal, which can seriously cause neonatal death or neurological sequelae. Since the 1990s, Western developed countries in Europe and the United States have effectively reduced early-onset GBS infections in newborns aged 0-6 days by implementing and improving prenatal GBS screening and intrapartum antibiotic intervention (IAP). Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/04C12R1/46
CPCC12N1/20C12Q1/04
Inventor 刘海英邓秋连叶家辉钟华敏高坎坎黄莲芬谢永强
Owner GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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